Acss2 (NM_019811) Mouse Tagged ORF Clone

CAT#: MG210131

Acss2 (tGFP-tagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2)

Product Images

Circular map for MG210131

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acss2
• Synonyms: 1110017C11Rik; ACAS; Acas1; Acas2; AceCS1; Acs1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210131 representing NM_019811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTTCCCGAGGAGCGGCGCAAGAGCGGCAGTGGAAGCCGGGCTCGTGAGGAGACCGGCGCCGAGG
  GCCGGGTGCGGGGTTGGTCCCCGCCGCCGGAGGTCAGACGCTCGGCGCACGTCCCCTCTCTGCAGCGCTA
  CCGCGAGCTGCACCGGCGTTCTGTGGAGGAGCCACGGGAGTTTTGGGGAAACATTGCCAAGGAATTTTAC
  TGGAAAACTGCATGCCCTGGCCCATTCCTCCAGTACAACTTTGATGTGACTAAAGGGAAAATATTCACTG
  AGTGGATGAAAGGAGCAACTACAAACATCTGCTACAACGTGCTGGATCGAAATGTCCATGAGAAAAAACT
  TGGCGACAAAGTTGCTTTTTACTGGGAGGGCAATGAGCCAGGGGAGACCACCAAGATCACATACCGTGAA
  CTCCTGGTCCAGGTGTGTCAGTTCAGCAATGTTCTCCGTAAACAGGGCATTCAGAAGGGTGACCGAGTGG
  CCATCTACATGCCTATGATCTTGGAACTTGTGGTGGCTATGCTGGCATGTGCTCGCCTTGGAGCTTTGCA
  CTCCATTGTGTTTGCAGGCTTCTCTGCAGAGTCTCTCTGTGAAAGGATCTTGGATTCCAGTTGCTGCCTG
  CTCATCACTACAGATGCCTTCTACAGGGGGGAAAAACTTGTGAACCTGAAGGAGCTGGCTGATGAGTCCT
  TGGAGAAGTGCCGAGAGAAGGGCTTCCCAGTGAGATGCTGCATTGTGGTCAAACATCTGGGGCGGGCAGA
  GCTGGGCATGAATGACTCCCCCAGCCAGTCCCCACCAGTTAAGAGGCCATGTCCAGATGTCCAGATCTGC
  TGGAACGAAGGGGTTGACTTATGGTGGCATGAACTCATGCAGCAGGCAGGAGACGAGTGTGAGCCTGAGT
  GGTGTGATGCTGAGGACCCACTCTTCATCTTGTACACCAGTGGCTCCACAGGCAAACCTAAGGGTGTGGT
  GCACACAATTGGAGGCTATATGCTCTATGTGGCTACAACTTTCAAGTATGTGTTTGATTTCCACCCGGAA
  GATGTGTTCTGGTGCACAGCAGACATCGGCTGGATCACTGGTCATTCCTATGTCACCTATGGGCCACTGG
  CTAATGGTGCCACTAGTGTTTTGTTTGAGGGGATCCCCACATACCCAGATGAAGGGCGCTTGTGGAGCAT
  TGTGGACAAATACAAGGTGACCAAGTTCTACACGGCACCAACAGCCATCCGGATGCTCATGAAGTTTGGA
  GATGATCCTGTCACCAAGCATAGCCGGGCATCCCTGCAGGTGCTGGGCACAGTAGGTGAACCCATCAACC
  CTGAAGCCTGGCTATGGTACCACCGGGTAGTAGGTTCCCAGCGCTGCCCCATTGTAGACACCTTCTGGCA
  AACAGAAACAGGTGGCCATATGCTGACCCCTCTCCCTGGCGCCACACCCATGAAACCTGGTTCTGCTTCT
  TTCCCATTCTTCGGTGTAGCGCCTGCAATCCTGAATGAGTCCGGGGAGGAGCTGGAAGGGGAAGCTGAAG
  GTTATCTGGTGTTCAAGCAGCCCTGGCCAGGGATCATGCGCACAGTCTATGGGAACCACACACGGTTTGA
  GACCACCTACTTTAAGAAGTTCCCTGGCTACTATGTGACCGGAGATGGCTGCCGACGGGATCAGGATGGC
  TATTACTGGATCACGGGCAGGATTGATGACATGCTCAATGTGTCTGGACATCTCCTGAGTACAGCAGAGG
  TGGAATCGGCACTTGTGGAACACGAGGCTGTCGCAGAGGCAGCTGTGGTGGGCCACCCTCATCCTGTGAA
  GGGCGAATGCCTCTACTGCTTTGTTACCTTGTGTGATGGCCATACCTTCAGCCCCACACTCACAGAGGAA
  CTCAAGAAGCAGATTAGAGAAAAGATTGGCCCCATTGCCACACCAGACTACATCCAGAATGCACCTGGCT
  TGCCTAAAACACGCTCAGGGAAAATCATGAGGCGAGTTCTCCGGAAGATTGCTCAGAATGACCATGACCT
  GGGGGATACATCTACGGTGGCTGACCCATCTGTCATCAACCATCTCTTCAGTCACCGCTGCCTGACCACC
  CAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210131 representing NM_019811
  Red=Cloning site Green=Tags(s)
  
  MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFY
  WKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRE
  LLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCL
  LITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQIC
  WNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPE
  DVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFG
  DDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSAS
  FPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDG
  YYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEE
  LKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTT
  Q
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019811
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019811.2
• RefSeq Size: 2833 bp
• RefSeq ORF: 2106 bp
• Locus ID: 60525
• UniProt ID: Q9QXG4
• Cytogenetics: 2 H1
• Gene Summary: Activates acetate so that it can be used for lipid synthesis or for energy generation.[UniProtKB/Swiss-Prot Function]