Hirip3 (NM_172746) Mouse Tagged ORF Clone

CAT#: MG209311

Hirip3 (tGFP-tagged) - Mouse HIRA interacting protein 3 (Hirip3)

Product Images

Circular map for MG209311

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hirip3
• Synonyms: B130036O03; C86302
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209311 representing NM_172746
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGGAGATGAGATGCGAAAATTCACTCGCAGCTTCTTTCGAGCTTGCCCGGACCTCAGCTCGG
  TTACGCACGCCATCGTGCGACAGAAATTTTTGATTCACGTGGGTCGGGATCATCTTGAACCTGAAGAGAA
  GCAGGCGTTGAAGCGGCTGGTGGAGGAGGAGCTGCCGAAAATGCAGGCGGATGCTGGTACCAGGGAAGGA
  AAGCCAGACTTTATCAAAGTGAAGAGGTCTCCTGCTCCCTGCAGTGACCCAAAGAAGAAAAGGTTCCGCT
  TTAATTCAGAGTCAGAATCCAGCTCTTCACCATCCAGTCCAGATGGCTCAGGACCCTCAACAAAGAACAG
  GACAACCAAGAAGACATGTCTAAGAAGAGCCTTAAAGAAAGCAGTTGAGAGCACAGATGAAGACCACCAA
  ACAGACCTGGATGCAAAGATGGGATTGGAGGAGAGCAGTGAGGGGGAAGCAGAGGGCTCTGTTAGATCAG
  GTAAGGTCACCGAGGAAGAGGAGGACATGAAACAAGAACAGAAGGGCCAGGTTAGAAAGCAAGCTGGGGC
  CAAAGACAAGCAAGTACCACTCAAGGCAGACAGGAAGCAGGTTAGGGAAGAGAGTGGGAGCAGTGAGGAG
  GAGGCTGTGCTGCAGAGAGCAAAGGTGGAGGGAAGCACAGGTGCTAACTGCCAGGAGGAGAGTGAAGAGA
  GCGGAGAGGAGAGCCCAGCTAAGAAGAAAGAGCTTAGTGAGCCCAGATCCAGAAGTAACAGAGCAGAGAG
  AACAGCCAGGGAGAGAAAGAGCTATAAGCAGAAAAGCAGGCCTGGGAGGCCAACTGGAGGCTTGCGAGAC
  AGCGAGGCAGAGAAGGAAGGGGGCACAGTGGGCAGTGGGGACAGCAGTGAGGAGGGGGAGGCAGAGAAGG
  AAGGAGGCACAGTGGGCAGTGGGGACAGCAGTGAGAAGGGGGAGGCAGAGAAGGAAGAGGGCACAGTGGG
  TAGTGGGGACAGCAGTGAGGAGGGAGAAGAGCACTCGGTGAAGAGAAAGAGCAAGGTCAGGACCCAGACC
  GAGAGTGGGAGGAGGCAGAACACAAGCAGCAGGGATGACAGTAACAGCACACAGGAGCAAGCAGCTGCTC
  AAGGCACTACAAAGTCAGGCAGCCTAGGCAGCAGTAATGGTGACAGTGACACAGAGAGGGAAGTGAGTGA
  CTCCCAGGCAGGACAAAACACCAAAGAGGAGAGGAAGAGCCGCTCTTCCAACAAGAGCTCCAAGAACGGC
  CAGGCCCGAAGTTGTTCATCTTCATCAGATTCGAGTCCAGAACCCACAGGTCAGAAGGGCAAGGCCCGAA
  GTTCCTCATCTTCATCAGATTCAGGTCCAGAACCCACAGGTCGGAAGGCTGCCTCTCGCTGTGGAGAGGA
  CCACCCAGCTGTGGCTAGACTAAAGCGTTACATTCGAGCCTGTGGTGCCCACCGAAACTACAAGAAGCTG
  CTGGGCTCCTGCAGCTCCCACAAGGCGCGCCTGAGTGTCCTCAGGGCTGAGCTGGAAGCACTGGGCATGA
  AGGGGAACCCTTCCTTGGAGAAGTGTCGCGCTCTGAAGCTGCAGCGGGAAGAGGCAGCTGAAGTGGCTGC
  CCTGGATGTTGCCAACATCATCAGCAGTACAGGCCGGCCACGAAGACGAAATGCCTGGAACCCTTCAGGG
  GAAGGGACCTCCCCAGGAGAGACTTATCGCCGAACCCTGGACTCAGAAGAGGAGCAGCCGCGCCAAGCAC
  CTCCAGACTGGTCCCATATGCGTGATATCATCAGCAGTGACGGTGACAGCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209311 representing NM_172746
  Red=Cloning site Green=Tags(s)
  
  MAPGDEMRKFTRSFFRACPDLSSVTHAIVRQKFLIHVGRDHLEPEEKQALKRLVEEELPKMQADAGTREG
  KPDFIKVKRSPAPCSDPKKKRFRFNSESESSSSPSSPDGSGPSTKNRTTKKTCLRRALKKAVESTDEDHQ
  TDLDAKMGLEESSEGEAEGSVRSGKVTEEEEDMKQEQKGQVRKQAGAKDKQVPLKADRKQVREESGSSEE
  EAVLQRAKVEGSTGANCQEESEESGEESPAKKKELSEPRSRSNRAERTARERKSYKQKSRPGRPTGGLRD
  SEAEKEGGTVGSGDSSEEGEAEKEGGTVGSGDSSEKGEAEKEEGTVGSGDSSEEGEEHSVKRKSKVRTQT
  ESGRRQNTSSRDDSNSTQEQAAAQGTTKSGSLGSSNGDSDTEREVSDSQAGQNTKEERKSRSSNKSSKNG
  QARSCSSSSDSSPEPTGQKGKARSSSSSSDSGPEPTGRKAASRCGEDHPAVARLKRYIRACGAHRNYKKL
  LGSCSSHKARLSVLRAELEALGMKGNPSLEKCRALKLQREEAAEVAALDVANIISSTGRPRRRNAWNPSG
  EGTSPGETYRRTLDSEEEQPRQAPPDWSHMRDIISSDGDSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172746
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172746.3, NP_766334.1
• RefSeq Size: 2511 bp
• RefSeq ORF: 1806 bp
• Locus ID: 233876
• UniProt ID: Q8BLH7
• Cytogenetics: 7 F3
• Gene Summary: May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.[UniProtKB/Swiss-Prot Function]