Zbtb20 (NM_019778) Mouse Tagged ORF Clone

CAT#: MG210403

Zbtb20 (tGFP-tagged) - Mouse zinc finger and BTB domain containing 20 (Zbtb20)

Product Images

Circular map for MG210403

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zbtb20
• Synonyms: 1300017A20Rik; 7330412A13Rik; A930017C21Rik; D16Wsu73e; DPZF; HOF; ODA-8S; Oda8; Zfp288
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210403 representing NM_019778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGAACGGAAGAAACCCAAGACAGCTGAAAACCAGAAGGCATCTGAGGAGAATGAGATTACTCAGC
  CGGGCGGATCCAGCGCCAAGCCGGCCCTTCCCTGCCTGAACTTTGAAGCTGTTTTGTCTCCAGCCCCAGC
  CCTCATCCACTCGACACATTCACTGACAAACTCTCACGCTCACACCGGGTCATCTGATTGTGACATCAGT
  TGCAAGGGGATGACCGAGCGCATTCACAGCATCAACCTTCACAACTTCAGCAATTCCGTGCTCGAGACCC
  TCAACGAGCAGCGCAACCGTGGCCACTTCTGTGACGTGACGGTTCGCATCCACGGGAGCATGCTGCGCGC
  ACATCGCTGCGTGCTGGCAGCCGGCAGCCCCTTCTTCCAAGACAAGCTGCTGCTGGGCTACAGCGACATC
  GAAATCCCGTCGGTGGTGTCCGTACAATCGGTGCAAAAGCTCATTGACTTCATGTACAGCGGTGTGCTGA
  GAGTCTCACAGTCGGAAGCTCTGCAGATCCTCACAGCCGCCAGCATCCTGCAGATCAAAACAGTCATAGA
  TGAGTGCACTCGCATCGTGTCACAGAACGTGGGCGATGTGTTCCCAGGCATCCAGGATTCTGGCCAGGAC
  ACACCAAGAGGCACACCAGAGTCAGGCACATCTGGCCAGAGCAGTGACACGGAATCAGGCTACCTGCAGA
  GCCACCCACAGCATAGTGTGGACCGAATCTACTCCGCACTCTACGCCTGCTCCATGCAGAATGGCAGCGG
  CGAGCGCTCCTTCTACAGTGGTGCAGTGGTCAGCCACCACGAAACAGCTCTCGGCCTGCCCCGTGACCAC
  CACATGGAAGACCCTAGCTGGATCACACGCATTCATGAGCGCTCCCAGCAAATGGAGCGCTACCTGTCCA
  CCACCCCTGAGACCACGCACTGCCGGAAGCAGCCCCGGCCTGTGCGTATCCAGACCCTGGTGGGTAACAT
  CCACATCAAGCAGGAAATGGAAGATGACTATGACTACTATGGGCAGCAAAGGGTGCAGATCCTAGAACGC
  AATGAATCCGAGGAGTGCACAGAAGACACTGACCAAGCAGAGGGCACTGAGAGCGAGCCCAAAGGTGAAA
  GCTTTGATTCTGGGGTCAGCTCCTCCATCGGCACCGAACCTGACTCAGTGGAGCAACAGTTTGGGGCAGC
  AGCCCCAAGGGACGGTCAGGCAGAACCCGCCCAACCTGAGCAGGCAGCAGAAGCCCCAGCTGAGAGCAGT
  GCCCAGCCAAACCAGCTAGAACCAGGTGCCTCCTCTCCTGAGAGAAGCAACGAGTCAGAGATGGACAACA
  CAGTCATCACTGTCAGTAACAGCTCCGATAAGGGCGTCCTACAGCAGCCTTCAGTCAACACATCCATCGG
  GCAGCCATTGCCAAGTACCCAGCTCTATTTACGCCAGACAGAAACCCTCACCAGCAACCTGAGGATGCCT
  CTGACCTTGACCAGCAACACACAGGTCATTGGCACCGCTGGCAACACCTATCTGCCAGCCCTCTTCACTA
  CCCAACCCGCGGGCAGTGGCCCCAAGCCTTTTCTCTTCAGCCTGCCGCAGCCCCTGACAGGCCAGCAGAC
  CCAGTTTGTGACAGTGTCCCAGCCCGGTCTGTCCACCTTTACTGCACAGCTGCCAGCGCCACAGCCCCTG
  GCCTCATCTGCAGGCCACAGCACAGCCAGTGGGCAAGGCGACAAAAAGCCTTATGAGTGCACTCTCTGCA
  ACAAGACTTTCACAGCCAAACAGAACTACGTCAAGCACATGTTCGTACATACAGGTGAGAAGCCCCACCA
  GTGCAGCATCTGCTGGCGCTCCTTCTCCTTGAAGGATTACCTTATCAAGCACATGGTGACGCACACCGGC
  GTGAGAGCGTACCAGTGTAGCATCTGCAACAAGCGCTTCACCCAGAAGAGTTCCCTCAACGTGCACATGC
  GCCTGCACCGCGGGGAGAAGTCCTATGAGTGCTACATCTGCAAAAAGAAGTTCTCCCACAAGACCCTGCT
  GGAGCGACACGTGGCCCTGCACAGTGCCAGCAACGGGACCCCTCCGGCAGGCACGCCCCCAGGTGCCCGC
  GCGGGTCCGCCAGGCGTGGTGGCCTGCACAGAGGGGACCACTTACGTCTGCTCCGTCTGCCCAGCAAAGT
  TTGACCAAATCGAGCAGTTCAACGACCACATGAGGATGCATGTGTCTGACGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210403 representing NM_019778
  Red=Cloning site Green=Tags(s)
  
  MLERKKPKTAENQKASEENEITQPGGSSAKPALPCLNFEAVLSPAPALIHSTHSLTNSHAHTGSSDCDIS
  CKGMTERIHSINLHNFSNSVLETLNEQRNRGHFCDVTVRIHGSMLRAHRCVLAAGSPFFQDKLLLGYSDI
  EIPSVVSVQSVQKLIDFMYSGVLRVSQSEALQILTAASILQIKTVIDECTRIVSQNVGDVFPGIQDSGQD
  TPRGTPESGTSGQSSDTESGYLQSHPQHSVDRIYSALYACSMQNGSGERSFYSGAVVSHHETALGLPRDH
  HMEDPSWITRIHERSQQMERYLSTTPETTHCRKQPRPVRIQTLVGNIHIKQEMEDDYDYYGQQRVQILER
  NESEECTEDTDQAEGTESEPKGESFDSGVSSSIGTEPDSVEQQFGAAAPRDGQAEPAQPEQAAEAPAESS
  AQPNQLEPGASSPERSNESEMDNTVITVSNSSDKGVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMP
  LTLTSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLTGQQTQFVTVSQPGLSTFTAQLPAPQPL
  ASSAGHSTASGQGDKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTG
  VRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGAR
  AGPPGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019778
• ORF Size: 3153 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019778.2, NP_062752.2
• RefSeq Size: 2990 bp
• RefSeq ORF: 2226 bp
• Locus ID: 56490
• UniProt ID: Q8K0L9
• Cytogenetics: 16 28.44 cM
• Gene Summary: May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses (By similarity). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).[UniProtKB/Swiss-Prot Function]