Pdxdc1 (NM_053181) Mouse Tagged ORF Clone

CAT#: MG210658

Pdxdc1 (tGFP-tagged) - Mouse RIKEN cDNA 2210010A19 gene (2210010A19Rik), transcript variant 1

Product Images

Circular map for MG210658

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdxdc1
• Synonyms: 2210010A19Rik; AA415817; Kiaa0251-hp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210658 representing NM_053181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGTCCCTGGAGAAGATAGCAGACCCTACATTAGCTGAAATGGGAAAAAACTTGAAAGAGGCGA
  TGCGGATGCTGGAGAAGAGCCCGAGAAGAACAGAGGAGGAAAATGGAAAGAAGCCAGTATCGGAGGATAT
  TCCAGGGCCACTCCAAGGCAGTGGACAAGATATGGTGAGCATCCTCCAGTTAGTTCAAAATCTGATGCAT
  GGAGATGAAGACGAGGAGCCCCAGAGCACCCGAATCCAAAACATTGGAGAGCAAGGTCACATGGCTTTGT
  TGGGTCATAGCCTCGGTGCCTATATTTCAACTCTGGATAAAGAGAAGCTGAGAAAGCTCACAACCAGGAT
  ACTTTCAGACACTACCTTATGGCTACGCCGGATTTTCAGGTACGAAAATGGATGTGCATATTTCCACGAG
  GAAGAGAGGGAAGGACTTGCTAAGATCTGTAGGCTTGCCATTCATTCTCGCTATGAAGACTTTGTTGTAG
  ATGGATTCAACGTGTTATATAACAAAAAGCCTGTCATATATCTTAGTGCTGCCGCTAGACCTGGCCTGGG
  CCAATACCTTTGTAATCAGCTTGGCTTACCCTTCCCCTGCCTGTGCCGTGTGCCCTGTAACACTATGTTT
  GGATCCCAGCATCAGATGGACGTTGCCTTTCTCGAGAAACTGATCAAAGATGACATAGAAAGAGGAAGAC
  TGCCCTTGCTGCTTGTTGCAAATGCTGGAACTGCAGCAGTTGGACACACAGACAAGATTGGACGTTTGAA
  GGAACTCTGTGAGCAGTACGGCATTTGGCTTCATGTGGAAGGTGTGAATCTGGCAACATTGGCTCTAGGT
  TATGTCTCCTCATCTGTGCTGGCTGCAACCAAGTGCGATAGCATGACACTAACTCCTGGCCTATGGCTAG
  GTTTGCCAGCTGTTCCTGCAGTGACACTCTACAAACATGACGATCCTGCCTTGACACTAGTTGCTGGTCT
  TACATCAAATAAGCCTGCAGACAAACTCCGAGCCCTACCACTCTGGTTGTCTCTACAGTACTTGGGACTT
  GATGGCATTGTGGAGAAAATAAAGCATGCTTGTCATCTGAGTCAACGGTTGCAAGAAAGTTTGAAGAAAG
  TGGACCACATCAAAATCTTGGTGGAAGATGAGCTCAGTTCTCCAGTAGTGGTGTTCAGGTTTTTCCAGGA
  ATTACCAGCCTCAGATTCAGCGTTTAAAGCTGTTCCTGTATCCAACATAGCACCTGCAGCAGTTGGCCGG
  GAAAGACACTCCTGTGATGCACTGAACCGCTGGTTGGGTGAACAGTTGAAACAGTTGGTGCCTCAGTGTG
  GCCTCACTGTCATAGACCTGGAGGTAGATGGCACATGTGTTCGGTTCAGCCCTCTGATGACAGCAGAAGG
  GTTAGGAACTCGAGGAGAGGATGTCGATCAGCTCATTACCTGCATCCAAAGCAAACTACCAGTCCTGACC
  TGCACACTACAGCTGCGGGAAGAATTCAAGCAGGAGGTAGAGGGGACAGCAGGGCTCCTGTATGTTGATG
  ACCCCAACTGGCCTGGAATCGGGGTTGTCAGGTATGAGCATGCGAATGATGATGACACCAGTTTGAAATC
  TGATCCAGAGGGGGAAAAAATACACACTGGACTTCTGAAAAAGTTAAATGAACTGGAATCTGACCTCACA
  TTTAAAATAGGCCCTGAGTACAAAAGCATGAAGAGCTGCATTTACATTGGCATGGCAAGTGATGATGTGG
  ACGTTTCTGAGCTAGTGGAGACCATTGCAGTCACAGCCCGGGAAATTGAGGAGAACTCAAGGCTTCTAGA
  GAACATGACAGAAGTTGTTCGGAAGGGAATTCAGGAGGCACAGGTTCAGCTACAGAAGGCAAATGAGGAG
  CGGCTTCTGGAAGAGGGAGTGTTGCGGCAAATCCCTGTAGTAGGATCCGTGCTGAATTGGTTTTCTCCAG
  TCCAGGCTTCACAGAAGGGAAGAAGTTTTAACTTAACAGCAGGCTCTCTGGAGTCCACAGAATACACCTA
  TGTCCACAAAGTACAAGGTACTGGGGTGACACCACCTCCCACACCCCTAGGCACTCGAAGCAAACAGAGG
  CTTCCAGGTCAGAAGCCTTTTAAAAGGTCCCTGAGAGGCTCGGATGCTGTGAGTGAGACCAGCTCAGTCA
  GCCACATTGAAGACCTGGAAAAGGTGGAACAGCTGTCCAGTGGGCTAGAGCATGACAACCTAGAGGCCCA
  CAGCCCTGAGCAGCCTCCCAGGGCTGCTGATCTCACGGCCAGACAGACTGAGGCCCTGCAGAACCAGGCC
  CAGCATCAGGAAGATGACCATTCACAGGTAGAGGAACTGGAGCGCTTAAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210658 representing NM_053181
  Red=Cloning site Green=Tags(s)
  
