Cep85 (NM_144527) Mouse Tagged ORF Clone

CAT#: MG210523

Cep85 (tGFP-tagged) - Mouse coiled-coil domain containing 21 (Ccdc21)

Product Images

Circular map for MG210523

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cep85
• Synonyms: 2410030J07Rik; AI173272; AI173504; Ccdc21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210523 representing NM_144527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGCAAGAAAAATACCCAAATGACAGGTCTCATGCTACTTCACCAGGTTCCAATGTGATTCAAA
  AAGGCAGTTCTCTGGGAACTGAGTGGCAGACACCAGTTATCTCAGAGACCTTTCGGAGCCGCTTCAGCCG
  CTGCTCAAGCATTGCTGACAGTGGGGACACAGCCATTGGGACATCATGCTCAGACATTGCAGAGGATTTT
  TGCAGCTCAAGTGGCAGCCCTTCCTTCCAGCCAATCAAAAGCCACATAACCATTCCAACAGCCCATGTGA
  TGCCTTCTACTTTGGGGGCCTCTCCTGCCAAACCAAATTCTGCACCTTCCGGGCCTTCTTCCGCTAAGCT
  GCCCTTGTCAGGATTGACTGAAGGCGTGGGGATGACAAGAAATGGAGACTTCGGAGCTGTGAAGCGCTCT
  CCAGGCCTAGCAAGGGATTTTATGTACCTCCCTAGTGCTGCCGGGGAGAATGGGAGTCAGCAGTCCTGGT
  TCCCAGCAGTGGGCCACGAGAGAGAGGGGGAGATGAGGAAGTTCGATGTTCCTAGCATGGAGTCTACCCT
  TAACCAGCCAGCCATGCTAGAGACGTTATACTCAGATCCACATTACCGAGCCCATTTCCCCAACCCAAGA
  CCTGATACAAATAAGGATGTATACAAAGTATTGCCAGAATCCAAGAAGGCACCGGGCAGTGGTGCAGTAT
  TTGAGAGGAACGGACCACATGCTAGCAGTAGTGGGGTGCTCCCTTTGGGACTCCAGCCTGCGCCTGGACT
  TTCCAAGTCACTATCCTCTCAGGTGTGGCAACCAAGTCCTGACCCTTGGCATCCTGGAGAACAATCCTGT
  GAACTCAGTACTTGTCGACAGCAGTTGGAATTGATCCGTTTACAGATGGAGCAAATGCAGCTTCAGAACG
  GAGCCATGTGTCACCATCCTGCTGCTTTCGCTCCATTACTGCCCACCCTAGAGCCAGCACAGTGGCTCAG
  CATCCTGAACAGTAACGAGCATCTCCTGAAGGAGAAGGAGCTCCTCATTGACAAGCAAAGGAAGCATATC
  TCTCAGCTGGAGCAGAAAGTGCGAGAGAGTGAACTGCAAGTCCACAGTGCCCTTTTGGGCCGCCCTGCCC
  CCTTTGGGGATGTCTGCTTATTGAGGCTACAGGAGTTGCAGCGAGAGAACACTTTCTTACGGGCACAGTT
  TGCACAGAAGACAGAAGCCCTGAGCAAGGAGAAGATGGAGCTTGAAAAGAAACTCTCTGCATCTGAAGTT
  GAAATTCAGCTCATTAGGGAGTCTCTAAAAGTGACACTACAGAAGCATTCGGAGGAGGGGAAGAAACAGG
  AGGAAAGGGTCAAAGGTCGTGATAAACATATCAATAATTTGAAAAAGAAATGTCAGAAGGAATCAGAGCA
  GAACCGGGAGAAGCAGCAGCGTATTGAAACCTTGGAGCGCTATCTAGCTGACCTGCCCACCCTAGAAGAC
  CATCAGAAACAGACGGAGCAGCTTAAGGACGCTGAATTAAAGAACACAGAACTGCAAGAGAGAGTGGCTG
  AGCTGGAGACTTTGCTGGAGGACACCCAGGCAACCTGCAGAGAGAAGGAGGTTCAGCTGGAAAGTCTGAG
  ACAAAGAGAAGCAGACCTCTCCTCTGCTAGACATAGCTTTCAAGATAAACAGTCTGTGGAGGAAGCCAAT
  GGAGAAAACCTCAGAGTGGACATGGAATCCCAGCAGAAGGAATGTGACTCCCTCCGAAAGATGGTGGAGA
  GGCAACAGCTGAAGATGGAACAGCTGCACTCCCAAGTCCAGAGCCAAAAGCAAGAACTGGCACAAGAAGA
  GGGAATCAACCAGGCACTGAGAGAGGAGGCCCAGCGGAGGGAGACGGCCCTGCAGCAGATGCGCACAGCC
  GTGAAGGAGCTTTCAGTGCAAAACCAGGACCTGATTGAGAAGAATCTGACGCTGCAAGAGCACTTGCGCC
  AGGCCCAGCCAGGGTCCTCATCTTCACCAGACTCAGCCCAGCTGGCCTGTGAGCTGCACCAGGAACTGGC
  CAGTTGCCTTCAAGATCTGCAAGCTGTCTGCAGCATTGTGACCCAGAGGGCCCAGGGCCACAATCCTAAC
  CTCTCACTACTCCTAGGCATTCACTCCACCCAGCACCCAGGGACGCAGCTAGATCTACAAAAGCCAGATG
  TGATCAGGAGGAAACTAGAAGAGGTTCAACAGCTGCGCCATGACATTGAGGACTTAAGGACCAGCCTGTC
  AGACAGATATGCCCAGGACATGGGAGAAAACTGTGCCACACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210523 representing NM_144527
  Red=Cloning site Green=Tags(s)
  
