Tmcc2 (NM_178874) Mouse Tagged ORF Clone

CAT#: MG210162

Tmcc2 (tGFP-tagged) - Mouse transmembrane and coiled-coil domains 2 (Tmcc2)

Product Images

Circular map for MG210162

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmcc2
• Synonyms: 1110063G11Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210162 representing NM_178874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGTGCAAATCGGACGAGCTGCAGCAACAGCAGGGCGAGGAGGATGGGGCTGGGATGGAAGACG
  CTGCTTGCCTTCTGCCAGGCGCGGACCTCCGGCATGGGGAGGCCTCGAGTGCTAACTCCGCTGGCGGGCC
  AACTTCAGATGCTGGCGCTGCGGTGGCACCCAACCCGGGTCCCCGAAGCAAGCCTCCTGATTTAAAGAAA
  ATCCAGCAGCTCTCAGAGGGCTCCATGTTTGGCCACGGCCTGAAGCACCTGTTTCACAGCCGCCGCAGGT
  CACGGGAGAGGGAGCACCAGGCGTCTCAGGAGGCCCAGCAGCAGCAGCAACAGCAGGGCCTATCCGATCA
  GGACTCCCCAGATGAGAAGGAACGCTCCCCGGAGATGCACCGCGTCTCCTATGCTGTGTCCCTGCACGAC
  CTGCCTGCCCGACCTACTGCTTTCAACCGGGTGTTACAGCAAATCCGCTCCAGGCCCTCCATCAAGCGGG
  GCGCCAGCCTGCACAGCAGTGGCGGGAGCGGTGGTCGCCGTGCCAAGAGCAGCTCCCTGGAGCCACAGCG
  TGGCAGTCCTCACCTGCTTCGTAAGGCCCCCCAGGACAGCAGCCTGGCCGCCATCCTGCACCAGCACCAG
  GGCCGACCCCGTTCCTCCTCTACCACCGACACCGCCTTGCTCCTGGCCGATGGCAGCAGTGCGTACCTCC
  TGGCTGAGGAAGCAGAGAGCATCGGGGACAAGGGTGACAAGGGAGATCTTGTGGCCCTGAGCCTCCCCTC
  TGGCCCTGGCCATGGTGACTCTGATGGACCCATCAGCCTGGATGTGCCAGATGGAGCACCGGACCCCCAG
  CGAACTAAGGCTGCCATCGAACACCTGCACCAAAAGATCTTGAAGATCACTGAGCAGATCAAGATCGAGC
  AGGAGGCGCGGGATGATAACGTGGCAGAGTACCTGAAGCTGGCCAACAATGCGGACAAGCAGCAGGTGTC
  GCGCATCAAGCAGGTGTTTGAAAAGAAGAACCAGAAGTCCGCCCAGACCATCGCCCAGCTGCACAAAAAA
  CTGGAGCACTACCGTCGGCGCCTGAAGGAAATCGAGCAGAATGGGCCCTCGCGGCAGCCCAAGGATGTGC
  TACGCGATATGCAGCAAGGGCTGAAGGATGTGGGCGCCAACATGCGTGCCGGCATTAGTGGCTTCGGGGG
  TGGTGTGGTGGAGGGTGTCAAGGGCAGTCTTTCCGGCCTCTCGCAAGCTACCCACACTGCGGTGGTGTCC
  AAGCCCCGGGAGTTCGCCAGCCTCATTCGCAACAAGTTCGGCAGTGCCGACAACATCGCCCACCTGAAGG
  ACCCCATGGAAGATGGACCCCCTGAAGAGGCAGCCCGGGCGCTGAGTGGCAGTGCCACACTGGTGTCCAG
  CCCCAAGTACGGCAGTGACGATGAATGCTCGAGTGCCAGTGCCAGCTCAGCTGGGGCAGGCAGCAACTCT
  GGGGCTGGGCCAGGAGGTGCACTGGGAAGCCCGAGATCCAACACTCTTTATGGTGCCCCTGGAAATCTGG
  ACACTCTGCTGGAGGAACTGCGGGAGATAAAGGAGGGCCAGTCACACCTGGAGGACTCCATGGAGGACTT
  GAAGACTCAGCTGCAGAGGGACTACACCTACATGACCCAGTGCCTGCAAGAAGAGCGCTACAGGTATGAG
  AGGCTGGAGGAACAGCTGAACGACCTGACTGAGCTTCACCAGAATGAAATGACCAACCTAAAGCAGGAGC
  TGGCCAGCATGGAGGAGAAGGTGGCCTACCAGTCCTACGAGAGGGCCCGGGATATCCAGGAGGCCGTGGA
  GTCCTGCCTGACCCGAGTCACCAAGCTGGAACTGCAGCAGCAGCAGCAGCAAGTGGTGCAGTTAGAAGGT
  GTGGAGAACGCCAACGCGCGCGCCCTGCTGGGCAAGTTCATCAACGTGATCCTGGCACTCATGGCCGTGC
  TGCTGGTGTTTGTGTCCACCATTGCCAACTTCATCACCCCCCTCATGAAGACGCGCCTCCGGATCACCAG
  CACCGCCCTCCTGCTCCTCGTCCTCTTCCTACTGTGGAAGCACTGGGCCTCCCTCACCTACCTCCTGGAG
  CATGTGCTGCTGCCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210162 representing NM_178874
  Red=Cloning site Green=Tags(s)
  
  MKRCKSDELQQQQGEEDGAGMEDAACLLPGADLRHGEASSANSAGGPTSDAGAAVAPNPGPRSKPPDLKK
  IQQLSEGSMFGHGLKHLFHSRRRSREREHQASQEAQQQQQQQGLSDQDSPDEKERSPEMHRVSYAVSLHD
  LPARPTAFNRVLQQIRSRPSIKRGASLHSSGGSGGRRAKSSSLEPQRGSPHLLRKAPQDSSLAAILHQHQ
  GRPRSSSTTDTALLLADGSSAYLLAEEAESIGDKGDKGDLVALSLPSGPGHGDSDGPISLDVPDGAPDPQ
  RTKAAIEHLHQKILKITEQIKIEQEARDDNVAEYLKLANNADKQQVSRIKQVFEKKNQKSAQTIAQLHKK
  LEHYRRRLKEIEQNGPSRQPKDVLRDMQQGLKDVGANMRAGISGFGGGVVEGVKGSLSGLSQATHTAVVS
  KPREFASLIRNKFGSADNIAHLKDPMEDGPPEEAARALSGSATLVSSPKYGSDDECSSASASSAGAGSNS
  GAGPGGALGSPRSNTLYGAPGNLDTLLEELREIKEGQSHLEDSMEDLKTQLQRDYTYMTQCLQEERYRYE
  RLEEQLNDLTELHQNEMTNLKQELASMEEKVAYQSYERARDIQEAVESCLTRVTKLELQQQQQQVVQLEG
  VENANARALLGKFINVILALMAVLLVFVSTIANFITPLMKTRLRITSTALLLLVLFLLWKHWASLTYLLE
  HVLLPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178874
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178874.3
• RefSeq Size: 3439 bp
• RefSeq ORF: 2121 bp
• Locus ID: 68875
• UniProt ID: Q80W04
• Cytogenetics: 1 E4
• Gene Summary: May be involved in the regulation of the proteolytic processing of the amyloid precursor protein (APP) possibly also implicating APOE.[UniProtKB/Swiss-Prot Function]