Ap2a2 (NM_007459) Mouse Tagged ORF Clone

CAT#: MG211254

Ap2a2 (tGFP-tagged) - Mouse adaptor protein complex AP-2, alpha 2 subunit (Ap2a2)

Product Images

Circular map for MG211254

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap2a2
• Synonyms: 2410074K14Rik; Adtab; AF006990; AW146353; C78001; L25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211254 representing NM_007459
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCGTATCCAAGGGGGACGGGATGCGAGGCCTAGCGGTCTTCATCTCCGACATCCGCAACTGTA
  AAAGTAAAGAAGCTGAAATAAAGAGAATAAACAAAGAACTGGCAAATATTAGATCCAAATTTAAAGGTGA
  CAACGCTCTTGATGGCTACAGTAAAAAGAAGTATGTCTGCAAATTGCTCTTCATCTTTCTCCTTGGTCAT
  GACATTGACTTTGGACACATGGAAGCTGTGAATCTTCTGAGCTCAAACAGATACACGGAAAAGCAGATTG
  GCTACCTTTTCATCTCTGTATTGGTGAACTCGAATAGTGAACTGATCCGGTTGATTAACAATGCCATCAA
  GAATGACCTGGCCAGCCGCAATCCTACGTTTATGGGTCTCGCCCTGCACTGCATTGCCAATGTGGGTAGC
  CGTGAGATGGCGGAGGCCTTCGCCGGAGAGATCCCCAAGATCCTCGTGGCAGGAGATACCATGGATAGTG
  TGAAGCAGAGCGCAGCCCTGTGCTTGTTACGCCTGTACAGGACATCACCTGACCTAGTTCCCATGGGCGA
  CTGGACATCTCGTGTGGTACACCTCCTCAATGACCAGCACTTGGGGGTAGTGACTGCAGCGACTAGCCTC
  ATCACCACCCTGGCCCAGAAGAACCCTGAGGAGTTCAAGACCTCTGTCTCGCTAGCTGTCTCGCGACTGA
  GCAGGATTGTGACGTCTGCATCAACAGATCTTCAGGATTATACTTACTACTTTGTCCCGGCTCCTTGGCT
  GTCAGTCAAGCTTCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCTGCGGTGCGTGGCCGCCTGACT
  GAGTGCTTGGAGACCATCCTGAACAAGGCCCAGGAGCCACCCAAGTCCAAGAAGGTCCAGCATTCAAATG
  CCAAGAACGCTGTGCTGTTTGAGGCCATCAGCCTGATCATCCACCATGACAGTGAGCCAAACTTGCTCGT
  CCGCGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACGAACCTACGCTACCTGGCTCTGGAG
  AGCATGTGCACGCTGGCCAGCTCTGAGTTCTCCCACGAGGCCGTCAAGACCCACATTGAGACGGTCATCA
  ATGCCTTGAAGACGGAAAGAGACGTGAGTGTGAGGCAGCGGGCAGTGGACCTGCTCTACGCCATGTGTGA
  CCGGAGCAACGCCCAGCAGATTGTGGCTGAGATGCTGAGCTACCTAGAAACGGCTGACTACTCCATCCGA
  GAGGAAATTGTGCTGAAGGTGGCCATCCTGGCTGAGAAGTATGCAGTGGACTACACTTGGTATGTGGACA
  CCATCCTCAACCTCATCCGCATCGCGGGCGACTATGTGAGCGAGGAGGTGTGGTACCGCGTCATCCAGAT
  TGTCATCAATCGCGATGATGTGCAGGGCTATGCTGCCAAGACGGTATTTGAGGCATTGCAGGCGCCAGCA
  TGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGGGACC
  CACGATCCAGCCCTCTGATCCAGTTCAACCTGCTCCACTCCAAGTTCCACTTGTGCAGCGTCCCCACTCG
  GGCACTCCTGCTGTCCACCTACATCAAGTTCGTGAACCTCTTTCCAGAGGTAAAAGCTACCATTCAGGAC
  GTGCTGCGCAGTGACAGCCAGCTGAAGAATGCCGACGTGGAGCTGCAGCAGCGGGCTGTGGAGTACCTGC
  GACTGAGCACTGTCGCCAGCACTGACATCCTGGCAACTGTCCTGGAGGAAATGCCACCCTTCCCTGAGCG
  TGAGTCCTCCATCTTGGCCAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACC
  AAGCGGGAGCGAAGCATCGATGTGAATGGGGGCCCTGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCA
  CACCTTCTCCGTCAGCAGACCTGCTGGGTCTGGGGGCCGTGCCCCCTGCTCCCACGGGGCCCCCTCCCTC
  CTCTGGCGGCGGGCTGCTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGC
  TCTGAAGACAACTTTGCCAGGTTTGTTTGTAAAAATAATGGTGTATTGTTCGAAAACCAGCTGCTTCAAA
  TTGGACTTAAGTCAGAATTTCGGCAGAATTTAGGCCGAATGTTCATATTTTATGGTAACAAGACCTCCAC
  GCAGTTCCTAAACTTCACTCCAACACTAATCTGTGCTGACGACCTTCAAACTAATCTGAACCTGCAGACT
  AAGCCCGTGGACCCAACTGTGGATGGGGGCGCACAGGTGCAACAAGTGGTCAACATTGAGTGTATATCTG
  ATTTCACAGAGGCACCTGTCCTCAACATCCAGTTCAGGTATGGTGGGACCTTCCAGAATGTGTCTGTTAA
  GCTTCCCATCACTCTCAACAAATTTTTCCAGCCCACAGAAATGGCTTCTCAGGATTTCTTTCAACGTTGG
  AAGCAGTTGAGCAATCCACAGCAAGAAGTGCAGAATATCTTCAAAGCAAAGCATCCGATGGACACAGAGA
  TCACTAAGGCAAAGATTATTGGATTTGGTTCTGCGCTCCTGGAAGAAGTTGACCCGAATCCTGCAAATTT
  CGTGGGTGCTGGCATAATACACACCAAAACCACCCAGATTGGCTGCTTGTTGCGCCTGGAGCCAAACCTG
  CAAGCTCAGATGTATAGACTCACCTTGCGTACCAGCAAAGACACCGTCTCTCAGAGACTATGTGAATTGC
  TGTCGGAACAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211254 representing NM_007459
  Red=Cloning site Green=Tags(s)
  
  MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDNALDGYSKKKYVCKLLFIFLLGH
  DIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGS
  REMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSL
  ITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLT
  ECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALE
  SMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIR
  EEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPA
  CHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQD
  VLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEET
  KRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPG
  SEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQT
  KPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRW
  KQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNL
  QAQMYRLTLRTSKDTVSQRLCELLSEQF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007459
• ORF Size: 2814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007459.2, NP_031485.2
• RefSeq Size: 4639 bp
• RefSeq ORF: 2817 bp
• Locus ID: 11772
• UniProt ID: P17427
• Cytogenetics: 7 F5
• Gene Summary: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.[UniProtKB/Swiss-Prot Function]