Mfn1 (NM_024200) Mouse Tagged ORF Clone

CAT#: MG210400

Mfn1 (tGFP-tagged) - Mouse mitofusin 1 (Mfn1)

Product Images

Circular map for MG210400

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mfn1
• Synonyms: 2310002F04Rik; 6330416C07Rik; D3Ertd265e; HR2; mKIAA4032
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210400 representing NM_024200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAACGGTATCTCCACTGAAGCACTTCGTGCTGGCAAAGAAAGCCATCACTGCAATCTTCGGCC
  AGTTACTGGAGTTTGTTACTGAGGGCTCACATTTTGTTGAAGCAACATACAGAAATCCAGAACTTGATCG
  AATAGCATCCGAGGATGATCTGGTGGAAATACAGGGCTACAGAAACAAGCTTGCTGTCATTGGGGAGGTG
  CTGTCTCGGAGACATATGAAGGTGGCATTTTTTGGCAGGACAAGTAGTGGCAAGAGCTCTGTCATCAATG
  CAATGCTGTGGGATAAAGTCCTCCCCAGCGGGATTGGTCACACAACCAACTGCTTCCTGAGTGTCGAGGG
  GACCGATGGAGATAAAGCCTACCTTATGACCGAAGGGTCAGATGAAAAGAAAAGTGTGAAGACTGTTAAT
  CAGCTGGCCCATGCCCTCCATATGGATAAAGACTTGAAAGCTGGCTGTCTTGTGCATGTATTTTGGCCCA
  AAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTTTTAGTAGACAGCCCAGGTACAGATGTCACCACAGA
  GCTGGATATCTGGATTGATAAGTTTTGCCTTGATGCTGATGTCTTTGTTTTGGTTGCAAACTCGGAATCA
  ACACTGATGAACACGGAGAAACATTTTTTCCATAAGGTGAATGAGCGGCTCTCCAAGCCCAACATCTTCA
  TTCTGAATAACCGTTGGGATGCTTCTGCTTCGGAGCCGGAGTACATGGAGGATGTGCGCAGACAGCACAT
  GGAGAGATGTCTTCACTTCTTGGTAGAAGAGCTCAAGGTTGTAAGTCCGTCGGAAGCTCGGAATCGGATC
  TTTTTTGTTTCAGCCAAGGAAGTTCTCAACTCCAGAAAGCATAAAGCTCAGGGGATGCCAGAAGGTGGTG
  GGGCACTTGCAGAAGGATTTCAAGCAAGATTACAGGAGTTTCAAAATTTTGAACAAACTTTTGAGGAGTG
  TATCTCGCAGTCAGCAGTGAAAACAAAGTTTGAACAGCACACTATCAGAGCTAAACAGATACTAGACACT
  GTGAAAAACATACTGGACTCAGTAAACGTGGCAGCAGCAGAGAAGAGGGTTTATTCAATGGAAGAGAGGG
  AAGACCAAATCGATAGACTGGACTTTATCCGAAACCAGATGAACCTTTTAACACTGGATGTTAAGAAGAA
  GATCAAGGAGGTCACGGAGGAGGTGGCAAACAAGGTTTCTTGTGCAATGACAGATGAAATTTGTCGACTA
  TCTGTTTTGGTTGATGAGTTTTGTTCTGAGTTTCATCCTACCCCCAGTGTACTGAAAGTGTATAAGAGTG
  AGTTAAATAAGCACATAGAAGATGGCATGGGAAGAAATTTGGCTGATCGGTGTACCAATGAAGTCAATGC
  CTCCATTCTTCAATCTCAGCAAGAAATCATCGAAAACTTGAAGCCACTACTTCCAGCTGGTATACAGAAT
  AAACTTCATACATTAATCCCTTGCAAAAAGTTTGACCTCAGCTATGATCTCAATTGCCACAAGCTGTGTT
  CGGATTTTCAAGAGGACATTGTGTTTCGGTTTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTCCTGGG
  TTCCACAAATGCACAGAGGGTGCTGCTCGGGCTGTCAGAGCCCATCTTTCAGGTCCCTAGATCTTTAGCT
  TCAACTCCTACTGCTCCTTCTAACCCAGCAGCCCCGGATAATGCAGCCCAGGAGGAGCTCATGATCACCC
  TGATCACAGGATTGGCGTCCCTCACGTCGAGAACCTCCATGGGCATCATCGTTGTTGGGGGCGTGATTTG
  GAAAACAGTGGGCTGGAAACTAATCTCTGTCACCTTAAGTATGTACGGAGCTCTGTACCTTTATGAGAGG
  CTGACGTGGACGACCCGTGCGAAAGAGAGAGCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAGAAGC
  TGCAGATGATTGTGAGCTTCACCAGTGCAAACTGCAGCCACCAAGTACAGCAAGAAATGGCCACTACTTT
  TGCTCGACTGTGCCAACAAGTTGATGTTACTCAGAAACATCTGGAAGAGGAAATTGCAAGATTATCCAAA
  GAGATAGACCAACTGGAGAAAATACAGAACAACTCAAAGCTCTTAAGAAATAAAGCTATTCAACTTGAAA
  GTGAGCTGGAGAATTTTTCGAAGCAGTTTCTACACCCGAGCAGTGGAGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210400 representing NM_024200
  Red=Cloning site Green=Tags(s)
  
  MAETVSPLKHFVLAKKAITAIFGQLLEFVTEGSHFVEATYRNPELDRIASEDDLVEIQGYRNKLAVIGEV
  LSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVN
  QLAHALHMDKDLKAGCLVHVFWPKAKCALLRDDLVLVDSPGTDVTTELDIWIDKFCLDADVFVLVANSES
  TLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVSPSEARNRI
  FFVSAKEVLNSRKHKAQGMPEGGGALAEGFQARLQEFQNFEQTFEECISQSAVKTKFEQHTIRAKQILDT
  VKNILDSVNVAAAEKRVYSMEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRL
  SVLVDEFCSEFHPTPSVLKVYKSELNKHIEDGMGRNLADRCTNEVNASILQSQQEIIENLKPLLPAGIQN
  KLHTLIPCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVPRSLA
  STPTAPSNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLISVTLSMYGALYLYER
  LTWTTRAKERAFKQQFVNYATEKLQMIVSFTSANCSHQVQQEMATTFARLCQQVDVTQKHLEEEIARLSK
  EIDQLEKIQNNSKLLRNKAIQLESELENFSKQFLHPSSGES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024200
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024200.2, NP_077162.1
• RefSeq Size: 2980 bp
• RefSeq ORF: 2226 bp
• Locus ID: 67414
• UniProt ID: Q811U4
• Cytogenetics: 3 15.75 cM
• Gene Summary: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:24513856, PubMed:15297672). Membrane clustering requires GTPase activity (By similarity). It may involve a major rearrangement of the coiled coil domains (PubMed:15297672). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12527753). Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity (By similarity).[UniProtKB/Swiss-Prot Function]