Ap1g2 (NM_007455) Mouse Tagged ORF Clone

CAT#: MG210676

Ap1g2 (tGFP-tagged) - Mouse adaptor protein complex AP-1, gamma 2 subunit (Ap1g2)

Product Images

Circular map for MG210676

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap1g2
• Synonyms: Adtg; Adtg2; G2ad
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210676 representing NM_007455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTGCATTCGTTGAGACTTCAGGACCTAATCGAAGAGATTCGCGGGGCCAAGACGCAGGCCCAGG
  AACGGGAGGTGATCCAGAAGGAGTGCGCCCAAATTCGGGCCTCCTTCCGCGATGGGGATCCCCTGCAGAG
  GCATCGCCAGCTGGCCAAACTGCTCTACGTCCACATGTTGGGCTACCCCGCCCACTTTGGACAGATGGAG
  TGCCTGAAACTGATCGCCTCCCCCAGATTCACAGACAAGAGGGTGGGCTACCTTGGGGCCATGCTTCTAT
  TGGATGAGAGGCACGATTCCCATCTGCTCATCACCAACAGCATCAAGAATGACCTGAGCCAAGGGAACCA
  GCCAGTTCAAGGCCTGGCCCTGTGTACTCTGAGTACCATGGGCTCTGCTGAGATGTGCCGGGACCTAGCC
  CCTGAGGTGGAAAAGCTGCTTCTGCAGCCCAGTCCCTATGTGCGGAAGAAGGCTATTTTGACTGCAGTGC
  ACATGATCCGGAAGGACCCTGAGCTCTCCGGCATCTTCCTCCCACCTTGTACCAAACTGCTTCGTGAGCG
  TCATCATGGCATCCAGCTGGGCACAGTCACGCTGATCACGGAGCTCTGTGAAAGAAACCCTGCAGCCCTC
  AGGCACTTTCGCAAGGTAGTGCCGCAGCTGGTACAGATCCTCCGGACTCTGGTGACTACAGGATACTCCA
  CGGAGCACAGCATCTCTGGAGTCAGCGACCCCTTCTTGCAGGTCCAGATACTCCGCCTACTTCGGATCCT
  GGGACGGAACCATGAAGAAAGTAGCGAGACCATGAACGACTTGCTGGCCCAGGTCGCCACCAACACAGAC
  ACCAGCCGAAATGCAGGCAACGCTGTCCTGTTGGAAACAGTGCTTACCATCATGGCCATCCACTCTGCTG
  CTGGCCTCCGGGTTCTAGCTGTTAACATTCTTGGTCGTTTCTTGCTCAACAATGACAAGAATATTAGGTA
  TGTGGCTCTGACATCATTGCTGCAGCTGGTGCAGTCTGACCACAGTGCTGTACAACGTCACCGGTCCACT
  GTGGTGGAGTGTCTACAGGAAACGGACGCCTCCCTTAGCAGGCGGGCCCTGGAGCTGAGCCTGGCTCTGG
  TGAACAGCTCCAATGTACGAGCCATGATGCAGGAGCTGCAGGCCTTTCTGGAGTCCTGCCCCCCTGATCT
  TCGGGCTGATTGTGCCTCAGGCATTCTGTTGGCTGCGGAGAGGTTTGCTCCCAGCAAGCGATGGCACATA
  GACACCATCCTGCACGTGCTGACCACGGCAGGAGCCCATGTGAGGGATGACGCAGTGGCCAACCTGACCC
  AGCTGATTGGAGAGGCCGAGGAGCTGCATACCTACTCTGTGCGCCGTCTCTACAGTGCCTTAGCAGAGGA
  TATCTCCCAGCAACCACTGGTTCAGGTGGCAGCCTGGTGCATTGGCGAGTATGGGGACCTCCTGCTGGAA
  GGTAACTGTGAGGAGACGGAGCCTTTTCAGGTGGAAGAAGAGGACGTGCTAGCACTGCTGGAAAAGGTGC
  TGCAGTCCCATATGTCCCTGCCAGCCACTCGGGGCTACGCCATCACAGCCCTCATGAAGCTGAGCACCCG
  ACTCCGGGGAGACAACAATCGTATTCGCCAGGTGGTGTCCATCTACGGGAGCTGTGTGGACTTAGAGCTG
  CAACAGCGGGCTGTGGAGTATAACACACTCTTCCAGAAGTACGACCACATGAGAGCCGCCATCCTAGAAA
  AGATGCCTCTTGTAGAGCGTGGTGACCCCCACGTTAAAGAGGGAGGGAAGGAGAAGCAAACGGAAGCCCA
  GCCCTTGGAAGTGACAGCCCCTGCCCCCACAGAACCCCAGGCCACCAAACTCTTAGATCTACTGGATCTC
  CTGGGTGACACTTCAGAGCCTCTCTCTTCTGGGCATGCCCAGCATCTTCCTCCTCAGACTCCTTCCCCAG
  GGGAAGCCTTAATTCATCTCCTTGACCTTCCCTGTACACCGCCACCCCCAGCTCCCATCCCCAGTGTCAG
  AGTGTTTGAGCGTGAGGGCCTACAGCTGGATCTTTCTTTCATGCGGCCCTTGGAGACCCCTGCTTTGCTC
  TTAGTCACTGCCACCACCACCAACTCCTCAAAGGAGGATGTTACCCACTTCGTTTGCCAGGCAGCTGTGC
  CCAAGAGTTTCCAGCTGCAGTTACAGGCCCCCAGTGGGAACACAATTCCAGCTCAGGGTGGTCTTCCCAT
  CACCCAGGTCTTCAGAATCCTCAATCCTAACCAGGCACCTTTGCGACTTAAGCTGCGCCTCACCTACAAC
  CACTCTGGCCAGCCAGTACAGGAGATCTTTGAGGTGGATAACTTGCCTGTGGAGACGTGGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210676 representing NM_007455
  Red=Cloning site Green=Tags(s)
  
