Tmem63a (NM_144794) Mouse Tagged ORF Clone

CAT#: MG210748

Tmem63a (tGFP-tagged) - Mouse transmembrane protein 63a (Tmem63a)

Product Images

Circular map for MG210748

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmem63a
• Synonyms: BC014795; Tmem64a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210748 representing NM_144794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCTCCCCGTTCCTGGATCCCTGGCCGTCCAAGGCTGTGTTTGTCCGTGAGCGCCTGGGTCTTG
  GCGAACGTCCCAATGACTCCTATTGCTACAACTCAGCCAAAAACAGCACTGTGCTCCAAGGGGTCACCTT
  TGGAGGCATCCCCACTGTCCTGCTCTTAGATGTCAGTTGCTTTCTGTTTCTAATCCTGGTGTTTTCCATC
  ATAAGGAGAAGATTCTGGGATTATGGCCGCATAGCCCTGGTATCAGAAGCAGGCAGTGAGGCCAGGTTCC
  AGAGACTGTCCTCTTCCTCCTCAGGGCAACAAGACTTTGAAAACGAGCTGGGATGCTGTCCTTGGCTGAC
  GGCCATCTTCCGCCTGCATGACGACCAGATCCTGGAGTGGTGCGGAGAGGACGCCATCCACTACCTGTCT
  TTTCAGAGGCACATCATCTTCCTGCTGGTGGTGATCAGCTTCTTGTCCCTGTGTGTCATCTTGCCTGTCA
  ACCTCTCGGGAGACTTGCTGGGTAAAGACCCTTACAGCTTTGGGAGGACAACGATAGCTAACCTGCAGAC
  TGACAATGACCTCCTCTGGCTGCACACTGTCTTCAGCGTCATCTACCTCTTCCTCACGGTGGGCTTCATG
  TGGCATCACACACGGTCCATCAGATACAAAGAGGAGAGTCTGGTGAGACAAACCTTGTTCATCACAGGAC
  TCCCCAGAGAAGCCAGAAAGGAGACTGTGGAGAGCCACTTCCGAGATGCGTATCCCACGTGTGAGGTGGT
  GGATGTCCAGCTGTGCTACAGCGTGGCTAAGCTGATCTACCTGTGCAAAGAGAGAAAGAAGACCGAGAAG
  AGCCTTACCTATTACACAAACCTGCAGGCGAAGACAGGCCGACGGACCCTCATCAACCCCAAGCCTTGTG
  GCCAGTTCTGCTGCTGTGAAGTTCAGGGTTGTGAGCGGGAAGATGCTATTTCTTACTACACTCGAATGAA
  TGACAGCCTGCTGGAGAGGATCACAGCCGAAGAGAGCCGGGTCCAGGACCAGCCCCTGGGAATGGCCTTT
  GTCACCTTCCGTGAGAAGTCCATGGCTACCTACATACTGAAAGACTTCAATGCCTGCAAGTGCCAGGGCC
  TTCGGTGCAAAGGGGAGCCCCAGCCATCCTCCTATAGCAGAGAGCTCTGTGTCTCCAAGTGGACGGTCAC
  TTTTGCCTCCTATCCTGAGGACATCTGCTGGAAGAACCTCTCCATCCAGGGCGTTCGCTGGTGGCTCCAG
  TGGCTGGGTATCAACTTCTCTCTGTTTGTGGTGCTGTTTTTCCTGACCACGCCCTCCATCATTATGTCTA
  CCATGGACAAGTTCAATGTTACCAAACCCATCCACGCACTGAATAACCCCGTCATCAGCCAGTTCTTCCC
  CACACTCCTGCTGTGGTCCTTCTCTGCGCTGCTTCCGTCCATTGTCTACTACTCCACGCTGCTGGAGTCC
  CACTGGACAAGGTCAGGGGAAAACCGGATCATGGTGTCGAAGGTCTACATATTTTTGATCTTCATGGTGC
  TGATCCTGCCCTCCCTCGGTCTCACCAGCTTGGATTTCTTCTTCCGGTGGCTCTTTGACAAAACTTCCTC
  GGAAACCTCAATCAGGCTAGAGTGTGTCTTCCTGCCAGACCAGGGTGCCTTCTTTGTGAACTACGTCATC
  GCCTCAGCCTTCATCGGCAGTGGCATGGAGCTGTTGCGCCTGCCCGGCCTCATCCTCTATACCTTCCGCA
  TGATCATGGCCAAGACGGCCGCTGACCGCAGGAATGTTAAGCAGAACCAGGCTTTTGAATACGAGTTTGG
  AGCCATGTATGCGTGGATGCTGTGTGTCTTCACCGTCATCATGGCTTACAGCATCACCTGCCCCATTATC
  GTGCCCTTTGGCCTCATCTACATCCTGCTTAAGCACATGGTGGACCGGCATAACCTCTACTTCGCTTACC
  TCCCGGCTAAACTGGAGAAGAGGATCCACTTCGCCGCCGTGAACCAGGCCCTGGCCGCCCCCATCCTCTG
  CCTCTTCTGGCTCTTCTTTTTCTCCTTCCTCCGTCTGGGTCTGACGGCCCCGGCCACGCTCTTCACCTTC
  CTGGTTGTTCTGCTCACCATCCTGGCCTGCCTGCTGTACACCTGCTTTGGATGCTTCAAGCACCTCAGCC
  CATGGAACTACAAGACAGAAGAATCAGCTAGTGACAAAGGAAGCGAAGCCGAGGCCCACGTGCCCCCGCC
  ATTCACACCCTATGTGCCTCGGATTCTGAACGGTTTGGCCTCAGAGAGGACAGCGTTATCCCCACAGCAG
  CAGCAGACCTACGGTGCCATCCGTAACATCAGTGGGACTCTTCCTGGGCAGCCCGTGGCTCAAGATCCTA
  GTGGCACTGCGGCTTATGCCTACCAGGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210748 representing NM_144794
  Red=Cloning site Green=Tags(s)
  
