Dtx4 (NM_172442) Mouse Tagged ORF Clone

CAT#: MG209443

Dtx4 (tGFP-tagged) - Mouse deltex 4 homolog (Drosophila) (Dtx4)

Product Images

Circular map for MG209443

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dtx4
• Synonyms: AI449438; BC044798; D930042H13; RNF155
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209443 representing NM_172442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGGCCTCGGCAGTGGTGGTGTGGGAATGGCTGAACGAACACGGCCGCTGGCGTCCCTACAGCC
  CAGCCGTGAGTCACCACATCGAGGCGGTGGTGCGCGCTGGTCCCCGCGCCGGGGGCAGCGTGGTGTTGGG
  CCAGGTGGACAGCCGGCTCGCGCCCTACATCATCGACCTGCAGTCCATGAACCAGTTCCGCCAAGACACG
  GGGACCCTCCGCCCAGTTCGACGAAACTACTATGACCCATCCTCAGCCCCTGGTAAGGGAGTCGTGTGGG
  AATGGGAGAATGACAATGGTTCCTGGACTCCCTATGACATGGAAGTCGGCATCACCATCCAGTATGCCTA
  TGAGAAGCAGCACCCATGGATCGACCTCACCTCCATTGGCTTCAGCTACATAATAGACTTCAGCACCATG
  GGCCAGATCAACAGGCAGACCCAACGCCAGCGCCGTGTCAGACGACGACTAGACCTCATCTACCCCATGG
  TCACGGGCACTATGCCAAAGACTCAGTCCTGGCCAGTCAGTCCTGGACCGGCGACGTCCTCCCCTGCACC
  ACCTTGCTCCTGTCCACAGTGTGTTTTGGTGATGAGCGTCAAGGCTGCTGTGGTCCATGGGGGCACTGGG
  CCTCCAGCAGTACGAAAGAACATGGCCCTCTCTGGAGTGGGCAAGCTGCCCCAGCCACCTGGCCCTGGAG
  CAAAGCCACTAGACACCACTGGCACCATTCGAGGCCCAGGGAAGACAGCCCCATCGCAGGTTATCAGAAG
  ACAAGTCTCTAATGCACCAGCTGGGGCGACTGTAGGCTCCCCTACCAGCCCACAAGGAAGCAACAGGAAG
  ACTGGAAGGGTGGCACTGGCCACTTTGAATCGGTCCAATCTGCAGCGACTGGCCATTGCCCAGTCCCGGG
  TGCTGATTGCCTCCGGGGTTCCCACTGTGCCTGTGAAAAACTTGAATGGGTCCAGCCCTGTTAATCCTGC
  CTTGGCAGGAATCACCGGGATACTCATGAGTGCAGCGGGATTGCCTGTGTGCCTCACTCGCCCACCAAAG
  CTGGTCCTCCACCCGCCCCCTGTCAGCAAGAGTGAGATAAAGTCCATCCCAGGGGTCTCGAACACGAGCC
  GCAAGACCACCAAAAAGCAAGCCAAGAAAGGTAAAACCCCAGAGGAAGTGCTAAAGAAGTACCTGCAGAA
  GGTCCGGCACCCACCAGAAGAGGATTGTACCATCTGTATGGAGCGTCTCACAGCCCCCTCTGGCTACAAA
  GGCCCACAGCCTACAGTCAAGCCTGACCTGGTGGGAAAGCTGTCCAGGTGTGGCCACATCTACCACATCT
  ACTGCCTGGTGGCTATGTACAACAATGGGAACAAGGATGGGAGTTTACAGTGCCCAACATGTAAGACCAT
  TTATGGCGTCAAGACAGGCACCCAGCCTCCAGGGAAGATGGAATACCACCTCATCCCCCACTCCTTGCCT
  GGCCATCCGGACTGCAAGACCATCCGGATCATCTACAGTATCCCCCCTGGCATTCAGGGACCAGAGCATC
  CGAATCCTGGGAAGAGCTTCAGTGCCCGTGGCTTCCCACGACATTGTTACCTTCCAGACAGCGAGAAGGG
  GAGAAAAGTTCTGAAGCTGCTTCTAGTGGCCTGGGACCGCCGTCTCATCTTTGCCATTGGAACCTCCAGC
  ACCACGGGCGAGTCCGATACTGTCATCTGGAATGAGGTTCACCACAAAACAGAATTTGGCTCTAATCTCA
  CTGGCCATGGCTACCCAGATGCCAATTACCTGGATAACGTGCTGGCTGAACTGGCTGCCCAGGGTATCTC
  TGAAGACAGCACTTCCCATGAAAAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209443 representing NM_172442
  Red=Cloning site Green=Tags(s)
  
  MLLASAVVVWEWLNEHGRWRPYSPAVSHHIEAVVRAGPRAGGSVVLGQVDSRLAPYIIDLQSMNQFRQDT
  GTLRPVRRNYYDPSSAPGKGVVWEWENDNGSWTPYDMEVGITIQYAYEKQHPWIDLTSIGFSYIIDFSTM
  GQINRQTQRQRRVRRRLDLIYPMVTGTMPKTQSWPVSPGPATSSPAPPCSCPQCVLVMSVKAAVVHGGTG
  PPAVRKNMALSGVGKLPQPPGPGAKPLDTTGTIRGPGKTAPSQVIRRQVSNAPAGATVGSPTSPQGSNRK
  TGRVALATLNRSNLQRLAIAQSRVLIASGVPTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPK
  LVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKKGKTPEEVLKKYLQKVRHPPEEDCTICMERLTAPSGYK
  GPQPTVKPDLVGKLSRCGHIYHIYCLVAMYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLP
  GHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSS
  TTGESDTVIWNEVHHKTEFGSNLTGHGYPDANYLDNVLAELAAQGISEDSTSHEKD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172442
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172442.2, NP_766030.2
• RefSeq Size: 5243 bp
• RefSeq ORF: 1851 bp
• Locus ID: 207521
• UniProt ID: Q6PDK8
• Cytogenetics: 19 A
• Gene Summary: Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4 (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.[UniProtKB/Swiss-Prot Function]