Slitrk3 (NM_198864) Mouse Tagged ORF Clone

CAT#: MG211375

Slitrk3 (tGFP-tagged) - Mouse SLIT and NTRK-like family, member 3 (Slitrk3)

Product Images

Circular map for MG211375

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slitrk3
• Synonyms: ST3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211375 representing NM_198864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGCCAAGCATAGCTGAAATGCTTCACAGAGGAAGAATGCTGTGGATAATTCTTCTAAGCACAA
  TTGCTCTAGGATGGACTACCCCAATTCCCCTGATAGAGGACTCAGAAGAAATAGATGAGCCCTGTTTTGA
  TCCATGCTACTGCGAAGTTAAAGAAAGCCTCTTTCACATACATTGTGACAGCAAGGGATTTACAAACATT
  AGTCAGATTACTGAGTTCTGGTCAAGACCTTTTAAACTGTATCTTCAGAGGAATTCGATGAGAAGACTAT
  ACACAAACAGTTTTCTTCACTTGAATAATGCGGTGTCCATTAACCTTGGGAACAATGCATTGCAGGACAT
  TCAAACTGGAGCATTTAATGGCCTTAAGATTTTAAAGAGACTGTATCTCCATGAGAACAAACTAGATGTC
  TTCAGAAATGACACCTTCCTTGGCTTAGAAAGTCTGGAATATCTTCAGGCAGATTACAATGTCATTAAAC
  GTATAGAGAGTGGGGCATTTCGGAACCTAAGTAAATTGAGGGTTCTAATCTTAAATGATAATCTCATCCC
  CGTGCTTCCAACCAATTTATTTAAAGCTGTTTCCTTAACGCATCTGGACCTTCGTGGGAACAGGTTAAAG
  GTTCTCTTCTACCGTGGGATGCTGGATCACATTGGCAGAAGCCTGATGGAGCTCCAGCTAGAAGAGAATC
  CTTGGAACTGTACCTGTGAGATAGTACAGCTGAAGAGTTGGCTGGAGCGAATACCTTACACAGCCCTGGT
  GGGAGACATTACCTGTGAGACACCTTTCCATTTCCATGGGAAGGATCTCCGTGAAATCAAGAAGACTGAA
  CTCTGTCCACTGTTGTCTGACTCTGAGGTGGAGGCCAGTCTGGGGATTCCCCACTTATCTTCAAGCAAGG
  AGAATGCCTGGCCAACTAAACCTTCCTCTATGTTGTCTTCTGTCCATTTTACTGCTTCATCTGTTGAATA
  TAAGTCCTCTAATAAGCAACCAAAACCCACCAAACAGCCCAGAACACCGAGACCACCTTCGACATCCCAG
  GCTTTATATCCTGGTCCAAATCAACCTCCTATTGCCCCCTATCAGACTAGACCACCCATTCCCATTATAT
  GTCCTACTGGGTGTACATGCAATTTGCATATCAATGACCTTGGCTTGACTGTCAACTGCAAAGAAAGAGG
  ATTTAACAACATCTCTGAACTCCTTCCAAGGCCCTTGAATGCCAAGAAGCTGTACCTGAGCAGCAATCTT
  ATTCAGAAGATATACCGCTCTGATTTTTGGAATTTTTCTTCTTTGGATCTCTTGCACCTAGGGAACAATC
  GCATTTCCTACGTCCAGGATGGGGCTTTCATCAACCTGCCCAACCTGAAGAGTCTCTTTCTCAATGGCAA
  TGATATAGAAAAGCTGACTCCAGGTATGTTCCGAGGTCTGCAGAGCTTGCATTACTTGTACTTCGAATTC
  AACGTCATCCGGGAAATCCAACCTGCAGCCTTCAGCCTCATGCCCAACTTGAAGCTGCTATTTCTCAACA
  ATAACTTGCTGAGGACTCTGCCAACTGATGCCTTTGCAGGTACATCCCTGGCTCGGCTCAACTTGAGGAA
  GAACTACTTTCTCTACCTGCCAGTGGCTGGTGTCCTTGAACACCTGAATGCCATTGTGCAGATAGATCTC
  AACGAGAATCCTTGGGACTGCACTTGCGACTTAGTCCCCTTTAAGCAGTGGATCGAAACCATCAGCTCAG
  TCAGCGTGGTAGGTGATGTACTCTGTAGGACCCCCGAGAACCTCACCCACCGTGATGTGCGCACTATTGA
  GCTGGAAGTTTTATGCCCAGAGATGCTGCATATTGCCCAACCTGGACCATCTCCACCCCAGCCTGGAGAT
  TACCACCCTAACGGGGGACCAACAAGTGCATCACCTTATGAGTTCTCTCCCCCAGGGGGCCCTGTGCCAC
  TTTCTGTGTTGATTCTCAGCCTCTTGGTTCTGTTCTTCTCAGCTGTCTTTGTTGCTGCAGGCCTCTTTGC
  CTATGTGCTCCGTCGGCGTAGGAAGAAGTTGCCCTTTAGAAGCAAGAGACAAGAAGGTGTGGACCTTACA
  GGAATCCAGATGCAATGTCACCGGCTGTTTGAGGACAGTGGAGGCAACAGTGGTGGGAGTGGAGGTGGAG
  GGCGACCAACTCTGTCCTCGCCAGAGAAAGCCCCTCCCGTGGGTCACGTGTACGAGTATATACCCCACCC
  AGTAACTCAGATGTGTAACAACCCCATCTATAAGCCTCGAGAGGAGGAAGAAGTGGCTGCCTCAGCAGCC
  CAGGACACAGGAGCTACAGACCGTGGAGGTCCTGGTACACAACCAACAGGAATGGCTGAGGTGCTCTTGG
  GAAGTGAGCAGTTTGCAGAAACACCCAAGGAGAACCACAGTAACTACAGGACCTTGCTGGAAAAAGAGAA
  GGAGTGGGCTCTGGCTGTTTCCAACTCTCAACTTAACACCATAGTGACGGTGAACCATCACCACCCTCAC
  CCTCACCACTCGGCAGTTGGTGGGGTTTCAGGGGTAGGTGGAGGGACTGGGGGAGACTTGGCTGGGTTCC
  GCCACCATGAGAAGAATGGTGGGGTGGTGCTGTTTCCCCCTGGGGGAGGTTGTGGTGGTGGCAGTCTGCT
  ACTAGACCGTGAGAGGCCACAGCCTGCTCCGTGCACAGTGGGATTTGTGGACTGTCTCTATGGCACAGTG
  CCCAAATTAAAGGAACTCCATGTCCACCCCCCTGGCATGCAATATCCAGACTTACAGCAGGATGCCAGGC
  TCAAAGAAACCCTTCTCTTCTCAGCTGGAAAGGGCTTCACAGACCACCAAACCCCCAAAAGTGATTACCT
  CGACTTAAGGGCCAAACTTCAAACTAAGCCGGATTACCTCGAAGTTCTGGAGAAAACAGCATACAGGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211375 representing NM_198864
  Red=Cloning site Green=Tags(s)
  
