Fig4 (NM_133999) Mouse Tagged ORF Clone

CAT#: MG211140

Fig4 (tGFP-tagged) - Mouse RIKEN cDNA A530089I17 gene (A530089I17Rik)

Product Images

Circular map for MG211140

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fig4
• Synonyms: A530089I17Rik; AI326867; Sac3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211140 representing NM_133999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCACGGCCGCTGCCCCCATCATCAGCTCGGTCCAGAAGCTGGTGCTGTATGAGACCAGAGCTAGAT
  ACTTTCTAGTTGGGAGCAATCATGCAGAAACGAAGTACCGTGTCCTGAAAATTGATAGAACAGAACCGAA
  GGACTTGGTGGTAATCGACGACAGGCACGTGTACACACAACAAGAAGTGAGGGAACTTCTTGGCCGCCTG
  GATCTGGGAAACAGAACAAAGATGAGCCAGAAAGGATCCTCCGGGTTGTTTCGAGCCGTCTCAGCTTTTG
  GAGTCGTAGGTTTTGTCAGGTTCTTAGAAGGCTACTATATTGTATTAATAACTAAGCGGAGGAAGATGGC
  AGACATTGGAGGTCATGCAATTTATAAGATTGAAGATACAAGTATGATTTATATCCCCAATGACTCTGTT
  CGGATTTCTCACCCCGACGAAGCTAGGTATCTAAGGATATTTCAAAATGTGGATCTATCTAGCAATTTTT
  ACTTTAGTTACAGCTATGATTTGTCACACTCACTTCAGTATAATCTTACCGTCCTGCGAATGCCCCTGGA
  GATGTTAAAGTCAGAAACATCCAAGGCGTGCCAGGAGAGCTTCGACATCTTTGAAGATGAAGGATTAATT
  ACACAGGGTGGCAGTGGTGTATTTGGGATCTCTAGTGAGCCTTATATGAAGTATGTATGGAACGGTGAAC
  TTCTGGATATAATTAAAAACACTGTGCATCGTGACTGGCTATTGTATATCATTCATGGGTTCTGCGGGCA
  GTCCAAGCTTTTGATCTATGGACGACCAGTGTATGTGACCCTAATAGCCAGGAGATCCAGTAGGTTTGCT
  GGGACGCGCTTTCTCAAGAGAGGCGCAAACTGTGAGGGTGACGTTGCAAATGAGGTGGAGACCGAGCAGA
  TCCTCTGTGATGCTTCTGTGATGTCTTTTACCGCAGGCAGTTACTCTTCTTACGTACAAGTTAGAGGATC
  CGTTCCCTTATTCTGGTCCCAAGACATCTCAACTATGATGCCGAAACCACCCATTACACTGGACCAGGCG
  GATCCCTTTGCACACGTGGCTGCTCTTCACTTTGACCAGATGCTCCAGAGGTTCGGCTCCCCCATCATCA
  TCTTGAACTTAGTGAAGGAGAGGGAGAAGAGAAAGCACGAAAGGATCCTGAGTGAAGAGCTGGTGGCTGC
  GGTCACTTACCTGAACCAGTTCCTGCCACCGGAGCACACCATTGTCTACATCCCCTGGGACATGGCCAAG
  TACACCAAGAGTAAGCTGTGCAACGTTCTCGATCGGCTGAATGTGATTGCGGAAAGCGTGGTAAAGAAGA
  CAGGATTCTTTGTAAACCGCCCTGATTCTTACTGCAGCATTTTACGGCCAGATGAAAAGTGGAATGAACT
  AGGAGGTCATGTGATTCCCACTGGTCGGCTCCAGACTGGCATCCTTCGAACCAACTGTGTGGACTGTTTG
  GATCGCACCAACACTGCGCAGTTCATGGTGGGCAAGTGTGCCCTGGCTTACCAGCTGTATTCCTTAGGGC
  TGATTGACAAGCCTAACCTGCAGTTCGACACGGATGCAGTGAGGTTGTTTGAGGAACTCTATGAAGACCA
  TGGAGACACCCTTTCCCTGCAGTACGGAGGCTCTCAGCTGGTTCATCGGGTAAAGACCTACAGAAAGATA
  GCACCGTGGACCCAGCACTCCAAGGACATCATGCAGACTCTGTCCCGGTACTACAGCAACGCCTTCTCGG
  ATGCTGATAGACAAGATTCCATCAACCTGTTTTTGGGTGTTTTCCACCCCACAGAAGGGAAACCTCATCT
  CTGGGAACTCCCCACAGACTTTTACCTCCATCACAAAAACACCATGAGCCTTTTACCCCCCAGAAGGAGT
