Ddx1 (NM_134040) Mouse Tagged ORF Clone

CAT#: MG210392

Ddx1 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1)

Product Images

Circular map for MG210392

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ddx1
• Synonyms: AA409185; DBP-RB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210392 representing NM_134040
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTTCTCCGAAATGGGTGTTATGCCGGAGATTGCACAAGCTGTGGAGGAGATGGATTGGCTTC
  TACCAACTGATATCCAGGCAGAATCTATCCCATTGATCCTAGGAGGAGGGGATGTACTCATGGCTGCAGA
  AACAGGAAGTGGAAAAACTGGTGCATTTAGTATTCCCGTTATCCAGATAGTGTATGAAACTCTGAAAGAC
  CAACAGGAAGGAAAGAAAGGGAAAACAACTATTAAAACTGGTGCTTCAGTGCTCAACAAGTGGCAGATGA
  ACCCATATGATAGAGGGTCTGCTTTTGCAATTGGATCAGATGGTCTGTGTTGTCAGAGCAGAGAAGTGAA
  GGAGTGGCATGGATGCAGAGGAACTAGAGGACTGCTGAAAGGGAAGCACTACTATGAGGTGTCCTGTCAT
  GACCAAGGGCTATGCAGAGTTGGGTGGTCTACCATGCAGGCTTCCTTAGACCTAGGTACTGACAAGTTTG
  GATTTGGCTTCGGTGGAACAGGAAAGAAATCTCATAATAAACAATTTGATAATTATGGAGAGGAATTCAC
  TATGCATGATACCATTGGATGTTACTTAGATATCGATAAAGGGCATGTGAAGTTCTCTAAGAATGGAAAA
  GATCTTGGTCTGGCATTTGAAATACCAGCACATATAAAAAACCAAGCCCTCTTCCCTGCCTGTGTTTTGA
  AGAATGCTGAATTGAAATTTAACTTTGGTGAAGAAGAATTTAAGTTTCCACCAAAAGATGGTTTTGTTGC
  TCTTTCCAAGGCCCCAGATAATTACATCGTCAAATCTCAGCACACAGGTAATGCACAGGTGTCACAAACA
  AAATTTCTCCCGAATGCTCCAAAAGCTCTCATTGTGGAACCCTCCAGAGAGTTAGCTGAGCAAACCTTGA
  ACAACGTCAAGCAGTTTAAGAAGTATATTGATAATCCTAAATTGCGGGAGCTTCTCATAATTGGTGGCGT
  TGCGGCTCGGGATCAGCTCTCTGTTCTGGATAATGGGGTTGACATTGTTGTCGGTACTCCAGGAAGATTG
  GATGATTTGGTGTCGACTGGAAAGCTCAACTTGTCTCAAGTTAGATTCCTGGTCCTGGATGAAGCAGATG
  GGCTTCTCTCTCAAGGCTACTCTGACTTCATTAACCGGATGCACAACCAGATTCCGCAGATTACGTGTGA
  TGGGAAAAGACTTCAGGTGATTGTTTGCTCTGCTACTCTGCATTCTTTTGATGTAAAGAAGCTATCGGAG
  AAGATAATGCATTTTCCCACATGGGTCGATTTGAAAGGAGAAGATTCTGTTCCAGATACTGTGCATCATG
  TCGTTGTCCCTGTGAACCCCAAAACTGACAAGCTTTGGGAAAGGCTGGGGAAGAACCACATTCGAACTGA
  TGATGTACACGCAAAGGATAACACAAGACCTGGTGCTAACAGTCCAGAGATGTGGTCTGAAGCTATTAAA
  ATACTGAAGGGGGAGTATGCTGTCCGAGCAATCAAGGAACACAAGATGGATCAAGCAATTATCTTCTGTA
  GAACTAAAATTGACTGTGATAACTTGGAGCAGTACTTTATGCAGCAAGGAGGAGGACCTGACAAAAAAGG
  ACACCAGTTCTCATGTGTATGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAACGA
  TTTAAGAAAGGAGACGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGGATTGATATCCATGGCG
  TTCCTTATGTGATCAACGTCACCCTGCCTGATGAGAAGCAGAATTATGTGCACCGGATCGGCAGAGTGGG
  ACGAGCTGAAAGGATGGGCCTGGCTATTTCCCTGGTGGCAACCGAGAAAGAAAAGGTTTGGTATCATGTA
  TGTAGCAACCGTGGGAAGGGATGCTATAACACTAGACTCAAGGAAGATGGCGGCTGTACTATCTGGTACA
  ATGAAATGCAGCTACTTTCCGAGATAGAAGAGCACCTGAACTGCACCATTTCCCAGGTCGAGCCAGATAT
  AAAGGTTCCAGTGGATGAGTTTGATGGGAAAGTCACATACGGTCAAAAAAGGGCTGCTGGCGGTGGAAAC
  TACAAAGGCCATGTGGATGTCCTGGCACCTACCGTTCAAGAGTTGGCTGCCCTTGAAAAGGAAGCACAGA
  CCTCTTTCCTACACCTTGGCTACCTTCCCAACCAGCTGTTCCGAACCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210392 representing NM_134040
  Red=Cloning site Green=Tags(s)
  
  MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKD
  QQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRGTRGLLKGKHYYEVSCH
  DQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGK
  DLGLAFEIPAHIKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDNYIVKSQHTGNAQVSQT
  KFLPNAPKALIVEPSRELAEQTLNNVKQFKKYIDNPKLRELLIIGGVAARDQLSVLDNGVDIVVGTPGRL
  DDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQITCDGKRLQVIVCSATLHSFDVKKLSE
  KIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDKLWERLGKNHIRTDDVHAKDNTRPGANSPEMWSEAIK
  ILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLER
  FKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHV
  CSNRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGN
  YKGHVDVLAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134040
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134040.1, NP_598801.1
• RefSeq Size: 2488 bp
• RefSeq ORF: 2223 bp
• Locus ID: 104721
• UniProt ID: Q91VR5
• Cytogenetics: 12 A1.1
• Gene Summary: Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation (By similarity). Acts as a positive transcriptional regulator of cyclin CCND2 expression (PubMed:19398953). Binds to the cyclin CCND2 promoter region (PubMed:19398953). Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541). Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (PubMed:21703541).[UniProtKB/Swiss-Prot Function]