  MMKPSIAEMLHRGRMLWIILLSTIALGWTTPIPLIEDSEEIDEPCFDPCYCEVKESLFHIHCDSKGFTNI
  SQITEFWSRPFKLYLQRNSMRRLYTNSFLHLNNAVSINLGNNALQDIQTGAFNGLKILKRLYLHENKLDV
  FRNDTFLGLESLEYLQADYNVIKRIESGAFRNLSKLRVLILNDNLIPVLPTNLFKAVSLTHLDLRGNRLK
  VLFYRGMLDHIGRSLMELQLEENPWNCTCEIVQLKSWLERIPYTALVGDITCETPFHFHGKDLREIKKTE
  LCPLLSDSEVEASLGIPHLSSSKENAWPTKPSSMLSSVHFTASSVEYKSSNKQPKPTKQPRTPRPPSTSQ
  ALYPGPNQPPIAPYQTRPPIPIICPTGCTCNLHINDLGLTVNCKERGFNNISELLPRPLNAKKLYLSSNL
  IQKIYRSDFWNFSSLDLLHLGNNRISYVQDGAFINLPNLKSLFLNGNDIEKLTPGMFRGLQSLHYLYFEF
  NVIREIQPAAFSLMPNLKLLFLNNNLLRTLPTDAFAGTSLARLNLRKNYFLYLPVAGVLEHLNAIVQIDL
  NENPWDCTCDLVPFKQWIETISSVSVVGDVLCRTPENLTHRDVRTIELEVLCPEMLHIAQPGPSPPQPGD
  YHPNGGPTSASPYEFSPPGGPVPLSVLILSLLVLFFSAVFVAAGLFAYVLRRRRKKLPFRSKRQEGVDLT
  GIQMQCHRLFEDSGGNSGGSGGGGRPTLSSPEKAPPVGHVYEYIPHPVTQMCNNPIYKPREEEEVAASAA
  QDTGATDRGGPGTQPTGMAEVLLGSEQFAETPKENHSNYRTLLEKEKEWALAVSNSQLNTIVTVNHHHPH
  PHHSAVGGVSGVGGGTGGDLAGFRHHEKNGGVVLFPPGGGCGGGSLLLDRERPQPAPCTVGFVDCLYGTV
  PKLKELHVHPPGMQYPDLQQDARLKETLLFSAGKGFTDHQTPKSDYLDLRAKLQTKPDYLEVLEKTAYRF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198864
• ORF Size: 2940 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198864.3
• RefSeq Size: 3751 bp
• RefSeq ORF: 2943 bp
• Locus ID: 386750
• UniProt ID: Q810B9
• Cytogenetics: 3 E3
• Gene Summary: Suppresses neurite outgrowth.[UniProtKB/Swiss-Prot Function]