Capn2 (NM_009794) Mouse Tagged ORF Clone

CAT#: MG210127

Capn2 (tGFP-tagged) - Mouse calpain 2 (Capn2)

Product Images

Circular map for MG210127

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Capn2
• Synonyms: AI326419; Capa-2; Capa2; m-calpain; m-calpin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210127 representing NM_009794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCATCGCGATAAAGCTGGCCAAGGACCGCGAAGCGGCCGAGGGGCTGGGGTCGCATGAGAGAG
  CCATCAAGTACCTCAACCAGGACTACGAGACGCTGCGGAACGAGTGCCTGGAGGCCGGGGCGCTCTTCCA
  GGATCCTTCCTTCCCCGCCCTGCCGTCATCCTTGGGCTATAAGGAGTTGGGGCCCTACTCCAGCAAAACT
  CGGGGCATCGAATGGAAGCGGCCCACGGAGATCTGCGCTGACCCCCAGTTCATTATTGGAGGAGCCACCC
  GCACAGACATCTGCCAAGGAGCCCTGGGGGACTGCTGGCTGCTGGCTGCCATCGCCTCCCTCACCTTGAA
  TGAGGAAATCCTGGCTCGGGTTGTGCCTCCAGATCAGAGCTTCCAGGAAAACTATGCAGGCATCTTCCAC
  TTCCAGTTCTGGCAATATGGCGAGTGGGTGGAGGTGGTGGTGGACGACAGGCTGCCCACTAAGGACGGGG
  AGCTGCTCTTTGTGCATTCAGCTGAAGGGAGTGAGTTCTGGAGTGCCCTTCTGGAGAAAGCCTATGCCAA
  GATCAATGGGTGCTATGAAGCACTCTCAGGGGGTGCCACCACGGAAGGCTTTGAAGACTTCACAGGAGGC
  ATTGCTGAGTGGTATGAGCTGAGGAAGCCTCCTCCCAACTTGTTCAAGATCATCCAGAAGGCTTTGGAGA
  AAGGTTCTCTGCTTGGCTGCTCTATCGATATCACCAGCGCTGCAGATTCTGAGGCCGTTACGTACCAGAA
  GTTGGTGAAAGGACATGCGTACTCTGTCACCGGAGCCGAGGAGGTTGAAAGTTCAGGAAGCCTGCAGAAA
  TTGATCCGCATCAGGAATCCCTGGGGACAAGTAGAGTGGACCGGGAAGTGGAATGACAATTGCCCCAGCT
  GGAACACGGTTGACCCAGAAGTAAGGGCAAATCTAACCGAACGGCAGGAGGACGGAGAATTCTGGATGTC
  CTTCAGTGACTTCCTGAGACACTACTCTCGCCTGGAGATCTGCAACCTGACCCCGGACACCCTCACCTGT
  GACTCCTATAAGAAGTGGAAACTCACCAAGATGGATGGGAACTGGAGGCGAGGCTCCACCGCAGGGGGCT
  GCAGGAATTACCCAAATACCTTCTGGATGAACCCTCAGTACCTAATTAAGCTGGAGGAAGAAGACGAAGA
  TGAGGAGGATGGGGAGAGAGGCTGTACCTTCCTGGTGGGTCTCATCCAGAAGCACCGGCGACGGCAGAGG
  AAGATGGGCGAGGACATGCACACCATTGGCTTCGGCATCTATGAGGTTCCAGAGGAGCTAACAGGGCAGA
  CCAACATCCACCTCGGCAAAAACTTTTTCCTCACAACCCGAGCCAGGGAGCGGTCAGATACCTTCATTAA
  CCTCCGCGAGGTCCTCAACCGCTTCAAGCTGCCCCCGGGAGAGTATGTCCTCGTTCCCTCCACCTTCGAA
  CCCCACAAGGATGGCGATTTCTGCATCCGAGTCTTCTCGGAGAAGAAGGCTGACTACCAAGCTGTTGATG
  ACGAGATCGAGGCCAACATTGAAGAGATTGACGCCAATGAGGAGGACATTGACGATGGATTCCGAAGGCT
  CTTTGTTCAGCTGGCTGGAGAGGATGCAGAGATCTCTGCCTTTGAGCTGCAGACCATCTTAAGAAGAGTT
  CTAGCCAAACGCCAAGACATCAAGTCAGACGGCTTCAGCATCGAGACCTGTAAGATCATGGTGGACATGC
  TGGATGAAGATGGGAGTGGCAAGCTTGGCCTGAAGGAGTTCTACATCCTCTGGACGAAGATTCAGAAATA
  CCAAAAAATCTACCGGGAAATCGATGTGGACAGGTCTGGAACCATGAATTCCTACGAGATGCGGAAAGCA
  CTGGAAGAAGCAGGTTTCAAGCTGCCCTGTCAACTTCATCAAGTCATCGTTGCCCGGTTTGCAGACGACG
  AGCTAATCATCGACTTTGACAATTTTGTGCGGTGTTTGGTCCGTCTGGAAACGCTATTCAAGATATTCAA
  GCAGCTAGACCCTGAGAACACTGGAACGATACAGCTCAACCTTGCTTCGTGGCTGAGTTTTTCAGTACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210127 representing NM_009794
  Red=Cloning site Green=Tags(s)
  
  MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKT
  RGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFH
  FQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGG
  IAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQK
  LIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTC
  DSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCTFLVGLIQKHRRRQR
  KMGEDMHTIGFGIYEVPEELTGQTNIHLGKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFE
  PHKDGDFCIRVFSEKKADYQAVDDEIEANIEEIDANEEDIDDGFRRLFVQLAGEDAEISAFELQTILRRV
  LAKRQDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKA
  LEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSVL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009794
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009794.4
• RefSeq Size: 3205 bp
• RefSeq ORF: 2103 bp
• Locus ID: 12334
• UniProt ID: O08529
• Cytogenetics: 1 H5
• Gene Summary: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (PubMed:22711986).[UniProtKB/Swiss-Prot Function]