  MTSSPFLDPWPSKAVFVRERLGLGERPNDSYCYNSAKNSTVLQGVTFGGIPTVLLLDVSCFLFLILVFSI
  IRRRFWDYGRIALVSEAGSEARFQRLSSSSSGQQDFENELGCCPWLTAIFRLHDDQILEWCGEDAIHYLS
  FQRHIIFLLVVISFLSLCVILPVNLSGDLLGKDPYSFGRTTIANLQTDNDLLWLHTVFSVIYLFLTVGFM
  WHHTRSIRYKEESLVRQTLFITGLPREARKETVESHFRDAYPTCEVVDVQLCYSVAKLIYLCKERKKTEK
  SLTYYTNLQAKTGRRTLINPKPCGQFCCCEVQGCEREDAISYYTRMNDSLLERITAEESRVQDQPLGMAF
  VTFREKSMATYILKDFNACKCQGLRCKGEPQPSSYSRELCVSKWTVTFASYPEDICWKNLSIQGVRWWLQ
  WLGINFSLFVVLFFLTTPSIIMSTMDKFNVTKPIHALNNPVISQFFPTLLLWSFSALLPSIVYYSTLLES
  HWTRSGENRIMVSKVYIFLIFMVLILPSLGLTSLDFFFRWLFDKTSSETSIRLECVFLPDQGAFFVNYVI
  ASAFIGSGMELLRLPGLILYTFRMIMAKTAADRRNVKQNQAFEYEFGAMYAWMLCVFTVIMAYSITCPII
  VPFGLIYILLKHMVDRHNLYFAYLPAKLEKRIHFAAVNQALAAPILCLFWLFFFSFLRLGLTAPATLFTF
  LVVLLTILACLLYTCFGCFKHLSPWNYKTEESASDKGSEAEAHVPPPFTPYVPRILNGLASERTALSPQQ
  QQTYGAIRNISGTLPGQPVAQDPSGTAAYAYQES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144794
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144794.2, NP_659043.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 2415 bp
• Locus ID: 208795
• UniProt ID: Q91YT8
• Cytogenetics: 1 H4
• Gene Summary: Acts as an osmosensitive calcium-permeable cation channel.[UniProtKB/Swiss-Prot Function]