Mfn1 (NM_024200) Mouse Untagged Clone

CAT#: MC204514

Mfn1 (untagged) - Mouse mitofusin 1 (Mfn1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mfn1
• Synonyms: 2310002F04Rik; 6330416C07Rik; D3Ertd265e; HR2; mKIAA4032
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC056641
  GCGACCCACGCGTCCGGTGGGGAGCAGGGACGGAGTGAGTGTCCGCTGGCGGGTGGCGACGTCCGGGGTG ACCTTCGAGCTCCGGACCGGCGCAGCGCGAGCGCCCTGACGGGCCGGGGCGGCGGGCCGGGCAAGGGCGA CGGGCGGCCAGCCTGAGGGAAGGCCCTGTCCAGGTGCATAATGGCAGAAACGGTATCTCCACTGAAGCAC TTCGTGCTGGCAAAGAAAGCCATCACTGCAATCTTCGGCCAGTTACTGGAGTTTGTTACTGAGGGCTCAC ATTTTGTTGAAGCAACATACAGAAATCCAGAACTTGATCGAATAGCATCCGAGGATGATCTGGTGGAAAT ACAGGGCTACAGAAACAAGCTTGCTGTCATTGGGGAGGTGCTGTCTCGGAGACATATGAAGGTGGCATTT TTTGGCAGGACAAGTAGTGGCAAGAGCTCTGTCATCAATGCAATGCTGTGGGATAAAGTCCTCCCCAGCG GGATTGGTCACACAACCAACTGCTTCCTGAGTGTCGAGGGGACCGATGGAGATAAAGCCTACCTTATGAC CGAAGGGTCAGATGAAAAGAAAAGTGTGAAGACTGTTAATCAGCTGGCCCATGCCCTCCATATGGATAAA GACTTGAAAGCTGGCTGTCTTGTGCATGTATTTTGGCCCAAAGCAAAATGTGCCCTCTTGAGAGATGACC TGGTTTTAGTAGACAGCCCAGGTACAGATGTCACCACAGAGCTGGATATCTGGATTGATAAGTTTTGCCT TGATGCTGATGTCTTTGTTTTGGTTGCAAACTCGGAATCAACACTGATGAACACGGAGAAACATTTTTTC CATAAGGTGAATGAGCGGCTCTCCAAGCCCAACATCTTCATTCTGAATAACCGTTGGGATGCTTCTGCTT CGGAGCCGGAGTACATGGAGGATGTGCGCAGACAGCACATGGAGAGATGTCTTCACTTCTTGGTAGAAGA GCTCAAGGTTGTAAGTCCGTCGGAAGCTCGGAATCGGATCTTTTTTGTTTCAGCCAAGGAAGTTCTCAAC TCCAGAAAGCATAAAGCTCAGGGGATGCCAGAAGGTGGTGGGGCACTTGCAGAAGGATTTCAAGCAAGAT TACAGGAGTTTCAAAATTTTGAACAAACTTTTGAGGAGTGTATCTCGCAGTCAGCAGTGAAAACAAAGTT TGAACAGCACACTATCAGAGCTAAACAGATACTAGACACTGTGAAAAACATACTGGACTCAGTAAACGTG GCAGCAGCAGAGAAGAGGGTTTATTCAATGGAAGAGAGGGAAGACCAAATCGATAGACTGGACTTTATCC GAAACCAGATGAACCTTTTAACACTGGATGTTAAGAAGAAGATCAAGGAGGTCACGGAGGAGGTGGCAAA CAAGGTTTCTTGTGCAATGACAGATGAAATTTGTCGACTATCTGTTTTGGTTGATGAGTTTTGTTCTGAG TTTCATCCTACCCCCAGTGTACTGAAAGTGTATAAGAGTGAGTTAAATAAGCACATAGAAGATGGCATGG GAAGAAATTTGGCTGATCGGTGTACCAATGAAGTCAATGCCTCCATTCTTCAATCTCAGCAAGAAATCAT CGAAAACTTGAAGCCACTACTTCCAGCTGGTATACAGAATAAACTTCATACATTAATCCCTTGCAAAAAG TTTGACCTCAGCTATGATCTCAATTGCCACAAGCTGTGTTCGGATTTTCAAGAGGACATTGTGTTTCGGT TTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTCCTGGGTTCCACAAATGCACAGAGGGTGCTGCTCGG GCTGTCAGAGCCCATCTTTCAGGTCCCTAGATCTTTAGCTTCAACTCCTACTGCTCCTTCTAACCCAGCA GCCCCGGATAATGCAGCCCAGGAGGAGCTCATGATCACCCTGATCACAGGATTGGCGTCCCTCACGTCGA GAACCTCCATGGGCATCATCGTTGTTGGGGGCGTGATTTGGAAAACAGTGGGCTGGAAACTAATCTCTGT CACCTTAAGTATGTACGGAGCTCTGTACCTTTATGAGAGGCTGACGTGGACGACCCGTGCGAAAGAGAGA GCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAGAAGCTGCAGATGATTGTGAGCTTCACCAGTGCAA ACTGCAGCCACCAAGTACAGCAAGAAATGGCCACTACTTTTGCTCGACTGTGCCAACAAGTTGATGTTAC TCAGAAACATCTGGAAGAGGAAATTGCAAGATTATCCAAAGAGATAGACCAACTGGAGAAAATACAGAAC AACTCAAAGCTCTTAAGAAATAAAGCTATTCAACTTGAAAGTGAGCTGGAGAATTTTTCGAAGCAGTTTC TACACCCGAGCAGTGGAGAATCCTAACGGCAGAGGCACTGTAGGAGGAAGCGGACTTGGAAGATGGGAAA TGTTACTTTATGAAATGACCTCAGTACAAATTACTAACTCTTAGTATCGATGCCTTGCGGAGATTGTGGT AATGACCTGTCTCAGGGGTTGCACCTTTGGAAGTGTTGTGATTCGCCTTGTCTTAGCATTAGTTTGGAGT AAAGACTGAATTGTTAAGGTTAAATGATGAATTCCTTTAGAAACAGTGGAACCGGCTGTGCGGCCCCTGA GGGTGGGTCCTGCAGCTCCTCACCAGGCTGGCTGTCTGCGGCTCTCAGAAGCTGCTTCCTGGCATCCAGG AGTTAGAGACCTTTTCATCCTTTCTCAGTGCTAGTTCTTGATGCTTCTTTAATGGGAATAGTGAACTTGT TTATAAGCCGATTTGCTCAAACGAGGGGTGTGGGCTGCTCCTGGGGGGGTCCTGCAATCACTCTGTCCTC ACAGCAAGGATGTAACCACTACTAAACAGTTTTTACTTTCTTTTATTCCCATTAAAGCTGATGTGAAATA GTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_024200
• Insert Size: 2226 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC056641, AAH56641
• RefSeq Size: 2957 bp
• RefSeq ORF: 2226 bp
• Locus ID: 67414
• UniProt ID: Q811U4
• Cytogenetics: 3 15.75 cM
• Gene Summary: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:24513856, PubMed:15297672). Membrane clustering requires GTPase activity (By similarity). It may involve a major rearrangement of the coiled coil domains (PubMed:15297672). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12527753). Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity (By similarity).[UniProtKB/Swiss-Prot Function]