Ftsj3 (BC012281) Mouse Tagged ORF Clone

CAT#: MG210907

Ftsj3 (tGFP-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961)

Product Images

Circular map for MG210907

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ftsj3
• Synonyms: AA537063; AU045295; C79843; D11Ertd400e; Epcs3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210907 representing BC012281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAAGGCAAAGTAGGCAAGAGCCGGCGGGACAAGTTCTATCACTTGGCCAAGGAGACTGGTT
  ATCGTTCGCGCTCTGCCTTCAAACTGATCCAGCTCAATCGCCGCTTTCAGTTCCTGCAGAAAGCCCGGGC
  CTTGTTGGACCTGTGTGCTGCGCCTGGCGGATGGCTGCAGGTGGCTGCCAAGTTTATGCCTGTATCCAGT
  CTTATTGTAGGAGTGGACCTCGTTCCAATCAAGCCTCTCCCCAATGTGGTGACTCTCCAGGAGGATATCA
  CAACAGAACGCTGTAGGCAGGCCCTGAGGAAGGAGCTGAAGACCTGGAAAGTTGATGTTGTGCTTAATGA
  TGGGGCTCCCAATGTGGGGGCTAGTTGGGTCCATGACGCTTACTCCCAAGCTCACCTAACTCTGATGGCT
  CTGCGTTTGGCTTGTGATTTTTTGGCCCGTGGTGGTTGTTTCATCACAAAAGTCTTTCGTTCCCGAGACT
  ATCAGCCCTTGCTGTGGATCTTCCAGCAGCTGTTCCACCGTGTCCAGGCCACGAAGCCCCAAGCCTCTCG
  CCATGAATCTGCAGAAATCTTTGTAGTCTGTCAAGGATTCCTGGCTCCGGACAAGGTTGATGCTAAATTC
  TTTGATCCCAAGTTTGCCTTTAAGGAGGTTGAAGTTCAGGCTAAAACTGTTACTGAGTTGGTGACAAGGA
  AGAAGCCAAAGGCTGAAGGCTATGCCGAAGGTGACCTTACTCTTTATCACCGAACCTCAGTTACGGACTT
  CTTGCGAGCTGCCAATCCTGTTGACTTCCTCTCCAAGGCCAGCGAAATCTCAATAGATGATGAAGAGTTG
  GCACAGCACCCAGCTACAACTGAAGACATTAGAGTATGCTGTCAGGACATCAAAGTGCTGGGGCGAAAGG
  AACTTAGGTCTCTTCTGAATTGGAGAACAAAGCTTCGGCGATATGTGGCTAAGAAGCTGAAAGAGCAAGC
  AAAGGCATTGGACATCAGCCTCAGCTCAGAAGAAGAAGAAGAAGGTGATGAAGAAGAGGCAGTAGCTGAG
  ACAAAGCAGGCGCCAGAGGAAGAAGAAGAGAGAGAGGAAGAACAGCTTAATCGGACTCTGGCAGAGATGA
  AAGCCCAAGAAGTGGCAGAACTCAAGAGGAAGAAGAAGAAGTTGCTTCGTGAGCAGAGGAAGCAGCGGGA
  GCGTGTCGAGCTGAAGATGGATTTGCCTGGGGTTTCTATTGCAGATGAGGGGGAGACAGGCATGTTCTCC
  CTGCGAACCATCCGTGGCCAGCAGCTATTAGAGGAAGTAACACAAGGAGACATGAACGCTGCAGACACCT
  TTCTGTCTGATCTGCCAAGGGATGACATCTATGTGTCAGATGCTGAGGACGATGATGATACTTCTCTGGA
  AAGTGACCTGGATCCAGAAGAGCTGGCAGGAGTCAGGACACACTCGGATCTGAAGGAGCAGAAGTACTTG
  CGGTTTACTAAAGTAGATGACAATAAAGAGGAAGGAGGAGAAAATCCATTGCTGGTACCACTGGAAGAAA
  AGGCAGTACTGCAGGAAGAGCAAGCTAGCCTGTGGTTCTCCAAGGACGGCTTCAGTGGGATAGAGGATGA
  TGCTGACGAAGCCCTGGAGATCAGTCAGGCACAGCTGCTGTACAAGAGCCGTCGGAAGGAGCAGCAGCCA
  ACAGATCCACCTCCACCACCTACTAACTTGAAGACTGAGAAGAAATCTCCCCAGGGCCAGAATGAGGTCC
  CTAAGGAGACAGAAGCCATCTTGGGGACAGAGGCTGTCACTGACCCTGGAGGGGAGGAAAGAGGCAACAG
  CTCAGATAGTGACAGCAGCAGTAGTGAGGATGAAGATAGCTGGAAAGTGTCCCGTGGTGTGAAGCGAGGC
  CGTGGCTCTAAGGCAGATGAGGATGGGTTTGAGGTGGTGCCTATTCAGGACCCAGTGAAATACCGAATTC
  TGGACCCTGAAGGCCTTGCTCTAGGTGCTGTTATTGCCTCTTCAAAAAAGGCAAAGAGAGACCTCATAGA
  TAACTCGTTCAACCGGTATGCTTTTAATGAAGAGGAGGGAGAACTTCCTGAGTGGTTTGCACAGGAAGAA
  AAGCAGCATCGGATACGCCAGCTGCCTGTTGATAAGAAGGAGGTGGAACATTACCGAAAACGCTGGCGGG
  AAATCAATGCACGGCCCATCAAGAAAGTAGCTGAGGCCAAGGCCAGAAAGAAACGGAGGATGCTGAAGAA
  GCTGGAACAGACCAAAAAGAAGGCAGAAGCTGTGGTGAATACAGTGGACATCTCAGAACGGGAAAAGGTG
  GCACAGCTGGCCAGTCCAGTACATACTGCTGGACTTGGCATAGCAGTCATTGTTAGAGTTTTTCATCTCG
  CCTTTAAGGGTGTGAACCACAATAATCTCCCAGGATGCAAGCCAAAAGGTCACGCAAAGATAGTTGTTGC
  TCGAATGAAGAAGGATCAGAGAGCACAACAACGGAAGGAACAGAAGAAAAAACACAAGCGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210907 representing BC012281
  Red=Cloning site Green=Tags(s)
  
  MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSS
  LIVGVDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMA
  LRLACDFLARGGCFITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGFLAPDKVDAKF
  FDPKFAFKEVEVQAKTVTELVTRKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISIDDEEL
  AQHPATTEDIRVCCQDIKVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSEEEEEGDEEEAVAE
  TKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFS
  LRTIRGQQLLEEVTQGDMNAADTFLSDLPRDDIYVSDAEDDDDTSLESDLDPEELAGVRTHSDLKEQKYL
  RFTKVDDNKEEGGENPLLVPLEEKAVLQEEQASLWFSKDGFSGIEDDADEALEISQAQLLYKSRRKEQQP
  TDPPPPPTNLKTEKKSPQGQNEVPKETEAILGTEAVTDPGGEERGNSSDSDSSSSEDEDSWKVSRGVKRG
  RGSKADEDGFEVVPIQDPVKYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGELPEWFAQEE
  KQHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKKLEQTKKKAEAVVNTVDISEREKV
  AQLASPVHTAGLGIAVIVRVFHLAFKGVNHNNLPGCKPKGHAKIVVARMKKDQRAQQRKEQKKKHKRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC012281
• ORF Size: 2516 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012281, AAH12281
• RefSeq Size: 2883 bp
• RefSeq ORF: 2516 bp
• Locus ID: 56095
• Cytogenetics: 11 68.89 cM
• Gene Summary: RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.[UniProtKB/Swiss-Prot Function]