Slc20a1 (NM_015747) Mouse Tagged ORF Clone

CAT#: MG209998

Slc20a1 (tGFP-tagged) - Mouse solute carrier family 20, member 1 (Slc20a1)

Product Images

Circular map for MG209998

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc20a1
• Synonyms: AI607883; Glvr-1; Glvr1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209998 representing NM_015747
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCTACTGTGGCAACGATTACTAGTACCCTAGCTGCTGTTACTGCTTCCGCTCCACCGAAGTATG
  ACAATCTATGGATGCTCATCCTGGGCTTCATCATTGCATTTGTCTTGGCATTCTCCGTGGGAGCCAATGA
  TGTAGCAAATTCGTTCGGTACAGCTGTAGGCTCAGGTGTAGTGACCCTGAAGCAAGCCTGCATCTTAGCT
  AGCATCTTCGAAACTGTGGGCTCCGCCTTGCTGGGGGCCAAAGTGAGCGAAACCATCCGGAACGGCTTGA
  TAGATGTGGAGCTGTACAACGAAACTCAAGATCTGCTCATGGCTGGCTCCGTCAGTGCTATGTTTGGTTC
  TGCTGTGTGGCAGCTCGTGGCTTCGTTTTTGAAGCTTCCGATTTCTGGGACCCATTGTATTGTCGGTGCA
  ACCATTGGTTTCTCCCTTGTGGCAAATGGGCAGAAGGGTGTCAAGTGGTCTGAACTGATAAAAATTGTGA
  TGTCGTGGTTCGTCTCTCCGCTGCTTTCTGGTATTATGTCTGGAATTTTATTCTTCCTTGTTCGTGCGTT
  CATCCTCCGTAAGGCAGATCCGGTTCCTAATGGCTTACGAGCTTTACCAATTTTTTATGCCTGCACAATC
  GGAATCAACCTCTTTTCCATTATGTATACTGGAGCACCGTTGCTGGGCTTTGACAAACTTCCTCTGTGGG
  GTACCATCCTCATCTCGGTGGGATGTGCAGTTTTCTGTGCCCTTATCGTCTGGTTCTTTGTATGTCCCAG
  GATGAAGAGAAAAATTGAACGAGAAGTAAAGTCTAGTCCATCTGAAAGTCCCTTAATGGAAAAGAAGAGC
  AACTTAAAAGAAGACCATGAAGAAACAAAGATGGCTCCTGGAGACGTTGAGCATAGGAATCCTGTGTCTG
  AGGTAGTGTGTGCCACTGGGCCACTCCGGGCTGTGGTGGAGGAGAGGACGGTGTCATTCAAACTTGGTGA
  CCTGGAGGAGGCTCCGGAGCGAGAGCGGCTTCCCATGGACCTGAAGGAGGAGACCAGCATAGACAGCACC
  ATCAATGGTGCAGTGCAGTTGCCTAATGGGAACCTTGTTCAGTTCAGTCAAACTGTCAGCAACCAGATCA
  ACTCCAGTGGCCACTATCAGTATCACACCGTGCACAAGGATTCTGGCTTGTACAAGGAGCTGCTCCATAA
  GTTACATCTGGCCAAGGTGGGAGACTGCATGGGAGATTCTGGGGACAAGCCCTTGAGACGCAACAACAGC
  TACACTTCCTACACTATGGCAATATGTGGCATGCCCCTGGATTCATTCCGTGCCAAAGAAGGTGAACAAA
  AGGGAGATGAAATGGAGACGCTGACATGGCCTAATGCAGATACCAAGAAGCGGATTCGAATGGACAGTTA
  CACCAGTTACTGCAATGCCGTGTCTGACCTTCACTCCGAGTCTGAGATGGACATGAGTGTGAAGGCTGGG
  ATGGGCCTGGGTGACAGAAAAGGAAGCAGTGGCTCTCTTGAAGAATGGTATGACCAGGATAAGCCCGAAG
  TGTCCCTTCTCTTCCAGTTCCTGCAGATCCTCACAGCCTGCTTTGGGTCATTTGCCCATGGTGGCAATGA
  CGTCAGCAATGCCATCGGCCCTCTGGTTGCTTTGTATCTTGTTTATAAACAAGAAGCCTCTACAAAAGCG
  GCAACACCCATATGGCTTCTGCTTTATGGTGGTGTTGGCATTTGCATGGGCCTGTGGGTTTGGGGAAGAA
  GAGTTATCCAGACCATGGGGAAGGACCTGACCCCAATCACACCCTCCAGTGGTTTCAGTATTGAACTGGC
  GTCTGCCTTAACTGTGGTCATCGCATCAAACATTGGCCTTCCCATCAGCACAACACATTGCAAAGTGGGC
  TCTGTTGTGTCTGTTGGCTGGCTCCGATCAAAGAAGGCTGTTGACTGGCGACTGTTTCGAAACATTTTTA
  TGGCCTGGTTTGTCACGGTCCCCATCTCTGGGGTTATCAGTGCCGCTATCATGGCAGTATTCAAGTACAT
  CATCCTGCCAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209998 representing NM_015747
  Red=Cloning site Green=Tags(s)
  
  MESTVATITSTLAAVTASAPPKYDNLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILA
  SIFETVGSALLGAKVSETIRNGLIDVELYNETQDLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGA
  TIGFSLVANGQKGVKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILRKADPVPNGLRALPIFYACTI
  GINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREVKSSPSESPLMEKKS
  NLKEDHEETKMAPGDVEHRNPVSEVVCATGPLRAVVEERTVSFKLGDLEEAPERERLPMDLKEETSIDST
  INGAVQLPNGNLVQFSQTVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNS
  YTSYTMAICGMPLDSFRAKEGEQKGDEMETLTWPNADTKKRIRMDSYTSYCNAVSDLHSESEMDMSVKAG
  MGLGDRKGSSGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYKQEASTKA
  ATPIWLLLYGGVGICMGLWVWGRRVIQTMGKDLTPITPSSGFSIELASALTVVIASNIGLPISTTHCKVG
  SVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAVFKYIILPV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015747
• ORF Size: 3313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015747.1
• RefSeq Size: 3272 bp
• RefSeq ORF: 2046 bp
• Locus ID: 20515
• UniProt ID: Q61609
• Cytogenetics: 2 62.79 cM
• Gene Summary: Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification.[UniProtKB/Swiss-Prot Function]