  MVVHSLRLQDLIEEIRGAKTQAQEREVIQKECAQIRASFRDGDPLQRHRQLAKLLYVHMLGYPAHFGQME
  CLKLIASPRFTDKRVGYLGAMLLLDERHDSHLLITNSIKNDLSQGNQPVQGLALCTLSTMGSAEMCRDLA
  PEVEKLLLQPSPYVRKKAILTAVHMIRKDPELSGIFLPPCTKLLRERHHGIQLGTVTLITELCERNPAAL
  RHFRKVVPQLVQILRTLVTTGYSTEHSISGVSDPFLQVQILRLLRILGRNHEESSETMNDLLAQVATNTD
  TSRNAGNAVLLETVLTIMAIHSAAGLRVLAVNILGRFLLNNDKNIRYVALTSLLQLVQSDHSAVQRHRST
  VVECLQETDASLSRRALELSLALVNSSNVRAMMQELQAFLESCPPDLRADCASGILLAAERFAPSKRWHI
  DTILHVLTTAGAHVRDDAVANLTQLIGEAEELHTYSVRRLYSALAEDISQQPLVQVAAWCIGEYGDLLLE
  GNCEETEPFQVEEEDVLALLEKVLQSHMSLPATRGYAITALMKLSTRLRGDNNRIRQVVSIYGSCVDLEL
  QQRAVEYNTLFQKYDHMRAAILEKMPLVERGDPHVKEGGKEKQTEAQPLEVTAPAPTEPQATKLLDLLDL
  LGDTSEPLSSGHAQHLPPQTPSPGEALIHLLDLPCTPPPPAPIPSVRVFEREGLQLDLSFMRPLETPALL
  LVTATTTNSSKEDVTHFVCQAAVPKSFQLQLQAPSGNTIPAQGGLPITQVFRILNPNQAPLRLKLRLTYN
  HSGQPVQEIFEVDNLPVETWQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007455
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007455.5, NP_031481.2
• RefSeq Size: 2694 bp
• RefSeq ORF: 2376 bp
• Locus ID: 11766
• UniProt ID: O88512
• Cytogenetics: 14 C3
• Gene Summary: This gene encodes the gamma-2 subunit of the adaptor protein complex 1 (AP-1). AP-1 complex is a heterotetramer comprised of two heavy and one each of medium and small subunits. The encoded protein is a heavy subunit of AP-1 complex that regulates polarized sorting of cargo at the trans-Golgi network and endosomes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]