Dnm1l (BC079635) Mouse Tagged ORF Clone

CAT#: MG210122

Dnm1l (tGFP-tagged) - Mouse dynamin 1-like (cDNA clone MGC:90593 IMAGE:6400313)

Product Images

Circular map for MG210122

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnm1l
• Synonyms: 6330417M19Rik; AI450666; Dlp1; Dnmlp1; Drp1; python
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210122 representing BC079635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC
  AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
  CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT
  TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGGAGTTGAAGCAGAAGAATGGGGTAAATTTC
  TTCACACCAAAAACAAGCTTTACACAGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAG
  AATTTCAGGAAATAATAAGGGGGTAAGCCCTGAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTC
  AACCTGACACTTGTGGATTTACCGGGAATGACCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGC
  TTCAGATCAGAGAACTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGC
  AAATACAGATATGGCAACATCAGAAGCACTCAAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACT
  CTAGCTGTAATCACTAAACTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGG
  TTATTCCAGTCAAGCTTGGAATAATTGGAGTAGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAG
  TGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAAT
  GGAACAAAGTATCTTGCTAGGACCCTGAATAGGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGC
  TGAAAACAAGAATAAATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGA
  TGATAAAAGTGCTACTTTACTCCAGCTTATTACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGA
  ACCGCAAAGTACATTGAAACTTCTGAGCTATGCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTT
  TCGGGCGAACCTTAGAATCTGTGGACCCACTAGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAG
  AAATGCTACTGGCCCCCGTCCTGCTTTATTTGTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAG
  ATTAAGCGTCTAGAAGAGCCCAGCCTACGGTGTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTC
  AGCATTGTAGCAATTACAGCACACAGGAATTGTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGT
  AGTGACCTGTCTTCTTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAG
  CTAGCGTATATCAACACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGG
  AACAAAGAAGAAACAGGCTAGCAAGAGAGCTGCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGG
  TGGTGGGATTGGAGACGGTGGTCAGGAACCAACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAA
  GCTGAAGAATTACTTGCTGAAGAAAAATCAAAACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCC
  ATGCTGTCAATTTGCTAGATGTGCCAGTTCCAGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTG
  TGAGGTTATTGAAAGACTTATCAAATCATATTTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCA
  AAGGCAGTAATGCATTTTTTGGTTAATCATGTGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGT
  ATAAGTCATCCTTATTAGATGACCTTCTGACTGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGC
  GGATATGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210122 representing BC079635
  Red=Cloning site Green=Tags(s)
  
  MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV
  SPEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVV
  NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRT
  LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN
  GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG
  TAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ
  IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE
  LAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAGSRDKVPSAGGGIGDGGQEPTTGNWRGMLKTSK
  AEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVP
  KAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079635
• ORF Size: 2099 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079635, AAH79635
• RefSeq Size: 3872 bp
• RefSeq ORF: 2099 bp
• Locus ID: 74006
• Cytogenetics: 16 A2
• Gene Summary: This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]