Lmna (NM_001002011) Mouse Tagged ORF Clone

CAT#: MG209880

Lmna (tGFP-tagged) - Mouse lamin A (Lmna), transcript variant 1

Product Images

Circular map for MG209880

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lmna
• Synonyms: Dhe
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209880 representing NM_001002011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCCGTCACAGCGGCGCGCCACCCGCAGTGGGGCGCAGGCCAGCTCTACCCCACTGTCGCCCA
  CTCGGATCACCCGGCTGCAGGAGAAGGAGGACCTGCAGGAGCTCAATGACCGCCTGGCCGTGTACATCGA
  TCGCGTGCGTTCCCTGGAGACCGAGAACGCGGGGCTGCGCCTTCGCATCACTGAGTCTGAAGAGGTGGTC
  AGCCGAGAGGTGTCCGGCATCAAGGCGGCCTACGAGGCCGAGCTGGGGGATGCCCGCAAGACCCTTGATT
  CTGTGGCCAAGGAGCGCGCCCGCCTCCAGCTAGAGCTGAGCAAAGTGCGTGAGGAGTTCAAGGAGCTGAA
  GGCTCGCAACACCAAGAAGGAGGGGGACTTGTTGGCTGCGCAGGCCCGGCTCAAGGACCTCGAGGCTCTT
  CTCAACTCCAAGGAAGCTGCCCTGAGCACTGCTCTCAGTGAGAAGCGCACATTGGAGGGCGAGCTCCATG
  ACCTGCGGGGGCAGGTAGCCAAGCTTGAGGCGGCCCTGGGAGAGGCTAAGAAGCAGCTTCAGGATGAGAT
  GCTGAGGCGAGTGGATGCTGAGAACAGGCTACAGACGCTGAAGGAGGAGCTTGACTTCCAGAAGAACATT
  TACAGCGAGGAACTGCGTGAGACCAAGCGCCGGCATGAGACGCGGCTTGTGGAGATCGATAACGGGAAGC
  AGCGAGAGTTTGAGAGCCGGCTGGCAGATGCCCTGCAGGAGCTGCGGGCTCAGCATGAGGACCAGGTGGA
  ACAGTATAAGAAGGAGCTAGAAAAGACATACTCCGCCAAGCTGGATAATGCCAGGCAGTCTGCTGAGAGG
  AACAGCAACCTCGTGGGGGCTGCCCATGAGGAACTGCAGCAGTCTCGAATCCGCATTGACAGCCTCTCGG
  CCCAGCTCAGCCAGCTCCAAAAGCAGTTGGCAGCCAAGGAGGCAAAGCTGCGTGACCTGGAGGACTCGCT
  GGCCCGTGAGCGCGATACCAGCCGGCGCCTGCTGGCTGAGAAAGAGCGAGAGATGGCGGAGATGCGGGCG
  AGGATGCAGCAGCAGCTGGACGAGTACCAGGAGCTGCTGGACATCAAGCTGGCCCTGGACATGGAGATCC
  ATGCCTATCGAAAGCTGCTGGAGGGCGAGGAGGAGAGGCTGCGCCTGTCCCCCAGCCCTACCTCGCAGCG
  CAGCCGTGGCCGCGCCTCCTCCCACTCATCCCAGTCTCAGGGTGGAGGCAGCGTCACCAAAAAGCGCAAG
  CTGGAGTCTTCCGAGAGCCGGAGCAGCTTCTCGCAGCATGCTCGCACTAGCGGGCGTGTGGCGGTAGAGG
  AAGTCGATGAAGAGGGAAAGTTCGTGCGGCTGCGCAACAAGTCCAACGAGGACCAGTCCATGGGCAACTG
  GCAGATCAGGCGTCAGAATGGTGACGATCCTTTGATGACCTATCGCTTCCCACCGAAGTTCACCCTAAAG
  GCTGGGCAGGTGGTGACGATCTGGGCTTCAGGAGCTGGGGCCACCCATAGCCCCCCTACTGACTTGGTGT
  GGAAGGCGCAGAACACCTGGGGCTGTGGGAGCAGCCTTCGCACCGCTCTCATCAACTCCACTGGAGAAGA
  AGTGGCCATGCGCAAGCTGGTGCGCTCACTGACCATGGTTGAGGACAATGAGGATGACGACGAGGATGGA
  GAAGAGCTCCTCCATCACCACCGTGGTTCCCACTGCAGCGGCTCGGGGGACCCCGCTGAGTACAACCTGC
  GCTCACGCACCGTGCTGTGCGGGACGTGTGGGCAGCCTGCTGACAAGGCTGCCGGTGGAGCGGGAGCCCA
  GGTGGGCGGATCCATCTCCTCTGGCTCTTCTGCCTCCAGTGTCACAGTCACTCGAAGCTTCCGCAGTGTG
  GGGGGCAGTGGGGGTGGCAGCTTCGGGGACAACCTAGTCACCCGCTCCTACCTCCTGGGCAACTCCAGTC
  CCCGGAGCCAGAGCTCCCAGAACTGCAGCATCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209880 representing NM_001002011
  Red=Cloning site Green=Tags(s)
  
  METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVV
  SREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLLAAQARLKDLEAL
  LNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTLKEELDFQKNI
  YSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAER
  NSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRA
  RMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQSQGGGSVTKKRK
  LESSESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIRRQNGDDPLMTYRFPPKFTLK
  AGQVVTIWASGAGATHSPPTDLVWKAQNTWGCGSSLRTALINSTGEEVAMRKLVRSLTMVEDNEDDDEDG
  EELLHHHRGSHCSGSGDPAEYNLRSRTVLCGTCGQPADKAAGGAGAQVGGSISSGSSASSVTVTRSFRSV
  GGSGGGSFGDNLVTRSYLLGNSSPRSQSSQNCSIM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002011
• ORF Size: 1995 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002011.3, NP_001002011.2
• RefSeq Size: 3152 bp
• RefSeq ORF: 1998 bp
• Locus ID: 16905
• UniProt ID: P48678
• Cytogenetics: 3 38.84 cM
• Gene Summary: This gene encodes a protein that is a member of the lamin family. Nuclear lamins, intermediate filament-like proteins, are the major components of the nuclear lamina, a protein meshwork associated with the inner nuclear membrane. This meshwork is thought to maintain the integrity of the nuclear envelope, participate in chromatin organization, and regulate gene transcription. Vertebrate lamins consist of two types, A and B. This protein is an A-type and is proposed to be developmentally regulated. In mouse deficiency of this gene is associated with muscular dystrophy. Mouse lines with different mutations in this gene serve as pathophysiological models for several human laminopathies. In humans, mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]