Kat2b (NM_020005) Mouse Tagged ORF Clone

CAT#: MG210784

Kat2b (tGFP-tagged) - Mouse p300/CBP-associated factor (Pcaf)

Product Images

Circular map for MG210784

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kat2b
• Synonyms: A930006P13Rik; AI461839; AW536563; p/CAF; Pcaf
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210784 representing NM_020005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGCTGGCGGGGCCGGGTCCCCAGCACTGCCTCCCGCGCCCCCGCACGGTTCCCCCCGGACCC
  TGGCCACCGCTGCCGGGAGCTCTGCTTCCTGCGGGCCAGCGACCGCGGTGGCCGCGGCGGGCACGGCCGA
  GGGACCGGGAGGAGGCGGCTCGGCCCGGATCGCCGTGAAGAAGGCGCAGTTGCGCTCTGCTCCGCGGGCG
  AAGAAGCTGGAGAAACTCGGCGTGTACTCCGCCTGCAAGGCAGAGGAGTCCTGTAAATGCAATGGCTGGA
  AGAACCCTAACCCCTCTCCTACTCCACCAAGAGGAGACCTCCAGCAGATAATTGTCAGTTTGACAGAATC
  CTGTCGAAGCTGTAGCCATGCCCTTGCTGCTCACGTTTCTCACTTGGAGAATGTGTCAGAGGAAGAGATG
  GACAGACTCCTGGGAATTGTGTTGGATGTGGAGTACCTCTTCACCTGCGTCCACAAAGAAGAAGATGCAG
  ATACCAAACAAGTGTACTTCTACCTATTCAAGCTCTTGAGAAAGTCAATTTTACAAAGAGGAAAACCTGT
  GGTTGAAGGCTCCTTGGAGAAGAAGCCGCCATTTGAGAAGCCCAGTATTGAACAGGGTGTGAACAACTTC
  GTGCAGTACAAGTTTAGTCACTTGCCATCGAAAGAGAGGCAGACAACGATCGAGCTGGCCAAGATGTTTC
  TGAACCGCATCAACTACTGGCATCTGGAGGCTCCATCTCAGCGGAGACTACGGTCTCCCAATGATGACAT
  CTCTGGATACAAGGAAAACTACACAAGGTGGTTGTGCTACTGCAATGTACCGCAGTTCTGTGACAGCTTA
  CCTCGGTACGAAACCACAAAGGTGTTTGGGAGAACATTGCTTCGCTCGGTCTTCACCATCATGAGACGAC
  AGCTCTTGGAACAAGCCAGACAGGAAAAAGACAAACTGCCTCTTGAGAAACGCACGCTTATCCTCACACA
  TTTCCCAAAGTTTCTGTCCATGTTGGAAGAAGAAGTGTATAGTCAAAATTCTCCTATCTGGGATCAGGAT
  TTTCTCTCAGCCTCTTCCAGAACCAGCCCGCTAGGAATCCAAACAGTAATCAGTCCTCCTGTTACTGGGA
  CAGCATTGTTCAGTTCAAATTCCACTTCCCATGAGCAGATCAATGGAGGGAGAACAAGTCCTGGATGCAG
  AGGCTCTTCTGGGCTTGAAGCAAACCCGGGAGAAAAGAGGAAAATGAACAACTCTCATGCTCCCGAGGAG
  GCCAAGAGATCTCGAGTGATGGGGGATATTCCCGTGGAATTGATCAATGAGGTCATGTCTACCATCACAG
  ACCCTGCAGGGATGCTTGGACCAGAGACCAATTTTCTGTCAGCCCATTCGGCCAGAGATGAGGCGGCACG
  GCTGGAAGAACGCAGGGGTGTCATTGAATTCCACGTGGTGGGCAACTCCCTGAACCAGAAACCAAACAAG
  AAGATCCTGATGTGGCTCGTGGGCCTCCAGAATGTGTTTTCCCACCAGCTGCCCAGAATGCCCAAAGAGT
  ACATCACACGGCTCGTCTTTGACCCGAAACACAAAACCCTTGCTTTAATTAAAGATGGCCGTGTCATTGG
  TGGTATCTGTTTCCGGATGTTTCCATCCCAGGGATTCACAGAGATTGTTTTCTGTGCAGTAACCTCAAAT
  GAACAAGTCAAGGGCTATGGAACCCACCTGATGAACCATCTCAAAGAATACCACATAAAGCACGAGATCC
  TCAACTTCCTCACATATGCAGATGAGTATGCCATCGGCTATTTCAAGAAGCAGGGTTTCTCCAAAGAAAT
  CAAAATACCTAAAACCAAATATGTTGGCTACATCAAGGATTATGAAGGGGCCACTTTGATGGGATGTGAG
  CTGAACCCTCAGATCCCATACACAGAGTTCTCTGTCATCATTAAAAAGCAGAAGGAGATCATTAAAAAGC
  TGATAGAAAGAAAACAAGCCCAGATTCGAAAAGTCTACCCTGGACTTTCGTGTTTCAAAGATGGAGTTCG
  GCAGATTCCTATAGAAAGCATTCCTGGAATCAGAGAGACAGGCTGGAAACCAAGTGGAAAAGAGAAAAGT
  AAAGAGCCCAAAGACCCTGAGCAGCTTTACAGCACCCTCAAGAACATCCTGCAGCAGGTGAAGAACCATC
  CAAATGCTTGGCCTTTCATGGAACCAGTGAAGAGAACAGAAGCTCCGGGATATTATGAAGTTATAAGGTT
  CCCCATGGATCTGAAAACCATGAGTGAACGCCTCAGGAACAGGTACTATGTGTCTAAGAAGTTATTCATG
  GCGGACTTGCAACGAGTGTTCACCAACTGCAAGGAGTACAACCCTCCCGAGAGCGAGTACTACAAATGCG
  CCAGCATCCTGGAGAAGTTCTTCTTCAGTAAAATTAAGGAAGCAGGGTTGATTGACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210784 representing NM_020005
  Red=Cloning site Green=Tags(s)
  
  MAEAGGAGSPALPPAPPHGSPRTLATAAGSSASCGPATAVAAAGTAEGPGGGGSARIAVKKAQLRSAPRA
  KKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRGDLQQIIVSLTESCRSCSHALAAHVSHLENVSEEEM
  DRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSLEKKPPFEKPSIEQGVNNF
  VQYKFSHLPSKERQTTIELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKENYTRWLCYCNVPQFCDSL
  PRYETTKVFGRTLLRSVFTIMRRQLLEQARQEKDKLPLEKRTLILTHFPKFLSMLEEEVYSQNSPIWDQD
  FLSASSRTSPLGIQTVISPPVTGTALFSSNSTSHEQINGGRTSPGCRGSSGLEANPGEKRKMNNSHAPEE
  AKRSRVMGDIPVELINEVMSTITDPAGMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNK
  KILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSN
  EQVKGYGTHLMNHLKEYHIKHEILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCE
  LNPQIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRETGWKPSGKEKS
  KEPKDPEQLYSTLKNILQQVKNHPNAWPFMEPVKRTEAPGYYEVIRFPMDLKTMSERLRNRYYVSKKLFM
  ADLQRVFTNCKEYNPPESEYYKCASILEKFFFSKIKEAGLIDK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020005
• ORF Size: 2439 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020005.4
• RefSeq Size: 4309 bp
• RefSeq ORF: 2442 bp
• Locus ID: 18519
• UniProt ID: Q9JHD1
• Cytogenetics: 17 27.86 cM
• Gene Summary: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Also acetylates spermidine (By similarity).[UniProtKB/Swiss-Prot Function]