Lsr (NM_017405) Mouse Tagged ORF Clone

CAT#: MG209253

Lsr (tGFP-tagged) - Mouse lipolysis stimulated lipoprotein receptor (Lsr)

Product Images

Circular map for MG209253

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lsr
• Synonyms: ILDR3; Lisch7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209253 representing NM_017405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGCGGCCAGCGCGTGTGCTGGGGCGCCTGGCTCCCACCCGGCCACCACGATCTTCGTGTGTC
  TTTTTCTCATCATTTACTGCCCAGACCGTGCCAGTGCCATCCAGGTGACCGTGCCTGACCCCTACCACGT
  AGTGATCCTGTTCCAGCCAGTGACACTACACTGCACCTACCAGATGAGCAATACCCTCACAGCCCCTATC
  GTGATCTGGAAGTATAAGTCGTTCTGTCGGGACCGTGTTGCCGACGCCTTCTCCCCTGCCAGCGTGGACA
  ACCAGCTCAACGCCCAGCTGGCGGCTGGCAACCCCGGCTACAACCCCTATGTGGAGTGCCAGGACAGCGT
  ACGCACTGTCAGGGTGGTGGCCACCAAACAGGGCAATGCTGTGACCCTGGGAGACTACTACCAGGGCAGG
  AGAATCACCATCACAGGAAATGCTGACCTGACCTTCGAGCAGACGGCCTGGGGAGACAGTGGAGTGTATT
  ACTGCTCCGTGGTCTCAGCCCAAGATCTGGATGGGAACAACGAGGCGTACGCAGAGCTCATTGTCCTTGG
  CAGGACCTCAGAAGCCCCTGAGCTCCTACCTGGTTTTCGGGCGGGGCCCTTGGAAGATTGGCTCTTTGTG
  GTCGTGGTCTGCCTGGCAAGCCTCCTCTTCTTCCTCCTCCTGGGCATCTGCTGGTGCCAGTGCTGTCCCC
  ACACCTGCTGCTGCTATGTCAGATGTCCCTGCTGCCCAGACAAGTGCTGTTGCCCTGAGGCCCTTTATGC
  TGCTGGCAAAGCAGCCACCTCAGGTGTGCCAAGCATCTATGCCCCCAGCATCTATACCCACCTCTCTCCT
  GCCAAGACTCCGCCACCTCCGCCTGCCATGATTCCCATGCGTCCTCCCTATGGGTACCCTGGAGACTTTG
  ACAGGACCAGCTCAGTTGGTGGCCACAGCTCCCAGGTGCCCCTGCTGCGTGAAGTGGATGGGAGCGTATC
  TTCAGAAGTACGAAGTGGCTACAGGATCCAGGCTAACCAGCAAGATGACTCCATGAGGGTCCTATACTAT
  ATGGAGAAGGAGCTAGCCAACTTCGATCCTTCCCGGCCTGGCCCTCCCAATGGCCGAGTGGAACGGGCCA
  TGAGTGAAGTAACCTCCCTCCATGAAGATGACTGGCGATCTCGGCCTTCCAGGGCTCCTGCCCTCACACC
  CATCAGGGATGAGGAGTGGAATCGCCACTCCCCTCGGAGTCCCAGAACATGGGAGCAGGAACCCCTTCAA
  GAACAGCCAAGGGGTGGTTGGGGGTCTGGGCGGCCTCGGGCCCGCTCTGTGGATGCTCTAGATGACATCA
  ACCGGCCTGGCTCCACTGAATCAGGAAGGTCTTCTCCCCCAAGTAGTGGACGGAGAGGGCGGGCCTATGC
  ACCTCCGAGAAGTCGCAGCCGGGATGACCTCTATGACCCCGACGATCCTAGAGACTTGCCACATTCCCGA
  GATCCCCACTATTATGATGATTTGAGGTCTAGGGATCCACGTGCTGACCCCAGATCCCGTCAGCGATCCC
  ACGATCCTCGGGATGCTGGCTTCAGGTCACGGGACCCTCAGTATGATGGGCGACTCTTAGAAGAGGCTTT
  AAAGAAAAAAGGGGCTGGGGAGAGAAGACGCGTTTACAGGGAGGAAGAAGAAGAAGAAGAGGAGGGCCAC
  TATCCCCCAGCACCTCCGCCTTACTCTGAGACTGACTCGCAGGCCTCGAGGGAGCGGAGGATGAAAAAGA
  ATTTGGCCCTGAGTCGGGAAAGTTTAGTCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209253 representing NM_017405
  Red=Cloning site Green=Tags(s)
  
  MAPAASACAGAPGSHPATTIFVCLFLIIYCPDRASAIQVTVPDPYHVVILFQPVTLHCTYQMSNTLTAPI
  VIWKYKSFCRDRVADAFSPASVDNQLNAQLAAGNPGYNPYVECQDSVRTVRVVATKQGNAVTLGDYYQGR
  RITITGNADLTFEQTAWGDSGVYYCSVVSAQDLDGNNEAYAELIVLGRTSEAPELLPGFRAGPLEDWLFV
  VVVCLASLLFFLLLGICWCQCCPHTCCCYVRCPCCPDKCCCPEALYAAGKAATSGVPSIYAPSIYTHLSP
  AKTPPPPPAMIPMRPPYGYPGDFDRTSSVGGHSSQVPLLREVDGSVSSEVRSGYRIQANQQDDSMRVLYY
  MEKELANFDPSRPGPPNGRVERAMSEVTSLHEDDWRSRPSRAPALTPIRDEEWNRHSPRSPRTWEQEPLQ
  EQPRGGWGSGRPRARSVDALDDINRPGSTESGRSSPPSSGRRGRAYAPPRSRSRDDLYDPDDPRDLPHSR
  DPHYYDDLRSRDPRADPRSRQRSHDPRDAGFRSRDPQYDGRLLEEALKKKGAGERRRVYREEEEEEEEGH
  YPPAPPPYSETDSQASRERRMKKNLALSRESLVV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017405
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017405.2
• RefSeq Size: 1982 bp
• RefSeq ORF: 1785 bp
• Locus ID: 54135
• UniProt ID: Q99KG5
• Cytogenetics: 7 B1
• Gene Summary: Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity).[UniProtKB/Swiss-Prot Function]