Fam13b (NM_146084) Mouse Tagged ORF Clone

CAT#: MG210952

Fam13b (tGFP-tagged) - Mouse RIKEN cDNA 2610024E20 gene (2610024E20Rik)

Product Images

Circular map for MG210952

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam13b
• Synonyms: 2610024E20Rik; AW060714; AW546153
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210952 representing NM_146084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGAGCTCCTCCCCTTCCTTGAGTAACTGCAACTCCGATCTTGCTAGCAAAATATTTGGAATTC
  CACTTGATGAGCTGCAGCAGGGAGGACATCCAGACAATGAGGTTCCGTTCATAGTCCGCCACGTTGTGGA
  CTATATTGAGGAACATGGAGGTCTGGAGCAACAAGGGCTGTTTCAAGTCAATGGGAATGCTGAGACAGTG
  GAGTGGCTTCGGCAGAGATACGACAGTGGAGAAGAGGTGGACTTGGTTAAGGAAGCGGACGTCCCCTCAG
  CCATTAGTCTCCTTAGGTTTTTCCTGCAGGAGCTCCCTGAGCCCGTCATACCTGGCAGCTTGCATATTCA
  CTTGCTGCAGCTTTCTCAAGATTATAATAATGAAGATGAATTTGGAAGAAAGTTGAGGTTCCTCTTGCAA
  CAGCTTCCACCTGTTAATTACAGTTTGTTAAAGTTTCTGTGTAGGTTTTTAGCCAATGTAGCATCACATC
  ATGAAGAAATTTGGTCTGCAAATTCTTTGGCTGCTGTTTTTGGTCCAGATGTCTTCCACATTTACACGGA
  TGTGGAAGATATGAAAGAGCAGGAAATTGTGAGCAGGATAATGGCGGGACTTCTAGAAAATTATTACGAG
  TTTTTTGAGAATGAAGAGGAAGATTTTTCATCAAATGATTTGAGTTCAATTACTGAGCAGGTTAATGAAC
  TTTCTGAGGAAGAAGAGGAAGATGAAAAGCTGGAGCATATAGAAGAACTTCCAGAAGAAGGTGTAGAAAA
  GTCAGCTGGCATGCCAGAGGTGCTGCAGTTAAGGATGACTGAAAACCTTCTGGACTCAGACAGTGTTACA
  GCATCAACAAGGATTGATGCTGCTGCTGCTACTACTACTAATGCCAGTGATGGTAACATTAAGTGTTCAA
  AACCTGTGGCTGGTACTACTGCAGATAATGAAGTCATGCAGCAAGATTTTGTATTTGAGGATCAGAAAAA
  TAATGAGTCTGTAGGTATACTGTTAGAGCCTTGTAGTGACCACGGGGATAGTGAAGATGGCTGTCCTGAG
  AGGAAGGAATATTTATTATGTGACAGTGATAAATTGCCGCACTTGATTCTGGATTCTAGTAGCAAGATAC
  GTGATTTGAATGCCAACACTGAATTGGAAGTAACGGAAGGTCAGAGTGTTGGTGTTCAAGGAGAAGCAGC
  GTGTATACAGATTGCGCAGTTAGATCTGAAGAACGTTTCTGATGGTGATAAATGGGAAGAGCCGTTTCCT
  GCATTTAAGTCTTGGCAGGAAGACTGTGAGTCTGGAGAAGCTCAGCTGTCTCCACAGGCTGCCAGGATGA
  CTCACCACCCCCTGGGAGAGGACTGCCCTCCAGTATTATCTCATCGTAGTTTAGATTTTGGGCAAAGCCA
  ACGTTTCCTACATGATCCAGAAGCGTTGGATTTCTCGTCAAAGGCACTTTCCTTCACTAGAATTCGAAGG
  TCATCCTTCAGTTCAAAAGATGAGAAAAGAGAAGACAGAACACCATATCAGTTGGTCAAGAAACTTCAGA
  AAAAAATCAGACAATTCGAGGAGCAATTTGAAAGGGAAAGAAATAGCAAGCCCTCCTACAGCGACATTGC
  AGCCAATCCGAAGGTATTAAAATGGATGACAGAGCTTACGAAATTGCGGAAGCAAATTAAAGATGCAAAA
  CACAAAAACTCTGATGGAGAATTTGCGCCTCAGACACGTCCTCGGAGTAACACTCTGCCAAAAAGTTTTG
  GCTCTTCTCTAGACCATGAGGACGGCGAGAGTGAAGGTGAGCCCAGAGTCATTCAGAAAGAGAAGACGCC
  ATCGAAAGAAGCAACTCTTGAGCTGATTACCAAACGGTTGAAGGAGAACCGTGCCGAGCGGCACCTTCCT
  GAGGATATCAAGAAAATGACAAAAGATCATTTGATAGAAGAGAAAACGTCTCTGCAGAAAAGTCTGCTTT
  ACTATGAAAGTCAACACGGAAGGCCGGTGACCAGGGAAGAAAGGCACATTGTTAAGCCTCTCTATGATAG
  ATACAGGCTTGTAAAACAGATGCTGACAAGAGCTAGCATTACTCCTGTCCTTGGTTCTCCTTCCACGAAG
  CGCCGGGGTCAGATGTTACAGCCAATCATAGAAGGAGAAACGGCACACTTTTTTGAAGAAATCAAGGAAG
  AAGAAGAAGATGGTGTCAGCCTCTCCTCTGAGTTAGGTGACATCTTGAGCACAAGCGTGCACACACAGTC
  GTCCTTGGAAAACTTAGAATCTGATGCTGAAGAAAATCAAGAAAAACTGGCTCGGGATCTCTGCTTATCA
  AGTACCCGGGCAGCTTCTGTGCCTGAATTACTGGAACAACTTTGGAAAGCCAGAGCTGAGAAAAAGAAAC
  TACGTAAGATGCTACGGGAGTTTGAAGAAGCGTTCTATCAACAAAATGGAAGGAATGCCCAGAAGGAGGA
  TCGTGTTCCGGTGCTTGAGGAATATAAGGAATACAAGAGAATCAAAGCCAAGCTTAGACTTCTTGAAGTT
  CTTATCAGCAAACAAGACTCTTCAAAATCCATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210952 representing NM_146084
  Red=Cloning site Green=Tags(s)
  
