Mtf1 (NM_008636) Mouse Tagged ORF Clone

CAT#: MG209958

Mtf1 (tGFP-tagged) - Mouse metal response element binding transcription factor 1 (Mtf1)

Product Images

Circular map for MG209958

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mtf1
• Synonyms: MTF-1; Thyls
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209958 representing NM_008636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAACACAGTCCAGACGACAATATCATCTTCTTTAAGGGAGAGGAGGATGACCTAACCCCGCATG
  ACAAGATGCTCAGGTTTGTGGATGACAACGGACTGGTGCCTTCCTCATCTGGAACTGTTTATGATAGGAC
  CACTGTTCTCATAGAACAGGACCCTGGCACTTTGGAGGATGATGAAGACGATGGACAGTGTGGAGAACCC
  TTGCCTTTTCTGGTGGAAGGTGAAGAGGGCTTCTTAATAGATCAGGAAGCAATGTCCCAGGGTTACGTAC
  AACACATTATCTCACCAGATCAGATTCATTTGACTATAAACCCTGGTTCCACACCCATGCCAAGAAACAT
  TGAAGGTGCAACTCTTACTCTGCAGTCGGAATGTCCAGAAACGAAACGGAAAGAAGTAAAGCGGTACCAG
  TGCACCTTTGAGGGATGCCCTCGCACCTACAGCACAGCAGGCAACCTGCGCACCCACCAGAAGACTCACC
  GAGGAGAGTACACCTTCGTCTGTAATCAGGAGGGCTGTGGCAAGGCCTTCCTCACCTCCTACAGCCTCAG
  GATCCATGTGCGAGTGCACACAAAGGAGAAGCCATTTGAGTGTGACGTGCAGGGCTGTGAGAAGGCGTTC
  AACACACTGTACAGGTTGAAAGCACATCAGAGGCTTCACACAGGGAAAACGTTTAACTGTGAATCTCAAG
  GCTGCAGCAAATACTTCACCACACTCAGTGACCTGCGGAAGCACATTCGGACCCATACAGGAGAAAAGCC
  ATTTCGATGTGATCACGACGGCTGTGGGAAAGCGTTTGCAGCGAGCCACCACCTTAAAACTCACGTCCGG
  ACGCACACTGGTGAAAGACCCTTCTTCTGCCCCAGCAACGGCTGTGAGAAGACGTTCAGCACTCAGTACA
  GTCTCAAAAGTCACATGAAAGGTCATGATAACAAAGGCACTGCATACAGTGCACTTCCACAGCACAATGG
  ATCAGAGGATACAAATCACTCACTTTATCTGAGTGAGCTGGGCCTTCTGTCCACAGATTCTGAACTGCAA
  GAAAATTCCAGTTCGACACAGGACCAGGACCTCAGCACAATTTCACCAGCAATCATCTTTGAATCAATGT
  TCCAGAATTCCGACGACCCTGGGATTCAGGACGACCCTCTGCAGACAGCTGCCTTGATTGACAGTTTTAA
  TGGTGATGCAGAGTCCGTCATTGATGTCCCACCGCCTGCAGGAAATTCGGCGTCTTTATCTCTTCCACTC
  GTACTGCAGTCTGGCATCTCCGAGCCACCCCAGCCTCTGCTACCAGCAACAGCTCCGTCCGCTCCTCCAC
  CTGCTCCCTCCCTAGGACCTGGTTCCCAGCCAGCTGCATTTGGCAGCCCCCCTGCTCTCTTACAACCTCC
  AGAAGTGCCTGTTCCCCACAGCACACAGTTTGCTGCTAATCATCAAGAGTTTCTTCCGCACCCCCAGGCG
  CCACCCCAGACCATCGTACCAGGACTTTCTGTTGTTGCTGGGGCTCCTGCATCAGCAGCAACAGTGGCGT
  CAGCCGTGGCAGCACCAGCCCCACCACAAAGTACTACTGAGCCCCTGCCTGCTATGGTCCAGACTCTGCC
  CCTGGGTGCCAACTCTGTCCTAACTAATAATCCCACCATAACCATCACCCCAACTCCTAACACGGCAATC
  CTGCAGTCCAGCCTAGTCATGGGAGAACAGAACTTACAGTGGATATTGAACGGTGCCACCAGTTCACCAC
  AAAACCAAGAACAAATTCAGCAAGCATCGAAAGTGGAGCAGGTGTACTTCGCCACCGCTGTACCAGTGGC
  CAGTGGCACAGGGAGCTCTGTTCAGCAGATTGGCCTCAGTGTTCCTGTGATCATCATCAAACAAGAGGAG
  GCCTGTCAGTGTCAGTGCGCGTGCCGGGACTCTGCGAAGGAGCGGGCGGCCGGCCGGAGAAAGGGATGTT
  CTTCCCCACCCCCTCCAGAGCCGAACCCCCAGCCTCCTGATGGGCCCAGCCTGCAGCTGCCACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209958 representing NM_008636
  Red=Cloning site Green=Tags(s)
  
  MGEHSPDDNIIFFKGEEDDLTPHDKMLRFVDDNGLVPSSSGTVYDRTTVLIEQDPGTLEDDEDDGQCGEP
  LPFLVEGEEGFLIDQEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQ
  CTFEGCPRTYSTAGNLRTHQKTHRGEYTFVCNQEGCGKAFLTSYSLRIHVRVHTKEKPFECDVQGCEKAF
  NTLYRLKAHQRLHTGKTFNCESQGCSKYFTTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHVR
  THTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGTAYSALPQHNGSEDTNHSLYLSELGLLSTDSELQ
  ENSSSTQDQDLSTISPAIIFESMFQNSDDPGIQDDPLQTAALIDSFNGDAESVIDVPPPAGNSASLSLPL
  VLQSGISEPPQPLLPATAPSAPPPAPSLGPGSQPAAFGSPPALLQPPEVPVPHSTQFAANHQEFLPHPQA
  PPQTIVPGLSVVAGAPASAATVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI
  LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEQVYFATAVPVASGTGSSVQQIGLSVPVIIIKQEE
  ACQCQCACRDSAKERAAGRRKGCSSPPPPEPNPQPPDGPSLQLPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008636
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008636.2, NP_032662.2
• RefSeq Size: 5461 bp
• RefSeq ORF: 2028 bp
• Locus ID: 17764
• UniProt ID: Q07243
• Cytogenetics: 4 57.91 cM
• Gene Summary: Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).[UniProtKB/Swiss-Prot Function]