C1s (BC018319) Mouse Tagged ORF Clone

CAT#: MG210085

C1s (tGFP-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654)

Product Images

Circular map for MG210085

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C1s
• Synonyms: MGC37773, C1sa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210085 representing BC018319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAATCGCCAGGGATGTGGTGTTTGGTCCTGTTTTCTCTTTTGGCATCATTTTCTGCTGAGCCCA
  CCATGCATGGGGAGATCCTGTCCCCTAACTATCCCCAGGCGTACCCCAATGACGTCGTGAAATCTTGGGA
  CATTGAAGTCCCAGAGGGGTTTGGGATTCACCTCTACTTTACCCATGTGGACATAGAGCCGTCAGAGAGC
  TGTGCATACGACTCAGTGCAGATAATCTCAGGAGGCATCGAGGAAGGGAGACTCTGTGGCCAGAAGACCA
  GCAAGAGCCCCAACTCCCCCATTATAGAAGAGTTTCAGTTCCCATACAATAAACTTCAGGTGGTCTTTAC
  CTCAGACTTCTCCAACGAAGAACGGTTTACGGGCTTTGCAGCATACTACACTGCCATAGACATAAATGAA
  TGCACAGATTTTACAGATGTCCCCTGTAGCCACTTCTGCAATAACTTCATTGGTGGATACTTCTGCTCCT
  GTCCCCCAGAATACTTCCTCCACGATGACATGAGGAATTGTGGAGTCAACTGTAGTGGAGATGTGTTCAC
  TGCCCTGATTGGGGAGATCTCAAGTCCCAATTATCCCAATCCATACCCGGAGAACTCAAGGTGTGAATAC
  CAGATTCAGCTGCAGGAGGGCTTCCAAGTGGTGGTGACGATGCAAAGAGAAGATTTTGATGTGGAGCCTG
  CTGACTCAGAGGGGAACTGCCCTGACAGTTTAACTTTTGCTTCAAAAAACCAACAGTTTGGTCCTTACTG
  TGGTAATGGGTTCCCTGGACCGCTAACTATTAGAACCCAGAGCAATACTCTCGGCATTGTCTTTCAAACT
  GACCTAATGGGGCAAAAGAAGGGTTGGAAGCTTCGTTACCATGGAGATCCCATCTCCTGTGCCAAAAAAA
  TCACTGCCAATTCTACTTGGGAGCCCGATAAGGCAAAATACGTGTTTAAAGATGTGGTGAAGATAACCTG
  TGTGGACGGATTTGAAGTTGTGGAGGGACATGTTAGCTCTACTTCATACTATTCCACTTGTCAAAGCGAT
  GGACAGTGGAGCAACTCCGGTCTAAAATGTCAACCTGTGTATTGTGGAATTCCAGATCCCATTGCGAATG
  GTAAAGTTGAAGAGCCAGAGAATAGTGTGTTTGGTACTGTTGTTCACTACACGTGTGAAGAGCCATATTA
  CTACATGGAACATGAAGAAGGTGGGGAGTATCGATGTGCTGCAAATGGGAGATGGGTAAATGACCAGCTG
  GGTATTGAGCTTCCAAGATGTATTCCAGCCTGTGGAGTACCCACCGAGCCCTTTCAAGTGCATCAGAGGA
  TATTTGGAGGACAACCTGCAAAGATTGAAAATTTTCCCTGGCAAGTCTTCTTTAATCACCCCAGAGCTAG
  TGGGGCTCTTATTAATGAGTACTGGGTGCTGACGGCTGCTCACGTTTTGGAGAAAATCTCCGACCCTTTA
  ATGTATGTCGGGACCATGTCTGTGAGGACAACTCTCTTGGAAAATGCCCAGAGACTCTACAGTAAACGTG
  TCTTTATTCATCCCAGCTGGAAGAAAGAAGATGACCCAAACACACGGACAAATTTTGACAATGACATTGC
  CCTGGTGCAGCTGAAAGACCCTGTGAAAATGGGACCCAAAGTCTCCCCCATCTGCCTACCAGGCACCTCC
  TCAGAGTACAACGTCTCACCGGGTGACATGGGGCTGATCTCAGGGTGGGGCAGTACAGAAAAGAAAGTCT
  TTGTTATCAACCTCAGAGGGGCAAAGGTACCAGTAACCTCTTTAGAAACCTGCAAACAGGTGAAAGAGGA
  GAACCCCACTGTAAGGCCTGAGGACTATGTTTTCACTGACAACATGATCTGTGCTGGCGAGAAAGGTGTT
  GACAGCTGTCACGGGGACAGCGGCGGGGCTTTTGCTTTCCAGGTCCCCAATGTCACGGTCCCCAAATTCT
  ATGTAGCTGGCTTGGTGTCTTGGGGGAAAAGGTGTGGGACTTATGGGGTTTACACAAAGGTAAAGAATTA
  CGTGGACTGGATCCTGAAAACTATGCAGGAGAATAGTGGGCCCAGGAAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210085 representing BC018319
  Red=Cloning site Green=Tags(s)
  
  MGKSPGMWCLVLFSLLASFSAEPTMHGEILSPNYPQAYPNDVVKSWDIEVPEGFGIHLYFTHVDIEPSES
  CAYDSVQIISGGIEEGRLCGQKTSKSPNSPIIEEFQFPYNKLQVVFTSDFSNEERFTGFAAYYTAIDINE
  CTDFTDVPCSHFCNNFIGGYFCSCPPEYFLHDDMRNCGVNCSGDVFTALIGEISSPNYPNPYPENSRCEY
  QIQLQEGFQVVVTMQREDFDVEPADSEGNCPDSLTFASKNQQFGPYCGNGFPGPLTIRTQSNTLGIVFQT
  DLMGQKKGWKLRYHGDPISCAKKITANSTWEPDKAKYVFKDVVKITCVDGFEVVEGHVSSTSYYSTCQSD
  GQWSNSGLKCQPVYCGIPDPIANGKVEEPENSVFGTVVHYTCEEPYYYMEHEEGGEYRCAANGRWVNDQL
  GIELPRCIPACGVPTEPFQVHQRIFGGQPAKIENFPWQVFFNHPRASGALINEYWVLTAAHVLEKISDPL
  MYVGTMSVRTTLLENAQRLYSKRVFIHPSWKKEDDPNTRTNFDNDIALVQLKDPVKMGPKVSPICLPGTS
  SEYNVSPGDMGLISGWGSTEKKVFVINLRGAKVPVTSLETCKQVKEENPTVRPEDYVFTDNMICAGEKGV
  DSCHGDSGGAFAFQVPNVTVPKFYVAGLVSWGKRCGTYGVYTKVKNYVDWILKTMQENSGPRKD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018319
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018319.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 2084 bp
• Locus ID: 50908
• Cytogenetics: 6 F2
• Gene Summary: C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 (By similarity).[UniProtKB/Swiss-Prot Function]