Dnttip2 (NM_153806) Mouse Tagged ORF Clone

CAT#: MG210503

Dnttip2 (tGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 2 (Dnttip2)

Product Images

Circular map for MG210503

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnttip2
• Synonyms: 4930588M11Rik; AA408582; AU014960; HSU15552
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210503 representing NM_153806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTCACCAGGTCCGGGCTCTCCCGGACCAGGCTCCAAGAAAGCTCCCAGCAGAAGAGGTCTGCTC
  CTCGGAGAATTGGGACACATCTAGAAAGCACAAAGGAATCGGGATCCGATGGCAGCACAGCTGAATCGCA
  GCCTGCTGAGAAACAGCACTCACGCAGCAGCAGCAGAACCACAGGCCCAGCGGAGATCATTGTGCTTATT
  TCTGATGACGAGGCTTCTGAAACGGAGTCCCACACTTCTGGGGTTACTTCTGTGTTAGAGGACCAAGAGC
  CCATTGTAAGAGTAACTAGGAAACGGCAGATCGTAATTGCTTCCACCTCAAAGTCCACTGTTAGGAAAAG
  GCAGAAAGTAGCCCCTCAGCATGCATCTGCTGATGAAGTTGTTGTTTCTGAAGCAGAGTCTCATGTTTCA
  GGGGTTTCTATGGTTGTACCTTCCACAGAGAGAAGCAGCAGAAATAAGGCTAATTCTCAAAGAGATTCAA
  GCCAAGAATCACAGTCGGGAACTGTATCTGATGCTGAGTTGTCCTGCTCAGGCATTTCATCGTTAGAGAT
  CTTGCCGAGAACAACAGCCAGGAATGTGAAGAAGAAATTACAGTTCCCAGCTGAGAAAAACGATACTAAG
  ATTACACCAGGAAATAAAAAGCAAATTGTGGGTATGTCTGTGTGTTCAGAGGACTCAGATGCTACTCAGT
  TATCAGCAAGACCACTTTCTCAGAGAAATATGCCAAATGTTTCTGATAGTGAAACTTACAACAGTGACTT
  TGATGATTCCAGCCCCAGAAATTCAGGGAAAAAACTGACAGCCCAAAACCACCAAAATTTGCATATCCAA
  GAGGAAAAACGAGCCAATGTTGTGTCTCTTACAGAAGTTAGAAAGGAGAATTGTAAGAGTCTGGATGAAG
  AGGACCTCAAAATCACAGAGGAAAAAGTAATTAATGAGAAAGATTCCCAGCGGAGTCTTTCTGAAGCTCA
  AGACACCAGCCTTCAGCAGTCAGTGTCTCAGAATCATTCAAGCACCCCAAATAAAAAACCCACATTCCAG
  CTTTCAAGTCCAGATCGGAAGGCTCTAATGAAATCATTAGAGCACAAGTTTGCAGTTGTGAATGTAGAAA
  GGTGGAATGACAAGAGAGGGGGCAGCGGAAAAAAGAGTGACTTGGCACAGCTCGGTGGTGGTGGTGGTGG
  TGGTGATGACAACGAGCCCACGGGTGCAGGTATCAGTGATGACAAGAGCAGCCAAAGTGGTGTCCCTCTT
  GAATGTGATACCAAACCATGCAAGTCTGAACTCAGCATGACTCAGGATACTACGGACAGTCCTGTCTTAT
  TATTTCTTAGCAGCGATGAAAGCCAGCAGTCTGACAGCAGTGAGAATGAAAGGGACACTTTGTGTTCTGT
  TGAAAACAATGGCCAAAAGGAGGCATCAGCTGAGGACCTGGAAGATGCCGCCTGTGACAGTGCACTGTTT
  GTAATTGACAAAACTCCTGGATTGAGTGCTGACAAAAACTTTTACCTAGAAGACAAGGCTCCGAGTGAAG
  TTGCTATTGAGGAAGAAAAAGAGGAAGAGGAGAAAGAGGAGGAAAATAGTGAAGAAGACTCATCAGACAG
  TGATGAGAATAAGGATGAGTCTAGTGACGAAGAAGACTTGCTCAGTAACACCAAGTCTAAGCTTCTGAAA
  TTGACAAGTAGCAGCATAGACCCTGGACTGAACATCAAGCAGTTGGGTGGTCTGTATATTAATTTCAATG
  TAGACAAACTGCAGCCTCACAAGGAAACTCTAACCCAGATCAAGGAGAAAAAGAAAAATGAGCTTCTGCA
  GAAAGCTGTCATTACACCTGATTTTGAAAAAAAGCATTGTGTCCCACCATACAGTGAGTCCAAGCATCGA
  CTTCAGAAGCAGCGCAGGAAAGAGCGACAAAAAACAGCCGGTAATGGTTGGTTTGGCATGAAAGCCCCAG
  AACTGACAGATGAACTGAAAAATGACCTCAGAGCATTGAAGATGAGAGCAGGCATGGACCCCAAAAGGTT
  TTACAAGAAAAATGACAGAGATGGTTTCCCTAAGTACTTCCAGGTTGGAACCATTGTTGATAATCCAGCC
  GACTTCTACCATTCACGAATTCCCAAGAAACAGAGGAAAAAGACGATTGTGGAAGAACTGCTGGCTGACT
  CTGAGTTCAGGAGATTTAATCGAAGGAAGTATTCAGAAATCATGGCTGAAAAAGCAGCAAATGCAGAAGG
  AAAGAAGTTCAAGAAAAAGAAGAAATTTCGCAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210503 representing NM_153806
  Red=Cloning site Green=Tags(s)
  
