Atp6v0a2 (NM_011596) Mouse Tagged ORF Clone

CAT#: MG210967

Atp6v0a2 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A2 (Atp6v0a2)

Product Images

Circular map for MG210967

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp6v0a2
• Synonyms: 8430408C20Rik; AI385560; ATP; ATP6a2; Atp6n1d; Atp6n2; AW489264; C76904; ISF; J6B7; SHIF; Stv1; Tj6; TJ6M; TJ6s; V-ATPase 116 kDa; V-ATPase a2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210967 representing NM_011596
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCTCTCTTCCGCAGCGAGAGCATGTGCCTGGCGCAGCTCTTCCTGCAGTCGGGCACGGCCTACG
  AGTGTCTGAGCGCGCTGGGCGAGAAGGGCCTGGTGCAGTTCCGAGACCTCAATCAAAATGTAAGTTCTTT
  TCAAAGAAAATTCGTCGGTGAGGTAAAGAGGTGTGAAGAGCTCGAACGAATACTGGTGTACCTGGTGCAG
  GAAATCACCAGAGCTGATATTCCCCTGCCTGAAGGAGAGGCCAGTCCTCCCGCACCACCTCTTAAACATG
  TCCTAGAAATGCAGGAACAGCTGCAGAAGCTGGAGGTGGAGCTCAGAGAAGTCACCAAGAACAAGGAGAA
  GCTGAGGAAGAACCTGTTGGAGCTGGTGGAGTACACCCACATGCTGAGGGTCACGAAGACCTTCCTCAAG
  CGGAATGTGGAGTTTGAGCCCACGTATGAAGAGTTTCCCGCCTTAGAGAACGACTCTCTGTTGGACTACA
  GCTGCATGCAGCGGCTGGGCGCCAAGCTGGGATTCGTTTCTGGCCTGATTCAGCAAGGCAGAGTTGAAGC
  ATTTGAAAGGATGCTGTGGCGGGCCTGCAAAGGGTACACCATTGTGACCTATGCAGAGCTGGACGAGTGC
  CTGGAGGACCCTGAAACGGGTGAAGTCATAAAGTGGTACGTGTTCCTGATTTCCTTTTGGGGAGAGCAGA
  TTGGCCACAAGGTTAAGAAGATATGTGACTGTTACCACTGCCACATCTACCCGTATCCAAACACGGCTGA
  GGAGCGCAGGGAGATTCAGGAGGGGCTCAATACTCGGATCCAAGACCTTTACACGGTGCTGCACAAAACG
  GAGGACTATCTGCGGCAAGTGCTCTGCAAAGCTGCCGAGTCCGTGTGCAGCCGTGTGGTCCAGGTGAGGA
  AGATGAAGGCCATCTACCACATGCTCAACATGTGCAGCTTTGACGTCACCAACAAGTGCCTCATAGCTGA
  GGTCTGGTGCCCCGAGGTGGACCTGCCAGGCTTGCGCAGAGCACTGGAGGAAGGCTCGAGAGAGAGCGGA
  GCTACAATCCCCTCGTTCATGAACACAATCCCTACGAAAGAAACACCCCCCACTCTGATCCGCACCAACA
  AATTCACTGAAGGTTTCCAGAACATCGTGGATGCCTATGGAGTCGGGAGCTACAGAGAAGTGAACCCAGC
  TCTCTTTACCATCATCACCTTCCCGTTCCTGTTTGCTGTGATGTTTGGCGACTTTGGGCACGGCTTTGTC
  ATGTTCCTGTTTGCCCTCTTACTGGTGTTAAATGAGAATCACCCCAGACTAAGCCAGTCACAGGAGATCC
  TTAGGATGTTCTTTGACGGCCGGTATATCCTGCTGCTGATGGGGCTGTTCTCTGTGTACACCGGCCTCAT
  CTACAACGACTGCTTCTCCAAGTCTGTGAACCTCTTTGGTTCTGGGTGGAACGTATCTGCCATGTACAGC
  TCCAGCCACTCTCCAGAGGAGCAGAGGAAGATGGTACTTTGGAATGACAGCACCATCAGGCACAGCAGGA
  CTTTGCAGCTGGACCCGAATATCCCTGGAGTTTTCCGAGGCCCCTACCCTTTTGGCATTGATCCTATTTG
  GAACCTGGCCACAAACCGCCTCACGTTCCTCAACTCCTTCAAGATGAAAATGTCTGTGATTTTAGGAATT
  TTTCACATGACTTTTGGTGTTGTTCTGGGAATATTTAACCACTTGCACTTTAGGAAGAAGTTCAACGTCT
  ACCTGGTCTCGGTGCCTGAGATCCTGTTCATGCTCTGCATCTTTGGGTACCTGATTTTCATGATCATTTA
  CAAGTGGCTGGCATACTCGGCAGAGACCTCGAGAGAGGCCCCGAGCATCCTGATCGAGTTCATTAACATG
  TTCCTGTTCCCAACCAGCAAGACACACGGGCTCTACCCAGGGCAGGCACACGTCCAGAGAGTGTTGGTGG
  CTCTCACGGTGCTGGCTGTCCCCGTGCTCTTCTTAGGAAAGCCGCTTTTTCTGCTGTGGCTGCACAACGG
  GCGCAATTGCTTTGGCATGAGCCGGAGCGGTTACACACTCGTGAGGAAGGACAGCGAGGAAGAGGTGTCT
  CTTCTGGGCAACCAGGACATAGAAGAGGGCAACAGCCGCATGGAAGAAGGCTGCCGAGAAGTGACGTGTG
  AGGAGTTTAACTTCGGGGAGATCCTGATGACGCAGGCCATCCACTCCATTGAGTACTGCCTCGGCTGCAT
  CTCCAACACCGCCTCCTACCTGAGGCTCTGGGCACTCAGCCTGGCCCATGCACAGCTCTCTGATGTGCTG
  TGGGCCATGCTGATGCGCGTGGGGCTGCGTGTGGACACCACCTATGGGGTCTTGCTGCTGCTGCCTGTCA
  TGGCTTTCTTTGCAGTTTTGACCATTTTTATCCTTCTGGTCATGGAGGGTCTTTCTGCATTCCTCCACGC
  CATAAGGCTTCACTGGGTAGAATTTCAGAACAAATTCTACGTTGGTGCAGGCACCAAGTTTGTTCCCTTC
  TCCTTCAGTCTGCTCTCCTCCAAGTTCAGTAATGATGACAGCATAGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210967 representing NM_011596
  Red=Cloning site Green=Tags(s)
  
  MGSLFRSESMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQ
  EITRADIPLPEGEASPPAPPLKHVLEMQEQLQKLEVELREVTKNKEKLRKNLLELVEYTHMLRVTKTFLK
  RNVEFEPTYEEFPALENDSLLDYSCMQRLGAKLGFVSGLIQQGRVEAFERMLWRACKGYTIVTYAELDEC
  LEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHIYPYPNTAEERREIQEGLNTRIQDLYTVLHKT
  EDYLRQVLCKAAESVCSRVVQVRKMKAIYHMLNMCSFDVTNKCLIAEVWCPEVDLPGLRRALEEGSRESG
  ATIPSFMNTIPTKETPPTLIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFV
  MFLFALLLVLNENHPRLSQSQEILRMFFDGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYS
  SSHSPEEQRKMVLWNDSTIRHSRTLQLDPNIPGVFRGPYPFGIDPIWNLATNRLTFLNSFKMKMSVILGI
  FHMTFGVVLGIFNHLHFRKKFNVYLVSVPEILFMLCIFGYLIFMIIYKWLAYSAETSREAPSILIEFINM
  FLFPTSKTHGLYPGQAHVQRVLVALTVLAVPVLFLGKPLFLLWLHNGRNCFGMSRSGYTLVRKDSEEEVS
  LLGNQDIEEGNSRMEEGCREVTCEEFNFGEILMTQAIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVL
  WAMLMRVGLRVDTTYGVLLLLPVMAFFAVLTIFILLVMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPF
  SFSLLSSKFSNDDSIA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011596
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011596.5, NP_035726.2
• RefSeq Size: 3037 bp
• RefSeq ORF: 2571 bp
• Locus ID: 21871
• UniProt ID: P15920
• Cytogenetics: 5 F
• Gene Summary: This gene encodes a subunit of vacuolar ATPase, a multimeric enzyme that localizes to intracellular vesicles and to the plasma membrane of specialized cells. The encoded protein is a component of the V(0) domain, which functions in proton translocation across membranes. Function of this gene is important in fetal-specific immune suppression during pregnancy. [provided by RefSeq, May 2013]