  MDASLEKIADPTLAEMGKNLKEAMRMLEKSPRRTEEENGKKPVSEDIPGPLQGSGQDMVSILQLVQNLMH
  GDEDEEPQSTRIQNIGEQGHMALLGHSLGAYISTLDKEKLRKLTTRILSDTTLWLRRIFRYENGCAYFHE
  EEREGLAKICRLAIHSRYEDFVVDGFNVLYNKKPVIYLSAAARPGLGQYLCNQLGLPFPCLCRVPCNTMF
  GSQHQMDVAFLEKLIKDDIERGRLPLLLVANAGTAAVGHTDKIGRLKELCEQYGIWLHVEGVNLATLALG
  YVSSSVLAATKCDSMTLTPGLWLGLPAVPAVTLYKHDDPALTLVAGLTSNKPADKLRALPLWLSLQYLGL
  DGIVEKIKHACHLSQRLQESLKKVDHIKILVEDELSSPVVVFRFFQELPASDSAFKAVPVSNIAPAAVGR
  ERHSCDALNRWLGEQLKQLVPQCGLTVIDLEVDGTCVRFSPLMTAEGLGTRGEDVDQLITCIQSKLPVLT
  CTLQLREEFKQEVEGTAGLLYVDDPNWPGIGVVRYEHANDDDTSLKSDPEGEKIHTGLLKKLNELESDLT
  FKIGPEYKSMKSCIYIGMASDDVDVSELVETIAVTAREIEENSRLLENMTEVVRKGIQEAQVQLQKANEE
  RLLEEGVLRQIPVVGSVLNWFSPVQASQKGRSFNLTAGSLESTEYTYVHKVQGTGVTPPPTPLGTRSKQR
  LPGQKPFKRSLRGSDAVSETSSVSHIEDLEKVEQLSSGLEHDNLEAHSPEQPPRAADLTARQTEALQNQA
  QHQEDDHSQVEELERLR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053181
• ORF Size: 2361 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053181.1
• RefSeq Size: 3223 bp
• RefSeq ORF: 2364 bp
• Locus ID: 94184
• UniProt ID: Q99K01
• Cytogenetics: 16 A1