  TATACTTACTGGTGGACGCCAGAGGTGGTGAAGCATCTGCCGCTGCCTTACGATGAAGTTATCTGTGCTG
  CAAACTTAAAGAAGTTGATGGTGAAGAAATTTCACAGATGGGAAGAAGAGATTGATATTCACAATGAGTT
  CTTCCGGCCGTATGAATTGAGTAGTTTTGATGACACCTTTTGCTTGGCCATGACAAGTTCAGCACGTGAT
  TTTATGCCTAAAACTGTTGGCATTGATCCAAGTCCATTTACCGTGCGAAAACCAGATGAAACTGGAAAAT
  CTGTACTGGGGAACAAAAACACTAGAGAGGAAGCTGTCCTGCAGCGGAAGACGGCAGCCAGCGCCCCGCC
  ACCCCCGAGCGAGGAGGCTGTGTCCAGCAGCTCTGAGGATGACTCTGGCACCGACCGGGAAGATGAAGGC
  TCCATCTCTCAGCGATCCACTCCTGTGAAGATGACTGACACGGGAGACAGTGCCAAAGCGACTGAGAATG
  TGGTCCAGCCCATGAAGGAAGTGTATGGAGTCAGTCTCTCCAGTAGCCTGTCAGAGGAGGATCACTCCAT
  TTATGCCAGGTTTGTTCAGCTGGGGCAAAGTCAACATAAACAGGACCGAGGTAACCAGCAGCTGTGTTCC
  CGTTGCTCAGATGGAGTTATAAAACTAACTCCCATCTCAGCTTTCTCGCAAGACAACATTTATGAAGTTC
  AGCCTCCAAGAGTAGACAGAAAGTCTACAGAGATCTTCCAGGCCCATATCCAAGCTAGCCAAGGTATCAT
  GCAGCCCCTTGGCAAAGAGGACACTGCCATGTACCGGGAGTACATCAGAAACCGCTACTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211140 representing NM_133999
  Red=Cloning site Green=Tags(s)
  
  MPTAAAPIISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRL
  DLGNRTKMSQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKIEDTSMIYIPNDSV
  RISHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETSKACQESFDIFEDEGLI
  TQGGSGVFGISSEPYMKYVWNGELLDIIKNTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFA
  GTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLFWSQDISTMMPKPPITLDQA
  DPFAHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAK
  YTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGHVIPTGRLQTGILRTNCVDCL
  DRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKI
  APWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMSLLPPRRS
  YTYWWTPEVVKHLPLPYDEVICAANLKKLMVKKFHRWEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARD
  FMPKTVGIDPSPFTVRKPDETGKSVLGNKNTREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREDEG
  SISQRSTPVKMTDTGDSAKATENVVQPMKEVYGVSLSSSLSEEDHSIYARFVQLGQSQHKQDRGNQQLCS
  RCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDTAMYREYIRNRYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133999
• ORF Size: 2721 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133999.1, NP_598760.1
• RefSeq Size: 3278 bp
• RefSeq ORF: 2724 bp
• Locus ID: 103199
• UniProt ID: Q91WF7
• Cytogenetics: 10 B1
• Gene Summary: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.[UniProtKB/Swiss-Prot Function]