Foxm1 (NM_008021) Mouse Tagged ORF Clone

CAT#: MG210493

Foxm1 (tGFP-tagged) - Mouse forkhead box M1 (Foxm1)

Product Images

Circular map for MG210493

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Foxm1
• Synonyms: AA408308; AW554517; BB238854; Fkh16; Foxm1b; HFH-11B; MPHOSPH2; Mpm2; WIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210493 representing NM_008021
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACCAGCCCCCGCCGGCCACTGATTCTCAAAAGACGGAGGCTGCCCCTTCCTGTCCAGAATGCCC
  CGAGTGAAACATCAGAGGAAGAAGCAAAGAGGTCCCCTGCCCAGCCGGAGCCTGCTCCAGCACAGGCCTC
  CCAAGAGGTGGCAGAGTCCAGCTCTTGCAAATTTCCAGCCGGAATCAAGATTATCAACCACCCCACCACG
  CCCAACACACAAGTGGTGGCCATCCCCAGCAACGCTGATATCCAGAGCATCATCACAGCGCTAACTGCCA
  AAGGGAAGGAGAGCGGCAGCAGCGGGCCCAACCGGTTCATCCTCATCAGCTCTGGGGGGCCCTCCTCTCA
  CCCTTCCCAGCCTCAAGCCCACAGCAGCCGGGATTCCAAGAGAGCAGAGGTGATCACGGAGACGTTGGGA
  CCGAAGCCAGCAGCTAAGGGTGTGCCTGTTCCCAAGCCTCCTGGAGCCCCTCCAAGGCAAAGACAGGAGA
  GCTATGCTGGTGGTGAGGCGGCAGGCTGCACGCTGGACAACAGCTTAACCAATATCCAGTGGCTTGGAAA
  GATGAGTTCTGACGGGCTGGGCCCCTGCAGCGTTAAGCAGGAACTGGAAGAGAAGGAGAATTGTCACCTG
  GAGCAGAATCGGGTTAAGGTTGAGGAGCCCTCAGGAGTGTCAACATCTTGGCAGGACTCTGTGTCTGAGA
  GGCCACCCTACTCTTATATGGCCATGATACAGTTTGCCATCAACAGCACTGAGAGAAAGCGCATGACCTT
  GAAGGACATCTACACTTGGATTGAGGACCACTTCCCTTACTTTAAGCACATTGCCAAGCCAGGCTGGAAG
  AACTCTATTCGTCACAACCTTTCTCTCCATGACATGTTTGTTCGAGAGACATCTGCCAATGGCAAGGTCT
  CCTTCTGGACCATTCACCCAAGTGCCAATCGCTACTTGACATTGGACCAAGTGTTTAAGCCACTGGAACC
  AGGGTCTCCACAATCGCCCGAGCACTTGGAATCACAGCAGAAACGACCCAATCCTGAGCTCCATAGAAAT
  GTGACCATCAAAACTGAAATCCCACTGGGCGCACGGCGAAAGATGAAGCCTCTGCTCCCTCGGGTTAGCT
  CATACCTGGTGCCCATCCAGTTCCCAGTGAACCAGTCCCTGGTGTTACAGCCCTCAGTGAAGGTTCCCTT
  GCCTCTGGCAGCATCGCTTATGAGCTCAGAGCTCGCCCGTCATAGCAAGCGAGTCCGCATTGCACCCAAG
  GTGTTGCTATCCAGTGAAGGAATAGCCCCGCTTCCTGCCACAGAACCACCGAAGGAGGAGAAACCCCTGC
  TTGGAGGGGAAGGGCTGTTGCCTTTACTTCCTATTCAGTCCATTAAGGAAGAAGAAATGCAGCCTGAGGA
  GGACATAGCACACTTAGAGAGGCCTATCAAAGTGGAGAGCCCTCCCTTGGAAGAGTGGCCCTCTCCGTGT
  GCATCGCTCAAAGAGGAGCTATCCAACTCCTGGGAAGATTCTTCCTGCTCTCCTACCCCAAAGCCCAAGA
  AGTCCTACTGTGGGCTCAAGTCCCCAACGCGCTGTGTCTCAGAAATGCTGGTGACTAAGCGAAGAGAGAA
  AAGGGAGGTGAGCCGATCCCGGAGGAAACAGCACCTTCAGCCCCCCTGTCTGGATGAGCCTGACCTGTTC
  TTCTCAGAGGACTCCAGCACATTTCGGCCAGCCGTGGAGCTCCTGGCGGAGTCTTCCGAGCCTGCACCCC
  ACCTCAGCTGCCCTCAGGAAGAGGGAGGACCTTTCAAGACCCCCATCAAGGAGACATTGCCTGTCTCCTC
  CACTCCTAGCAAGTCTGTGCTCTCTAGAGACCCTGAGTCCTGGAGGCTCACACCTCCAGCCAAAGTTGGG
  GGGTTAGATTTCAGCCCAGTACGAACCCCCCAGGGTGCCTTTGGCCTTCTGCCTGACTCACTGGGGTTGA
  TGGAGCTGAATACCACCCCTTTGAAAAGTGGTCCTCTCTTTGACTCGCCCCGGGAGCTCCTCAACTCAGA
  GCCCTTTGACCTGGCCTCTGACCCCTTCGGCAGCCCTCCACCACCACATGTGGAAGGCCCCAAGCCTGGC
  TCCCCCGAGCTGCAGATTCCCAGCCTTTCAGCCAACCGTTCTCTCACAGAAGGCCTTGTCCTGGACACAA
  TGAATGATAGCCTCAGCAAGATCCTTCTAGACATCAGTTTCCCTGGCCTGGAGGAGGACCCTCTGGGCCC
  TGACAACATCAACTGGTCTCAGTTCATCCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210493 representing NM_008021
  Red=Cloning site Green=Tags(s)
  
  MRTSPRRPLILKRRRLPLPVQNAPSETSEEEAKRSPAQPEPAPAQASQEVAESSSCKFPAGIKIINHPTT
  PNTQVVAIPSNADIQSIITALTAKGKESGSSGPNRFILISSGGPSSHPSQPQAHSSRDSKRAEVITETLG
  PKPAAKGVPVPKPPGAPPRQRQESYAGGEAAGCTLDNSLTNIQWLGKMSSDGLGPCSVKQELEEKENCHL
  EQNRVKVEEPSGVSTSWQDSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWK
  NSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLEPGSPQSPEHLESQQKRPNPELHRN
  VTIKTEIPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPK
  VLLSSEGIAPLPATEPPKEEKPLLGGEGLLPLLPIQSIKEEEMQPEEDIAHLERPIKVESPPLEEWPSPC
  ASLKEELSNSWEDSSCSPTPKPKKSYCGLKSPTRCVSEMLVTKRREKREVSRSRRKQHLQPPCLDEPDLF
  FSEDSSTFRPAVELLAESSEPAPHLSCPQEEGGPFKTPIKETLPVSSTPSKSVLSRDPESWRLTPPAKVG
  GLDFSPVRTPQGAFGLLPDSLGLMELNTTPLKSGPLFDSPRELLNSEPFDLASDPFGSPPPPHVEGPKPG
  SPELQIPSLSANRSLTEGLVLDTMNDSLSKILLDISFPGLEEDPLGPDNINWSQFIP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008021
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008021.4, NP_032047.4
• RefSeq Size: 4391 bp
• RefSeq ORF: 2274 bp
• Locus ID: 14235
• Cytogenetics: 6 62.98 cM