Sarm1 (NM_172795) Mouse Tagged ORF Clone

CAT#: MG210296

Sarm1 (tGFP-tagged) - Mouse RIKEN cDNA A830091I15 gene (A830091I15Rik)

Product Images

Circular map for MG210296

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sarm1
• Synonyms: A830091I15Rik; C78606; MyD885; Sarm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210296 representing NM_172795
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGACGCTGCTCTTCTCCGCCTACAAACTGTGCCGCTTCTTCACCATGTCAGGCCCACGGCCGG
  GCGCCGATCGGCTGACAGTGCCCGGACCGGATCGGAGTGGTGGCGCCAGCCCATGGTGGGCTGCGGGCGG
  TCGCGGGTCTCGCGAAGTGTCGCCCGGAGTGGGCACTGAGGTGCAAGGCGCCCTGGAGCGTTCGCTGCCT
  GAGCTGCAGCAGGCGCTGTCCGAGCTGAAACAGGCAAGCGCGGCGCGGGCTGTGGGCGCGGGTCTCGCCG
  AGGTCTTCCAGCTGGTAGAGGAAGCCTGGCTGCTGCCGGCCGTGGGCCGCGAGGTGGCCCAAGGTCTATG
  CGATGCTATACGTCTGGACGGTGGCCTCGACTTGCTGTTGCGGCTGCTTCAGGCACCGGAGCTAGAGACC
  CGTGTGCAGGCCGCGCGCTTGCTGGAGCAGATCCTGGTGGCTGAGAACCGGGACCGCGTGGCGCGCATCG
  GTCTAGGCGTGATCTTGAACCTGGCGAAGGAGCGCGAGCCTGTGGAACTGGCACGAAGCGTGGCGGGCAT
  CTTGGAGCACATGTTCAAGCACTCGGAGGAGACGTGCCAGCGGCTGGTGGCGGCCGGAGGCCTCGACGCG
  GTGCTGTACTGGTGCCGCCGCACAGACCCGGCGCTGCTGCGCCACTGCGCTCTTGCGCTGGCGAACTGCG
  CGCTGCACGGGGGCCAGACGGTGCAGCGGTGCATGGTGGAGAAGCGCGCCGCCGAGTGGCTCTTCCCGCT
  CGCTTTCTCCAAGGAGGACGAGCTGCTGCGGCTGCACGCCTGCCTGGCGGTGGCGGTGTTGGCTACCAAC
  AAGGAGGTGGAACGCGAGGTCGAGCATTCTGGCACATTGGCGCTTGTCGAGCCGCTCGTGGCATCGCTGG
  ACCCCGGCCGCTTCGCCCGCTGCCTGGTGGATGCCAGTGACACAAGCCAGGGTCGTGGACCAGACGACCT
  GCAGAGCCTGGTGCTGTTGCTCGATTCGTCGCGTTTGGAGGCTCAGTGCATAGGAGCATTCTACCTGTGC
  GCAGAGGCTGCCATCAAGAGCCTACAGGGAAAGACCAAGGTGTTCAGCGACATCGGCGCTATCCAGAGCC
  TGAAACGCCTGGTTTCTTACTCTACGAATGGCACCACGTCGGCGCTGGCCAAGCGCGCGCTGCGCCTATT
  GGGCGAGGAGGTGCCAAGGCGCATCCTGCCCTGCGTGGCCAGCTGGAAGGAAGCTGAGGTCCAGACCTGG
  CTACAGCAGATCGGCTTCTCCCAGTACTGCGAGAACTTTCGGGAGCAGCAGGTAGATGGTGACCTGCTTC
  TAAGACTCACAGATGAAGAACTCCAGACAGACCTAGGCATGAAATCAAGCATCACCCGCAAGAGGTTCTT
  TAGGGAGCTCACAGAGCTCAAGACCTTCGCCAGCTACGCTACTTGCGACCGCAGCAACCTAGCGGACTGG
  CTGGGCAGCCTGGATCCTCGCTTCCGCCAGTACACCTATGGCCTGGTCAGCTGCGGTCTGGACCGCTCCC
  TGCTGCACCGCGTGTCAGAGCAGCAGCTCCTGGAGGACTGTGGCATCCGCCTGGGAGTGCACCGCACGCG
  CATCCTCTCTGCAGCCAGAGAAATGCTACATTCCCCGCTGCCCTGTACTGGAGGCAAGCTCAGTGGGGAC
  ACCCCAGATGTCTTTATCAGTTACCGGAGGAACTCAGGGTCCCAGCTGGCCAGCCTCCTGAAGGTGCACC
  TGCAGCTTCACGGCTTCAGCGTCTTCATCGACGTGGAGAAGCTGGAAGCCGGCAAATTCGAGGACAAGCT
  TATCCAAAGCGTCATAGCGGCTCGCAATTTTGTCCTGGTGCTGTCTGCTGGGGCGCTGGATAAGTGCATG
  CAGGACCATGACTGCAAGGACTGGGTGCACAAGGAGATTGTGACTGCTTTAAGCTGTGGCAAGAACATTG
  TGCCCATCATTGATGGCTTTGAGTGGCCTGAGCCTCAGGCGCTGCCTGAGGATATGCAGGCTGTACTCAC
  CTTCAACGGCATCAAATGGTCCCATGAGTACCAGGAGGCCACCATCGAGAAGATCATCCGCTTCCTACAG
  GGCCGCCCCTCTCAGGACTCCTCTGCCGGATCGGATACCAGTTTGGAGGGAGCTACGCCAATGGGTCTGC
  CT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210296 representing NM_172795
  Red=Cloning site Green=Tags(s)
  
  MVLTLLFSAYKLCRFFTMSGPRPGADRLTVPGPDRSGGASPWWAAGGRGSREVSPGVGTEVQGALERSLP
  ELQQALSELKQASAARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELET
  RVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDA
  VLYWCRRTDPALLRHCALALANCALHGGQTVQRCMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATN
  KEVEREVEHSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQSLVLLLDSSRLEAQCIGAFYLC
  AEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTTSALAKRALRLLGEEVPRRILPCVASWKEAEVQTW
  LQQIGFSQYCENFREQQVDGDLLLRLTDEELQTDLGMKSSITRKRFFRELTELKTFASYATCDRSNLADW
  LGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIRLGVHRTRILSAAREMLHSPLPCTGGKLSGD
  TPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVIAARNFVLVLSAGALDKCM
  QDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQALPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQ
  GRPSQDSSAGSDTSLEGATPMGLP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172795
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172795.3, NP_766383.2
• RefSeq Size: 5044 bp
• RefSeq ORF: 2175 bp
• Locus ID: 237868
• UniProt ID: Q6PDS3
• Cytogenetics: 11 46.74 cM
• Gene Summary: Negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway which plays a pivotal role in activating axonal degeneration following injury. Promotes Wallerian degeneration an injury-induced axonal death pathway which involves degeneration of an axon distal to the injury site. Can activate neuronal death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response. Inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38. Can restrict West Nile virus (WNV) pathogenesis.[UniProtKB/Swiss-Prot Function]