Foxred2 (NM_001017983) Mouse Tagged ORF Clone

CAT#: MG209886

Foxred2 (tGFP-tagged) - Mouse FAD-dependent oxidoreductase domain containing 2 (Foxred2)

Product Images

Circular map for MG209886

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Foxred2
• Synonyms: A430097D04Rik; D15Bwg0759e; Gm304
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209886 representing NM_001017983
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCCCTCGGGGCTGCTGGTGGCCCTAGCCTTGCACCTGGCAGTGTGTTCCCGTCCGCACCGGGACT
  ACTGCGTCCTGGGCGCCGGTCCTGCGGGCCTGCAGATGGCCGCCTTCCTGCATCGAGCCGGCAGGGACTA
  CGAGGTATTCGAACGCGAGTCCGCACCTGGCAGCTTCTTCACTCGCTACCCGCGCCACCGCAAGCTCATC
  AGCATCAACAAGCGGCACACGGGCAAGGCCAACGCCGAGTTCAACCTCCGCCACGACTGGAACTCGCTGC
  TCAGCGACGACCCACACCTGCTCTTCAGACACTACTCGCAGGCTTACTTCCCCGACGCCAGCGACATGGT
  GCGCTACCTGGGTGACTTCGCGCGGCGCCTGGGGCTCCATGTGTTATACAACACAAACATCACCCATGTG
  ACCCTGGACAAGGATCCACAAGCCTGGAACGGCCATTACTTCATTCTGACGGACCAGAAGGGCCAGGTTT
  ACCAGTGCAGCGTCCTGCTTGTAGCCACTGGTCTGGCAGTCCCCAAACTGGTTGACTTCCCCGGCTCTGA
  GTATGTGGAGGGGTACGAGTCTGTGTCCGTGGATCCTGAAGACTTTGTGGGCCAGAATGTGTTGATCCTG
  GGCCATGGGAACTCAGCCTTCGAGACGGCAGAAAACATCTTGGGTGTCACTAACTTTGTCCACATGCTGA
  GCCGTTCCCGGGTCCGGCTCTCCTGGGCCACCCACTATGTTGGAGATGTCAGAGCCATCAACAATGGCCT
  GCTGGACACCTACCAGCTGAAATCACTAGACGGGCTCCTGGAATCAGACCTGGAGTATCTGGCCCTCGTG
  AAGGACAGCAAGGGCAAGTTCCACGTCACCCTGAAATTCCTCCTGGAGAATAACAGCAGCCAGAGTGCCG
  ACTCTATTCCCCTCCCCGAGGATGACAACGACAACTTTGCCATGCGGGTGGCCTACGACCGTGTCATCCG
  CTGCCCGGGATGGACGTTTGACTTCTCCATTTTCGACCAGTCCCTCAGACTCTCCTCGGGGACTGAGTTC
  AGCAAGAAATACCCACTGATCAAAGCCAGCTATGAATCCAAAGGAAGCCGGGGTCTCTTTATCCTAGGGA
  CTGCCAGCCACTCAGTGGACTACCGGAAATCCGCTGGCGGCTTCATCCATGGATTCCGATACACAGTGCG
  TGCTGTCCACCGGCTACTGGAACATCGGCACCATGGCATCCCCTGGCCATCCACAGAGTACCCCATCACA
  CAGCTGACCAGCTCCATCATCCGGCGTGTAAATGAGGCTTCTGGGCTGTACCAGATGTTCAGTGTGCTGG
  CTGACATCATCCTGTTGAAGGAGAACGCCACAGCCTTTGAGTACCTGGAGGAGTTCCCTATGCAGATGCT
  GGCCCAGCTGGAGATGCTCACAGGGAGGACAGCACGGCATGGGCTCTTTGTCATCAACATGGAATATGGC
  AAAAACTTCTCTGGGCCTGAGAAGGATGTCTTCTATTATGACCGGTCTGTGGCACACATAGAAGATGCCT
  GGATGTCTAACTTCCTTCACCCTGTCATCTACTACTACAGACACCTCCCCACTGAGCAAGATATGCGGTT
  CCGTCCTGCACAGTGGCCCCTGCCTCGGCCTACAGCCATCCACCACATCGTGGAGGACTTCCTGACTGAC
  TGGACAGCCCCAGTAGGACACATCCTCCCATTGAGGCGCTTCTTGGAAAACTGTCTGGACACAGATCTGC
  GTAGCTTTTATGCAGAGTCCTGTTTCCTGTTCACACTCACACGCCAGAGGCTGCCGCCCTTTTGCCAGCA
  GGGATACCTGAGAATGCAGGGGCTCTCAAGCACCAAGAGCCTTTGGCAGCATGGTGTGGAAAGCAGGCTC
  CTGCAGGACTACACGGCCATGGAGAACAGCAATAGGTGGCTTGGTGACCACTCCACAGCTCCAGAGCCTC
  TGACTCAATCCCTTGACAGCAACAAAGAGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209886 representing NM_001017983
  Red=Cloning site Green=Tags(s)
  
  MGPSGLLVALALHLAVCSRPHRDYCVLGAGPAGLQMAAFLHRAGRDYEVFERESAPGSFFTRYPRHRKLI
  SINKRHTGKANAEFNLRHDWNSLLSDDPHLLFRHYSQAYFPDASDMVRYLGDFARRLGLHVLYNTNITHV
  TLDKDPQAWNGHYFILTDQKGQVYQCSVLLVATGLAVPKLVDFPGSEYVEGYESVSVDPEDFVGQNVLIL
  GHGNSAFETAENILGVTNFVHMLSRSRVRLSWATHYVGDVRAINNGLLDTYQLKSLDGLLESDLEYLALV
  KDSKGKFHVTLKFLLENNSSQSADSIPLPEDDNDNFAMRVAYDRVIRCPGWTFDFSIFDQSLRLSSGTEF
  SKKYPLIKASYESKGSRGLFILGTASHSVDYRKSAGGFIHGFRYTVRAVHRLLEHRHHGIPWPSTEYPIT
  QLTSSIIRRVNEASGLYQMFSVLADIILLKENATAFEYLEEFPMQMLAQLEMLTGRTARHGLFVINMEYG
  KNFSGPEKDVFYYDRSVAHIEDAWMSNFLHPVIYYYRHLPTEQDMRFRPAQWPLPRPTAIHHIVEDFLTD
  WTAPVGHILPLRRFLENCLDTDLRSFYAESCFLFTLTRQRLPPFCQQGYLRMQGLSSTKSLWQHGVESRL
  LQDYTAMENSNRWLGDHSTAPEPLTQSLDSNKEEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017983
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017983.1, NP_001017983.1
• RefSeq Size: 4812 bp
• RefSeq ORF: 1998 bp
• Locus ID: 239554
• UniProt ID: Q3USW5
• Cytogenetics: 15 36.92 cM
• Gene Summary: Probable flavoprotein which may function in endoplasmic reticulum associated degradation (ERAD). May bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation (By similarity).[UniProtKB/Swiss-Prot Function]