Dis3l (NM_172519) Mouse Tagged ORF Clone

CAT#: MG211347

Dis3l (tGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like (Dis3l), transcript variant 2

Product Images

Circular map for MG211347

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dis3l
• Synonyms: AV340375
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211347 representing NM_172519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAACGGCCTGTCAAGCTGTGCAGCACCAGAGAGGCCGGAGACAGTATAACAAACTGCGAAACCTCC
  TGAAGGATGCTCGCCATGACTGCGTTCTCTTTGCTAACGAGTTCCAGCAGCACTGTTACCTCCCCCGGGA
  AAAGGGGGAAGCCATGGAGAAGTGGCAGACCAGGAGCATATACAACTCAGCGGTTTGGTACTATCACCAC
  TGTGAGGACAGGATGCCCATCGTTATGGTGACAGAAGATGAAGAGGCCATTCAGAAGTATGGAAGTGAAA
  CAGAAGGCGTATTTGTCATTTCTTTCAAGAATTACCTGGACAACTTCTGGCCAGATTTGAAGGCTGCCCA
  CGATCTCTGTGACTCCATCCTTCAGTCTCGCCGGGAAAGGGAGACTGAGAGTCAGGAAACCCATGGGAAA
  GAGTACCCAGAACATCTTCCCCTAGAAGTACTGGAAGCGGGCATCAAATCTGGACGCTACATCCAGGGAA
  TTCTGAATGTCAACAAGCACAGAGCTCAGATTGAAGCTTTCGTTCGCCTACACGGAGCCAGCAGTAAGGA
  CTCAGGCTTGGTCAGCGACATCCTCATCCATGGCTCGAAGGCTCGGAACCGCTCCATCCATGGAGACGTC
  GTGGTGGTGGAGATGCTCCCCAAAAGTGAGTGGAAAGGGAGAACAGCCGCCCTGGGTGAGAACGACAGTG
  ATGACAAGGCCTCGGGCGAGTCCCCGAGTGAGCCCATGCCCACAGGTCGAGTGGTAGGCATCCTTCAGAA
  GAACTGGCGAGATTATGTGGTGACATTTCCATCCAAAGAAGAGGTCCAGTCTCAGGGCAAAAATGCTCAG
  AAGATCCTGGTTACGCCGTGGGATTACAGAATCCCTAAGATCCGCATCAGCACCCAGCAAGCAGAAGCCC
  TCCAGGATTTCAGGGTGGTTGTGCGCATTGACTCCTGGGAGGCAACATCAGTGTATCCAAATGGACATTT
  TGTGCGTGTCTTAGGGAGAATCGGTGATCTGGAAGGGGAGATTGCAACCATCCTGGTAGAGAACAGTATC
  TCTGTGGTCCCCTTCTCAGAAGCCCAGATGTGTGAGATGCCAGTGAACACACCAGAAAACCCTTGGAAAG
  TGAGTCCCAAAGAAGAGCAAGAGCGGAAGGACCTGAGGACCACCCACCTCGTGTTCAGCATCGACCCCAA
  AGGTTGTGAAGATGTGGATGACACACTCTCAGTCAGAACCTTGAATAACGGCAACCTGGAGCTGGGGGTC
  CACATCGCTGACGTCACACACTTTGTGGCCCCTAACTCTTACATCGATGTTGAAGCTAGAACGAGGGCCA
  CCACTTACTACCTAGCGGACCGTCGCTATGACATGCTGCCTTCCATCCTCAGCGCAGACCTCTGCTCCCT
  CCTGGGAGGCGTTGACCGGTATGCTGTGTCAGTCATGTGGGAATTAGATAAAACCTCTTATGAAATTAAG
  AAGGTGTGGTACGGCAGAACCATTATCCGATCAGCTTACAAACTGTTCTACGAGGCGGCCCAGGAACTAC
  TGGACGGAAACTTCAGCATTGTTGATGATATTCCAGAACTTAAAGCCTTGGACAAGCAGAGCCAACAGGC
  CAAACTAGAGGAGTTAGTGTGGGCAATTGGAAAGTTGACAGACATAGCTCGCCACATCCGAGCAAAGAGA
  GACCGCTGTGGAGCCTTGGAGCTGGAAGGGGTAGAGGTTCGAGTCCAGCTGGATGACAAGAAGAACATCC
  GTGACCTCATCCCCAAGCAGCCCCTGGAGGTTCACGAGACGGTGGCTGAGTGCATGATCCTAGCCAACCA
  CTGGGTGGCCAAGAAGATCTGGGAGAGCTTCCCCCACCAGGCTCTGCTGCGCCAGCACCCTCCACCACAC
  CAGGAGTTTTTCTCAGAGCTCCGGGAATGTGCTAAAGCAAAAGGCTTCTTCATAGACACACGGTCCAATA
  AAACCCTGGCTGACTCTCTGGATAGTGCAAATGACCCCAAGGACCCCCTGGTAAACAAGCTGCTGCGCTC
  CATGGCCACCCAGGCCATGTCCAACGCGCTCTACTTCTCTACGGGATCCTGCGCAGAGGAGGAGTTCCAT
  CATTACGGGCTGGCCTTAGATAAATACACCCACTTTACCTCTCCAATAAGAAGATACTCAGATATTGTAG
  TACATCGGCTATTAATGGCAGCCATTTCAAAAGACAAGAAAATGGAGATTAAAGAAAATTTGTTCAGCAA
  CAAAAATCTTGAGGAATTATGCAGACACATTAACAACAGAAACCGAGCGGCACAGCGGTCTCAGAAGCAG
  TCCACCGAGCTCTTCCAGTGCATGTACTTTAAAGACCGAGATGCAGAAACTGAGGAGCGCTGCATAGCTG
  ATGGAGTTATTTATTCCATTAGAACAAATGGTGTACTTGTATTTATACCAAGGTTTGGGATTAAAGGTGC
  TGCTTATCTGAAGAATAAAGATAGCTTAGTGATCTCCTGTGGCCCAGAGGGCAGCTCTGAATGGAAGCCA
  GGATCCCTACAAAGATCTCAAAACAAGATCATCTCTACCACAGCTGGAGGGCAGTCTGTTACATTTCATC
  TATTTGACCATGTGACGGTAAGAATTTCTGTCCAGGCCTCGCGCTGCCACTCTGATACAATCAGGCTTGA
  AATAGTAAGCAACAAGCCATACATGATCCCAAACACAGAACTCTGTCACCAGAGCTCCCTGCTGAAGAGT
  GAGTTAGTGAAGGAAGTAACCCGATCTGTGGAGGAAGCGCAGCTTGCACAAGAAGTCAAAGGCAAGGTGA
  TTCAGGAAGAGCATCAAGAATACTGCCAGACAAAGGGAAGAAGTCTGTACACACTTCTGGAGGAGATAAG
  GGACCTAGCTCTTCTGGATGTCTCTGACAGTTGTGCAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211347 representing NM_172519
  Red=Cloning site Green=Tags(s)
  
