Spata20 (NM_144827) Mouse Tagged ORF Clone

CAT#: MG210668

Spata20 (tGFP-tagged) - Mouse spermatogenesis associated 20 (Spata20)

Product Images

Circular map for MG210668

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spata20
• Synonyms: Tisp78
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210668 representing NM_144827
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCACCATTCCTCACCACCCCCAAAACATAAGGGGGAGCACAAAGGCCACGGTCTCCCCCGTGGTT
  CAGAAAGGGGCAGCTCTTCCCGGGACAAGGACCGAAGTGCTACTACAGTTAGTAGTTCAGCGCCCATGCC
  TGCTGGAGGAAAGTCAAGCCGGACTAACTGTCCTCCACCTGCACCGCCGAAGACCGTCAATCGCCTGATC
  AATGAGAAGTCTCCATACCTCTTACAGCATGCCTACAACCCGGTGGATTGGTACCCCTGGGGACAGGAAG
  CCTTCGACAAGGCCAAAAAAGAAAACAAGCCGATTTTTCTCTCAGTGGGGTACTCTACCTGTCACTGGTG
  CCACATGATGGAGGAGGAATCCTTCCAGAATGAAGAGATAGGGCGCCTGCTCAATGAGAACTTCATCTGT
  GTCATGGTGGACCGAGAGGAGCGGCCAGATGTGGACAAAGTATACATGACTTTTGTGCAGGCCACCAGTA
  GTGGAGGAGGCTGGCCAATGAATGTGTGGCTGACCCCCGGCCTCCAGCCATTCGTTGGGGGCACCTACTT
  CCCGCCAGAGGATGGTTTGACTCGAGTTGGCTTCCGAACAGTGTTGATGAGAATCTGTGATCAGTGGAAA
  CTGAACAAGAACACCCTTCTGGAGAACAGCCAGCGCGTCACCACAGCCCTTCTGGCCCGATCTGAGATCA
  GCGTGGGTGACAGACAGATTCCAGCCTCTGCTGCCACCATGAACAGCCGTTGCTTCCAGCAGCTGGATGA
  GGGCTATGATGAGGAATACGGCGGCTTCGCCGAGGCCCCCAAGTTTCCCACACCTGTGATCCTGAACTTC
  CTGTTCTCCTATTGGCTCAGTCACCGGCTCACCCAGGATGGCTCCCGAGCTCAGCAGATGGCGTTGCACA
  CCCTGAAGATGATGGCCAACGGAGGCATCCAGGATCATGTGGGGCAGGGCTTTCACCGCTACTCCACGGA
  CCGACAGTGGCACATCCCTCACTTTGAGAAAATGCTGTATGACCAGGCCCAACTCTCGGTGGTCTATACC
  CAGGCCTTCCAGATCTCTGGCGATGAATTCTACGCTGATGTTGCTAAAGGCATCCTGCAGTATGTGACCC
  GGACTCTGAGCCATCGGTCTGGAGGCTTTTACAGTGCAGAGGATGCTGACTCCCCCCCGGAGAGAGGCAT
  GAAGCCCCAAGAGGGTGCTTACTATGTGTGGACAGTCAAAGAGGTCCAACAGCTACTCCCAGAGCCCGTG
  GTAGGCGCCAGCGAGCCGCTGACCTCAGGCCAGCTCCTAATGAAGCACTATGGACTCAGTGAAGTAGGCA
  ATATCAACTCCTCTCAGGATCCCAACGGGGAACTGCACGGCCAGAATGTCCTGATGGTGCGTTACTCACT
  AGAGCTGACTGCAGCCCGCTATGGCCTGGAGGTGGAGGCTGTGAGGGCCTTGCTCAACACAGGGCTGGAG
  AAGCTCTTCCAGGCCCGGAAACATCGGCCGAAAGCACACCTAGACAACAAGATGCTCGCTGCCTGGAACG
  GCTTGATGGTTTCTGGCTTTGCTGTGACGGGGGCTGCCCTGGGTATGGAAAAGTTAGTTGCCCAGGCCAC
  CAGCGGTGCCAAATTCCTTAAGCGGCACATGTTTGATGTGTCCAGCGGCCGCCTGAAGAGGACATGCTAT
  GCTGGCACCGGAGGAACTGTGGAGCAAAGCAACCCACCATGCTGGGGTTTCCTGGAGGACTATGCCTTTG
  TGGTACGGGGCCTCCTGGACCTGTACGAGGCCTCACAGGAGAGCTCATGGCTTGAGTGGGCTCTGCGCCT
  GCAGGACACACAGGACAAGCTCTTCTGGGACCCCCGAGGTGGTGGCTACTTCTGCAGTGAGGCTGAGCTG
  GGGGCAGACCTGCCTCTTCGTCTGAAGGATGACCAGGATGGCGCAGAGCCCAGTGCTAACTCTGTGTCGG
  CCCACAACCTCCTTCGGTTGCACAGCTTCACTGGCCACAAGGATTGGATGGACAAGTGTGTGTGCCTGCT
  GACAGCCTTCTCTGAGCGCATGCGCCGCGTCCCAGTGGCCTTGCCTGAGATGGTCCGTACCCTCTCAGCT
  CAACAGCAGACTCTGAAGCAGATTGTGATCTGTGGAGACCCCCAGGCCAAGGACACCAAGGCCCTGCTGC
  AATGTGTCCACTCCATCTACGTCCCTAACAAGGTGCTCATTCTGGCTGATGGAGACCCATCGAGCTTCCT
  GTCCCGTCAGCTTCCCTTCTTGAGCAGCCTTCGAAGAGTAGAAGACCGGGCCACAGTCTACATATTTGAG
  AACCAAGCCTGCTCCATGCCTATCACGGATCCCTGTGAGTTACGAAAGCTGCTACACCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210668 representing NM_144827
  Red=Cloning site Green=Tags(s)
  
