Arhgap45 (NM_027521) Mouse Tagged ORF Clone

CAT#: MG211418

Hmha1 (tGFP-tagged) - Mouse histocompatibility (minor) HA-1 (Hmha1)

Product Images

Circular map for MG211418

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgap45
• Synonyms: 6330406L22Rik; AW539505; Ha-1; Hmha1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211418 representing NM_027521
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTCTGTGGGACTCTGCATCCAGCGCTGGACCATGACCCCCTGTGCTGCCAGACTCGCGCCCGAG
  ACCTTCTCGAGGCCCGCCGGCCACTGGCCCATGAGTGCCTGGGTGAAGCTCTGCGAGTCATGCGCCAGGT
  CATCTCCAGATACCCACTCCTCAACACCGTGGAGACACTCACAGCCGCTGGTACACTCATTGCCAAGGTC
  AAAGCCTTTCATTATGAATGCAACAATGAATCAGATAAGAGGGAATTTGAGAAGGCTCTGGAAACCATTG
  CGGTGTCCTTCAGCTGCACTGTGTCCGAGTTCCTTTTGGGTGAAGTGGACAGCAGTACTCTCCTGGCGGT
  GCCTCCGGGGGACCCCAGCCAGTCCATGGAGAACCTTTATGGGGCTGGCACGGAGGGGCCCCCACATAAC
  GTGGAGGAATGTGAAGAAGGTTGCCTGCCCCCCGAAGAGGTGGACATGCTTCTGCAGCGATGCGAAGGGG
  GTGTGGACGCTGCACTGCAGTATGCAAAAGACATGGCCAGGTACATGAAGGACCTCATCAGCTACCTGGA
  GAAGAGGACCACCCTGGAGATGGAATTCGCCAAAGGTCTCCAGAAGGTTGTCCACAACTGCAGACAGAGC
  GTCACACACGAGCCCCACATGCCTCTCTTGTCCATCTACTCACTGGCCCTGGAACAAGATTTGGAGTTTG
  GCCACGGCATGGTGCAGGCAGCGGGCACACTGCAGACCCAGACCTTCATGCAGCCCCTGACGCTGCGGCG
  GTTGGAGCATGAGAGGCGCAGGAAGGAGATCAAAGAATCTTGGCATCGAGCACAGAGGAAGCTGCAAGAG
  GCAGAGGCCAACCTGCGCAAGGCCAAACAGGGCTACAAACAACGCTGTGAAGACCACGACAAGGCCCGGC
  TCCAGGTGGCCAAGGCTGAGGAGGAACAACAGGGCACAGGCCCAGGCGCAGGGACTGCGGCCTCTAAGGC
  CCTGGACAAGAGGCGCAGACTGGAGGAAGAGGCCAAAAACAAGGCCGAGGAGGCCATGGCCACTTACCGC
  ACATGCGTGGCAGACGCAAAGACACAGAAGCAGGAGCTGGAGGACACAAAGGTGACTGCGCTGCGGCAGA
  TTCAGGAGGTCATCAGGCAGAGTGATCAGACCATTAAGTCGGCCACCATCTCCTACTACCAGCTGATGCA
  CATGCAGACAGCACCGCTGCCCGTGAACTTCCAGATGCTGTGCGAGAGCAGCAAACTATATGACCCGGGC
  CAACAGTACGCATCTCACGTGCGTCAGCTGCAGAGAGGCGAGGAGCCGGACGTGCGCTACGACTTTGAGC
  CTTATGTCTCTAACAACTCCTGGTCCCCAATCATGCGTACACGGAAGGGCAGCTTCAACCCTGGTGATGC
  TTCAGGACCCGAAGCTGCTGGTAGTCCCCCCGAGGAAGGTGGCACCTCTGAGGCAGCACCCAACAAGGAC
  CACAGGGGGGGACGAGGTCACCAGGTTCATAAGTCCTGGCCCATCTCCATCTCAGACACCGAAGTTGGTC
  TGGACACCAGCTCAGGGGACTTGAAGAAGTTCGATCGAACATCTTCCAGTGGGACGATGTCATCCAGTGA
  GGAGCTGGGGGATCAGGAAGCTGGCTTGGTAGCTTCCGCCTTTGATTCAGCTGACCTCAATGGCATGGAC
  CCCGAGTTACCTGTGGCCATGCCCAGTGGACCCTTCCGCCATGTGGGATTGTCCAAGGCAGCCCGCACAC
  ACCGACTTCGCAAACTGCGCACGCCAGCCAAGTGCAGAGAGTGTAACAGCTATGTGTACTTCCAAGGAGC
  TGAATGTGAAGAGTGCTGTCTGGCTTGTCACAAAAAGTGTTTGGAGACCCTGGCCATCCAGTGTGGCCAC
  AAGAAGCTTCAGGGCCGCCTGCAGCTCTTTGGACAAGACTTCAGCCAGGCAGCCCTCAGCACCCCTGATG
  GGGTGCCCTTCATTGTCAAAAAATGTGTCTGTGAGATTGAGCGGCGGGCACTGCACACCAAGGGGATCTA
  CCGGGTCAATGGCGTGAAAACGCGTGTGGAGAAGCTGTGCCAGGCTTTTGAGAATGGCAAAGAGTTGGTG
  GAGTTGTCACAGGCCTCACCCCACGACATCAGCAACGTCCTAAAGCTATACCTGCGGCAGCTACCGGAGC
  CCCTTATCTCTTTTCGCTTCTACCATGAGCTGGTGGGACTAGCTAAGGACAGCCTAAAGGCCGAAGCCGA
  AGCTAAGGCAGCGAGCCGGGGTCGGCAGGGTGGGTCCGAGAGTGAGGCTGCAACCTTGGCTATGGTGGGC
  CGCCTGCGTGAGCTCATGCAGGATCTGCCAGCCGAAAACCGGGCCACACTCTTATACCTGCTGAAGCACT
  TGCGAAGGATCGTGGAAATGGAGCAGGATAACAAGATGACCCCTGGGAACCTGGGCATCGTTTTCGGGCC
  CACACTGTTGCGGCCTCGACCCACTGACGCCACCGTGTCCCTCTCTTCTCTGGTGGATTATCCCCACCAG
  GCCCGAGTTATTGAGACTCTGATTGTCCACTACGGCCTGGTCTTTGAGGAGGAGCCAGAGGAGGCGCCTG
  GCAGCCAGGAGGGGGCATCCACCCAGTGTGGCCAGCTGGAGTCTGCTGAGGGCATTGTCTTCCCCCTGCA
  GGAGGAGGCAGAGGACGGAAGCCGAGAATCTCACGCGGCATCTAATGACTCGGACTCTGAGCTGGAAGAC
  GCTTCTGACCCCCTTTCATCCTCGGACGCCAGTGCTCTACACCGCCTCAGTTTCCTGGAGCAGACCGAGG
  CAGGCCTGGAGGAAGGTCCCCAGAGCCATAGTGGCAGTGAGGAACAGCTGGAGGGTGAGGATGGAGCCCC
  GGGTCCGTGGCTGTGTCACTTCAACACCAACCAGTCCAACAACACATCACGGGCCCCTCTGCCCACCATG
  CGGCTCCGAGGAGGGCAGATCACAGGTGGCACCAGTCAGGAGCGGCAGCCACAGTTCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211418 representing NM_027521
  Red=Cloning site Green=Tags(s)
  