  MVVTRSGLSRTRLQESSQQKRSAPRRIGTHLESTKESGSDGSTAESQPAEKQHSRSSSRTTGPAEIIVLI
  SDDEASETESHTSGVTSVLEDQEPIVRVTRKRQIVIASTSKSTVRKRQKVAPQHASADEVVVSEAESHVS
  GVSMVVPSTERSSRNKANSQRDSSQESQSGTVSDAELSCSGISSLEILPRTTARNVKKKLQFPAEKNDTK
  ITPGNKKQIVGMSVCSEDSDATQLSARPLSQRNMPNVSDSETYNSDFDDSSPRNSGKKLTAQNHQNLHIQ
  EEKRANVVSLTEVRKENCKSLDEEDLKITEEKVINEKDSQRSLSEAQDTSLQQSVSQNHSSTPNKKPTFQ
  LSSPDRKALMKSLEHKFAVVNVERWNDKRGGSGKKSDLAQLGGGGGGGDDNEPTGAGISDDKSSQSGVPL
  ECDTKPCKSELSMTQDTTDSPVLLFLSSDESQQSDSSENERDTLCSVENNGQKEASAEDLEDAACDSALF
  VIDKTPGLSADKNFYLEDKAPSEVAIEEEKEEEEKEEENSEEDSSDSDENKDESSDEEDLLSNTKSKLLK
  LTSSSIDPGLNIKQLGGLYINFNVDKLQPHKETLTQIKEKKKNELLQKAVITPDFEKKHCVPPYSESKHR
  LQKQRRKERQKTAGNGWFGMKAPELTDELKNDLRALKMRAGMDPKRFYKKNDRDGFPKYFQVGTIVDNPA
  DFYHSRIPKKQRKKTIVEELLADSEFRRFNRRKYSEIMAEKAANAEGKKFKKKKKFRN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153806
• ORF Size: 2274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153806.1, NP_722501.1
• RefSeq Size: 2427 bp
• RefSeq ORF: 2277 bp
• Locus ID: 99480
• UniProt ID: Q8R2M2
• Cytogenetics: 3 G1
• Gene Summary: Regulates the transcriptional activity of DNTT and ESR1. May function as a chromatin remodeling protein (By similarity).[UniProtKB/Swiss-Prot Function]