  MSHHSSPPPKHKGEHKGHGLPRGSERGSSSRDKDRSATTVSSSAPMPAGGKSSRTNCPPPAPPKTVNRLI
  NEKSPYLLQHAYNPVDWYPWGQEAFDKAKKENKPIFLSVGYSTCHWCHMMEEESFQNEEIGRLLNENFIC
  VMVDREERPDVDKVYMTFVQATSSGGGWPMNVWLTPGLQPFVGGTYFPPEDGLTRVGFRTVLMRICDQWK
  LNKNTLLENSQRVTTALLARSEISVGDRQIPASAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPVILNF
  LFSYWLSHRLTQDGSRAQQMALHTLKMMANGGIQDHVGQGFHRYSTDRQWHIPHFEKMLYDQAQLSVVYT
  QAFQISGDEFYADVAKGILQYVTRTLSHRSGGFYSAEDADSPPERGMKPQEGAYYVWTVKEVQQLLPEPV
  VGASEPLTSGQLLMKHYGLSEVGNINSSQDPNGELHGQNVLMVRYSLELTAARYGLEVEAVRALLNTGLE
  KLFQARKHRPKAHLDNKMLAAWNGLMVSGFAVTGAALGMEKLVAQATSGAKFLKRHMFDVSSGRLKRTCY
  AGTGGTVEQSNPPCWGFLEDYAFVVRGLLDLYEASQESSWLEWALRLQDTQDKLFWDPRGGGYFCSEAEL
  GADLPLRLKDDQDGAEPSANSVSAHNLLRLHSFTGHKDWMDKCVCLLTAFSERMRRVPVALPEMVRTLSA
  QQQTLKQIVICGDPQAKDTKALLQCVHSIYVPNKVLILADGDPSSFLSRQLPFLSSLRRVEDRATVYIFE
  NQACSMPITDPCELRKLLHQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144827
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144827.4, NP_659076.2
• RefSeq Size: 2606 bp
• RefSeq ORF: 2373 bp
• Locus ID: 217116
• UniProt ID: Q80YT5
• Cytogenetics: 11 D
• Gene Summary: May play a role in fertility regulation.[UniProtKB/Swiss-Prot Function]