  MAMQEKYPNDRSHATSPGSNVIQKGSSLGTEWQTPVISETFRSRFSRCSSIADSGDTAIGTSCSDIAEDF
  CSSSGSPSFQPIKSHITIPTAHVMPSTLGASPAKPNSAPSGPSSAKLPLSGLTEGVGMTRNGDFGAVKRS
  PGLARDFMYLPSAAGENGSQQSWFPAVGHEREGEMRKFDVPSMESTLNQPAMLETLYSDPHYRAHFPNPR
  PDTNKDVYKVLPESKKAPGSGAVFERNGPHASSSGVLPLGLQPAPGLSKSLSSQVWQPSPDPWHPGEQSC
  ELSTCRQQLELIRLQMEQMQLQNGAMCHHPAAFAPLLPTLEPAQWLSILNSNEHLLKEKELLIDKQRKHI
  SQLEQKVRESELQVHSALLGRPAPFGDVCLLRLQELQRENTFLRAQFAQKTEALSKEKMELEKKLSASEV
  EIQLIRESLKVTLQKHSEEGKKQEERVKGRDKHINNLKKKCQKESEQNREKQQRIETLERYLADLPTLED
  HQKQTEQLKDAELKNTELQERVAELETLLEDTQATCREKEVQLESLRQREADLSSARHSFQDKQSVEEAN
  GENLRVDMESQQKECDSLRKMVERQQLKMEQLHSQVQSQKQELAQEEGINQALREEAQRRETALQQMRTA
  VKELSVQNQDLIEKNLTLQEHLRQAQPGSSSSPDSAQLACELHQELASCLQDLQAVCSIVTQRAQGHNPN
  LSLLLGIHSTQHPGTQLDLQKPDVIRRKLEEVQQLRHDIEDLRTSLSDRYAQDMGENCATQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144527
• ORF Size: 2283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144527.3, NP_653110.3
• RefSeq Size: 3878 bp
• RefSeq ORF: 2286 bp
• Locus ID: 70012
• UniProt ID: Q8BMK0
• Cytogenetics: 4 D3
• Gene Summary: Acts as a negative regulator of NEK2 to maintain the centrosome integrity in interphase. Suppresses centrosome disjunction by inhibiting NEK2 kinase activity.[UniProtKB/Swiss-Prot Function]