  MCVCGTLHPALDHDPLCCQTRARDLLEARRPLAHECLGEALRVMRQVISRYPLLNTVETLTAAGTLIAKV
  KAFHYECNNESDKREFEKALETIAVSFSCTVSEFLLGEVDSSTLLAVPPGDPSQSMENLYGAGTEGPPHN
  VEECEEGCLPPEEVDMLLQRCEGGVDAALQYAKDMARYMKDLISYLEKRTTLEMEFAKGLQKVVHNCRQS
  VTHEPHMPLLSIYSLALEQDLEFGHGMVQAAGTLQTQTFMQPLTLRRLEHERRRKEIKESWHRAQRKLQE
  AEANLRKAKQGYKQRCEDHDKARLQVAKAEEEQQGTGPGAGTAASKALDKRRRLEEEAKNKAEEAMATYR
  TCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQLMHMQTAPLPVNFQMLCESSKLYDPG
  QQYASHVRQLQRGEEPDVRYDFEPYVSNNSWSPIMRTRKGSFNPGDASGPEAAGSPPEEGGTSEAAPNKD
  HRGGRGHQVHKSWPISISDTEVGLDTSSGDLKKFDRTSSSGTMSSSEELGDQEAGLVASAFDSADLNGMD
  PELPVAMPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGH
  KKLQGRLQLFGQDFSQAALSTPDGVPFIVKKCVCEIERRALHTKGIYRVNGVKTRVEKLCQAFENGKELV
  ELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGLAKDSLKAEAEAKAASRGRQGGSESEAATLAMVG
  RLRELMQDLPAENRATLLYLLKHLRRIVEMEQDNKMTPGNLGIVFGPTLLRPRPTDATVSLSSLVDYPHQ
  ARVIETLIVHYGLVFEEEPEEAPGSQEGASTQCGQLESAEGIVFPLQEEAEDGSRESHAASNDSDSELED
  ASDPLSSSDASALHRLSFLEQTEAGLEEGPQSHSGSEEQLEGEDGAPGPWLCHFNTNQSNNTSRAPLPTM
  RLRGGQITGGTSQERQPQFV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027521
• ORF Size: 3000 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027521.3, NP_081797.1
• RefSeq Size: 3480 bp
• RefSeq ORF: 3003 bp
• Locus ID: 70719
• UniProt ID: Q3TBD2
• Cytogenetics: 10 C1
• Gene Summary: Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function]