Ddx1 (NM_134040) Mouse Untagged Clone

CAT#: MC200694

Ddx1 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx1
• Synonyms: AA409185; DBP-RB
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010624 sequence for NM_134040
  CGGCGTGTCTGGAAGGAGGGAATCGCCCGGGCCCGCAGCGGAGGAGTGCGGGAAGATGGCGGCCTTCTCC GAAATGGGTGTTATGCCGGAGATTGCACAAGCTGTGGAGGAGATGGATTGGCTTCTACCAACTGATATCC AGGCAGAATCTATCCCATTGATCCTAGGAGGAGGGGATGTACTCATGGCTGCAGAAACAGGAAGTGGAAA AACTGGTGCATTTAGTATTCCCGTTATCCAGATAGTGTATGAAACTCTGAAAGACCAACAGGAAGGAAAG AAAGGGAAAACAACTATTAAAACTGGTGCTTCAGTGCTCAACAAGTGGCAGATGAACCCATATGATAGAG GGTCTGCTTTTGCAATTGGATCAGATGGTCTGTGTTGTCAGAGCAGAGAAGTGAAGGAGTGGCATGGATG CAGAGGAACTAGAGGACTGCTGAAAGGGAAGCACTACTATGAGGTGTCCTGTCATGACCAAGGGCTATGC AGAGTTGGGTGGTCTACCATGCAGGCTTCCTTAGACCTAGGTACTGACAAGTTTGGATTTGGCTTCGGTG GAACAGGAAAGAAATCTCATAATAAACAATTTGATAATTATGGAGAGGAATTCACTATGCATGATACCAT TGGATGTTACTTAGATATCGATAAAGGGCATGTGAAGTTCTCTAAGAATGGAAAAGATCTTGGTCTGGCA TTTGAAATACCAGCACATATAAAAAACCAAGCCCTCTTCCCTGCCTGTGTTTTGAAGAATGCTGAATTGA AATTTAACTTTGGTGAAGAAGAATTTAAGTTTCCACCAAAAGATGGTTTTGTTGCTCTTTCCAAGGCCCC AGATAATTACATCGTCAAATCTCAGCACACAGGTAATGCACAGGTGTCACAAACAAAATTTCTCCCGAAT GCTCCAAAAGCTCTCATTGTGGAACCCTCCAGAGAGTTAGCTGAGCAAACCTTGAACAACGTCAAGCAGT TTAAGAAGTATATTGATAATCCTAAATTGCGGGAGCTTCTCATAATTGGTGGCGTTGCGGCTCGGGATCA GCTCTCTGTTCTGGATAATGGGGTTGACATTGTTGTCGGTACTCCAGGAAGATTGGATGATTTGGTGTCG ACTGGAAAGCTCAACTTGTCTCAAGTTAGATTCCTGGTCCTGGATGAAGCAGATGGGCTTCTCTCTCAAG GCTACTCTGACTTCATTAACCGGATGCACAACCAGATTCCGCAGATTACGTGTGATGGGAAAAGACTTCA GGTGATTGTTTGCTCTGCTACTCTGCATTCTTTTGATGTAAAGAAGCTATCGGAGAAGATAATGCATTTT CCCACATGGGTCGATTTGAAAGGAGAAGATTCTGTTCCAGATACTGTGCATCATGTCGTTGTCCCTGTGA ACCCCAAAACTGACAAGCTTTGGGAAAGGCTGGGGAAGAACCACATTCGAACTGATGATGTACACGCAAA GGATAACACAAGACCTGGTGCTAACAGTCCAGAGATGTGGTCTGAAGCTATTAAAATACTGAAGGGGGAG TATGCTGTCCGAGCAATCAAGGAACACAAGATGGATCAAGCAATTATCTTCTGTAGAACTAAAATTGACT GTGATAACTTGGAGCAGTACTTTATGCAGCAAGGAGGAGGACCTGACAAAAAAGGACACCAGTTCTCATG TGTATGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAACGATTTAAGAAAGGAGAC GTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGGATTGATATCCATGGCGTTCCTTATGTGATCA ACGTCACCCTGCCTGATGAGAAGCAGAATTATGTGCACCGGATCGGCAGAGTGGGACGAGCTGAAAGGAT GGGCCTGGCTATTTCCCTGGTGGCAACCGAGAAAGAAAAGGTTTGGTATCATGTATGTAGCAACCGTGGG AAGGGATGCTATAACACTAGACTCAAGGAAGATGGCGGCTGTACTATCTGGTACAATGAAATGCAGCTAC TTTCCGAGATAGAAGAGCACCTGAACTGCACCATTTCCCAGGTCGAGCCAGATATAAAGGTTCCAGTGGA TGAGTTTGATGGGAAAGTCACATACGGTCAAAAAAGGGCTGCTGGCGGTGGAAACTACAAAGGCCATGTG GATGTCCTGGCACCTACCGTTCAAGAGTTGGCTGCCCTTGAAAAGGAAGCACAGACCTCTTTCCTACACC TTGGCTACCTTCCCAACCAGCTGTTCCGAACCTTCTGACGTCATGTAGACAGAGGAGCATTGGAGTCATA AAGTCTGTAGTCTTAACTCTAACCCTCTGGTGCTGCTCTCTGAGCAGCAGTATTTACAATAACCGAGCTA TCACGGCTAACTCTCGCATGTCAGGAAACACAGACCTTAGCGATTCTCCATAAAATGGCAGCCCAATGAA AATAAACTTGACAACTATAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_134040
• Insert Size: 2223 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010624, AAH10624
• RefSeq Size: 2488 bp
• RefSeq ORF: 2223 bp
• Locus ID: 104721
• UniProt ID: Q91VR5
• Cytogenetics: 12 A1.1
• Gene Summary: Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation (By similarity). Acts as a positive transcriptional regulator of cyclin CCND2 expression (PubMed:19398953). Binds to the cyclin CCND2 promoter region (PubMed:19398953). Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541). Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (PubMed:21703541).[UniProtKB/Swiss-Prot Function]