Galnt18 (NM_173739) Mouse Tagged ORF Clone

CAT#: MG209509

Galnt18 (tGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 4 (Galntl4)

Product Images

Circular map for MG209509

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Galnt18
• Synonyms: 2900011G21Rik; BC024988; Galntl4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209509 representing NM_173739
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTGTACCAGGAAGACCAAAACTTTGGTGTCCACTTGCGTGATCCTAAGCGGCATGACCAACATCA
  TATGCCTGCTGTACGTGGGCTGGGTCACCAACTACATCGCCAGCGTGTATGTGCGGGGGCAGGAGCCAGT
  GCCCGACAAGAAGCTGGAGGAAGATAAAGGGGACACCCTGAAAATTATCGAGCGGCTGGACCACTTGGAG
  AATGTCATCAAACAGCATATCCAAGAGGCTCCTGCCAAGCCTGAGGAGGCAGAGGCCGAGCCCTTCACGG
  ACTCCTCCCTGTTTGCACACTGGGGGCAGGAGCTCAGCCCGGAAGGCCGGCGTGTGGCCCTGAAGCAGTT
  CCAGTACTACGGCTACAACGCCTACCTCAGTGACCGGCTACCCCTCGACCGGCCCCTGCCAGACCTCAGA
  CCCAGTGGGTGCCGCAACCTCTCGTTCCCCGACAGCCTACCTGAGGTGAGCATCGTGTTCATCTTCGTCA
  ACGAGGCGCTCTCAGTGCTCCTGCGTTCCATCCACTCGGCCATGGAACGCACGCCATCACACCTGCTCAA
  GGAGATCATCCTGGTGGACGACAACAGCAGCAACGAGGAGCTCAAAGAAAAGCTGACTGAATATGTGGAC
  AAGGTGAACGGCCAGAAGCCAGGCTTCATCAAAGTTGTGCGGCACAGCAAGCAGGAAGGCCTCATCCGCT
  CCAGAGTCAGCGGCTGGAGGGCAGCCACAGCCCCTGTGGTGGCACTGTTCGATGCCCACGTGGAGTTCAA
  TGTGGGCTGGGCGGAACCAGTCCTCACCCGCATCAAGGAAAACCGGAAACGGATCATTTCACCATCTTTT
  GATAACATCAAATATGACAACTTTGAGATAGAAGAGTACCCGCTAGCCGCCCAAGGCTTTGACTGGGAGC
  TGTGGTGCCGCTACCTAAACCCACCCAAGGCCTGGTGGAAGCTAGAGAACTCCACAGCCCCAATCAGGAG
  CCCTGCCCTCATTGGCTGTTTCATTGTGGACCGGCAGTACTTCGAGGAGATTGGCCTGCTGGACGAAGGC
  ATGGAGGTCTATGGAGGGGAGAATGTGGAGCTTGGGATCAGGGTGAGTGAGATTTCTCACACAGGGCTGA
  GCTCAGCTCCCATGATGGTATGGCAGTGTGGCGGGAGTGTGGAGGTCCTGCCCTGCTCTCGGATTGCCCA
  CATCGAGCGAGCTCACAAGCCCTACACAGAGGACCTCACTGCCCACGTCCGCAGAAATGCCCTCAGGGTA
  GCTGAAGTCTGGATGGATGAATTCAAAAGTCATGTCTACATGGCATGGAACATACCACAAGAGGACTCAG
  GGATTGACATCGGGGACATCACCGCACGCAAGGCTCTGAGGAAGCAGCTGCAGTGCAAGACCTTCAGGTG
  GTACCTGGTCAGTGTGTACCCAGAGATGAGGATGTACTCTGACATTATCGCCTATGGAGTGCTGCAGAAT
  TCCCTGAAGACTGATTTGTGTCTTGATCAGGGCCCAGATACAGAGAATGTCCCCATCGTGTACATATGCC
  ATGGGATGACACCCCAGAATGTGTACTACACAAGCAGCCAGCAGATCCACGTGGGTATCCTGAGCCCCAC
  TGTGGATGACGATGACAACCGGTGTCTGGTGGACGTCAACAGCCGGCCACGTCTCATCGAGTGCAGTTAC
  GCCAAGGCCAAGAGGATGAAGCTGCACTGGCAGTTCTCCCAGGGAGGCTCCATCCAGAACCGCAAGTCCA
  AGCGCTGCCTGGAGCTGCAAGAGAACAGTGACATGGAGTTTGGTTTCCAGCTGGTGCTTCAGAAGTGCTC
  GGGACAACACTGGACTATCACCAATGTCCTGAGGAGCCTGGTGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209509 representing NM_173739
  Red=Cloning site Green=Tags(s)
  
  MVCTRKTKTLVSTCVILSGMTNIICLLYVGWVTNYIASVYVRGQEPVPDKKLEEDKGDTLKIIERLDHLE
  NVIKQHIQEAPAKPEEAEAEPFTDSSLFAHWGQELSPEGRRVALKQFQYYGYNAYLSDRLPLDRPLPDLR
  PSGCRNLSFPDSLPEVSIVFIFVNEALSVLLRSIHSAMERTPSHLLKEIILVDDNSSNEELKEKLTEYVD
  KVNGQKPGFIKVVRHSKQEGLIRSRVSGWRAATAPVVALFDAHVEFNVGWAEPVLTRIKENRKRIISPSF
  DNIKYDNFEIEEYPLAAQGFDWELWCRYLNPPKAWWKLENSTAPIRSPALIGCFIVDRQYFEEIGLLDEG
  MEVYGGENVELGIRVSEISHTGLSSAPMMVWQCGGSVEVLPCSRIAHIERAHKPYTEDLTAHVRRNALRV
  AEVWMDEFKSHVYMAWNIPQEDSGIDIGDITARKALRKQLQCKTFRWYLVSVYPEMRMYSDIIAYGVLQN
  SLKTDLCLDQGPDTENVPIVYICHGMTPQNVYYTSSQQIHVGILSPTVDDDDNRCLVDVNSRPRLIECSY
  AKAKRMKLHWQFSQGGSIQNRKSKRCLELQENSDMEFGFQLVLQKCSGQHWTITNVLRSLVS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173739
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173739.4
• RefSeq Size: 2595 bp
• RefSeq ORF: 1869 bp
• Locus ID: 233733
• UniProt ID: Q8K1B9
• Cytogenetics: 7 E3- F1
• Gene Summary: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.[UniProtKB/Swiss-Prot Function]