  MQTACQAVQHQRGRRQYNKLRNLLKDARHDCVLFANEFQQHCYLPREKGEAMEKWQTRSIYNSAVWYYHH
  CEDRMPIVMVTEDEEAIQKYGSETEGVFVISFKNYLDNFWPDLKAAHDLCDSILQSRRERETESQETHGK
  EYPEHLPLEVLEAGIKSGRYIQGILNVNKHRAQIEAFVRLHGASSKDSGLVSDILIHGSKARNRSIHGDV
  VVVEMLPKSEWKGRTAALGENDSDDKASGESPSEPMPTGRVVGILQKNWRDYVVTFPSKEEVQSQGKNAQ
  KILVTPWDYRIPKIRISTQQAEALQDFRVVVRIDSWEATSVYPNGHFVRVLGRIGDLEGEIATILVENSI
  SVVPFSEAQMCEMPVNTPENPWKVSPKEEQERKDLRTTHLVFSIDPKGCEDVDDTLSVRTLNNGNLELGV
  HIADVTHFVAPNSYIDVEARTRATTYYLADRRYDMLPSILSADLCSLLGGVDRYAVSVMWELDKTSYEIK
  KVWYGRTIIRSAYKLFYEAAQELLDGNFSIVDDIPELKALDKQSQQAKLEELVWAIGKLTDIARHIRAKR
  DRCGALELEGVEVRVQLDDKKNIRDLIPKQPLEVHETVAECMILANHWVAKKIWESFPHQALLRQHPPPH
  QEFFSELRECAKAKGFFIDTRSNKTLADSLDSANDPKDPLVNKLLRSMATQAMSNALYFSTGSCAEEEFH
  HYGLALDKYTHFTSPIRRYSDIVVHRLLMAAISKDKKMEIKENLFSNKNLEELCRHINNRNRAAQRSQKQ
  STELFQCMYFKDRDAETEERCIADGVIYSIRTNGVLVFIPRFGIKGAAYLKNKDSLVISCGPEGSSEWKP
  GSLQRSQNKIISTTAGGQSVTFHLFDHVTVRISVQASRCHSDTIRLEIVSNKPYMIPNTELCHQSSLLKS
  ELVKEVTRSVEEAQLAQEVKGKVIQEEHQEYCQTKGRSLYTLLEEIRDLALLDVSDSCAM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172519
• ORF Size: 3476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172519.3, NP_766107.1
• RefSeq Size: 3224 bp
• RefSeq ORF: 2913 bp
• Locus ID: 213550
• UniProt ID: Q8C0S1
• Cytogenetics: 9 C
• Gene Summary: Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA.[UniProtKB/Swiss-Prot Function]