  MRKSSSPSLSNCNSDLASKIFGIPLDELQQGGHPDNEVPFIVRHVVDYIEEHGGLEQQGLFQVNGNAETV
  EWLRQRYDSGEEVDLVKEADVPSAISLLRFFLQELPEPVIPGSLHIHLLQLSQDYNNEDEFGRKLRFLLQ
  QLPPVNYSLLKFLCRFLANVASHHEEIWSANSLAAVFGPDVFHIYTDVEDMKEQEIVSRIMAGLLENYYE
  FFENEEEDFSSNDLSSITEQVNELSEEEEEDEKLEHIEELPEEGVEKSAGMPEVLQLRMTENLLDSDSVT
  ASTRIDAAAATTTNASDGNIKCSKPVAGTTADNEVMQQDFVFEDQKNNESVGILLEPCSDHGDSEDGCPE
  RKEYLLCDSDKLPHLILDSSSKIRDLNANTELEVTEGQSVGVQGEAACIQIAQLDLKNVSDGDKWEEPFP
  AFKSWQEDCESGEAQLSPQAARMTHHPLGEDCPPVLSHRSLDFGQSQRFLHDPEALDFSSKALSFTRIRR
  SSFSSKDEKREDRTPYQLVKKLQKKIRQFEEQFERERNSKPSYSDIAANPKVLKWMTELTKLRKQIKDAK
  HKNSDGEFAPQTRPRSNTLPKSFGSSLDHEDGESEGEPRVIQKEKTPSKEATLELITKRLKENRAERHLP
  EDIKKMTKDHLIEEKTSLQKSLLYYESQHGRPVTREERHIVKPLYDRYRLVKQMLTRASITPVLGSPSTK
  RRGQMLQPIIEGETAHFFEEIKEEEEDGVSLSSELGDILSTSVHTQSSLENLESDAEENQEKLARDLCLS
  STRAASVPELLEQLWKARAEKKKLRKMLREFEEAFYQQNGRNAQKEDRVPVLEEYKEYKRIKAKLRLLEV
  LISKQDSSKSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146084
• ORF Size: 2553 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146084.1, NP_666196.1
• RefSeq Size: 3359 bp
• RefSeq ORF: 2556 bp
• Locus ID: 225358
• UniProt ID: Q8K2H3
• Cytogenetics: 18 B1