Acot11 (NM_025590) Mouse Tagged ORF Clone

CAT#: MG209248

Acot11 (tGFP-tagged) - Mouse acyl-CoA thioesterase 11 (Acot11)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acot11
• Synonyms: 1110020M10Rik; 2010309H15Rik; AW060409; BFIT; BFIT1; mKIAA0707; Thea; Them1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209248 representing NM_025590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCAGAATGTGGGCAACCACTTGCGAAGGGGCTTCGCCTCTATGTTCTCTAATCGCACATCCCGGA
  AGTCAATCTCCCATCCGGAGTCTGGAGACCCTCCTACCATGGCAGAGGGTGAAGGATACCGGAACCCCAC
  GGAGGTGCAGATGAGCCAGCTGGTACTGCCCTGCCACACCAACCACCGTGGGGAGCTGAGCATTGGACAG
  TTGCTCAAGTGGATCGACACCACAGCCTGCCTATCAGCGGAGAGGCATGCTGGCTGTCCCTGCGTCACAG
  CCTCTATGGATGACATCTACTTCGACCATACCATTAGTGTCGGCCAAGTGGTGAATATCAAGGCCAAGGT
  GAACCGGGCCTTCAACTCCAGCATGGAGGTGGGAATCCAGGTGGTCTCTGAGGATCTGTGCTCTGAGAAG
  CAGTGGAGTGTCTGCAAGGCCTTGGCCACCTTTGTGGCCCACCGGGAGCTCTCCAAGGTGAAGCTGAAGC
  AGGTCATCCCATTGACCGAGGAGGAGAAGACTGAACATGGGGTGGCGGCTGAGCGCCGGCGTATGCGACT
  GGTCTATGCAGACACCATCAAAGATCTCCTAACCCACTGTGTCATCCAGGACGATTTGGACAAGGACTGC
  AGCAATATGGTGCCAGCCGAGAAGACCCGAGTGGAGAGTGTGGAGCTGGTGCTGCCTCCTCACGCCAATC
  ATCAGGGCAATACCTTCGGGGGACAGATCATGGCTTGGATGGAGAATGTGGCCACCATTGCAGCCAGCCG
  GCTCTGTCACGCCCACCCTACGCTCAAGGCCATCGAGATGTTCCATTTCCGAGGCCCGTCTCAGGTGGGG
  GACCGTCTGGTGCTCAAGGCCATCGTGAATAACGCCTTTAAGCACAGCATGGAGGTGGGTGTGTGTGTGG
  AGGCGTACCGCCAGGAAGCTGAGACCCAGCGCCGGCACATCAACAGCGCCTTCATGACCTTCGTGGTCCT
  GGACAAAGATGACCAGCCTCAGAAGCTGCCCTGGATTCGTCCCCAGCCTGGAGAGGGTGAACGGCGATAC
  CGAGAAGCCAGTGCCAGGAAGAAGATCCGCCTGGACAGGAAATACCTTGTGTCCTGTAAGCAGGCAGAAG
  TGGCCCTGTCTGTCCCCTGGGACCCTAGCAACCAGGTATACCTGAGCTACTACAACGTGTCCTCTCTGAA
  GACGCTCATGGCCAAGGACAACTGGGTGCTGTCCGTGGAGATCAGCGAGGTCCGCCTGTACATCCTAGAA
  GAGGACTTCCTCTCCTTTCACTTGGAGATGGTGGTAAATGTGGATGCCGCCCAGGTCTTTCAGCTGCTGT
  CAGACCTGCGCAGGAGACCAGAGTGGGACAAGCATTACCGGAGTGTGGAGCTGGTGCAGCAAGTGGATGA
  GGATGACGCCATCTACCACGTCATCAGCCCCGCCCTGAGCGGGAACACCAAGCCCCAGGACTTTGTGATC
  CTGGCCTCTAGGCGGAAGCCTTGTGACAATGGGGACCCCTATGTCATTGCCCTGAGGTCGGTCACGCTGC
  CCACGCACCATGAGACACCGGAATACCAACGTGGGGAGACTCTCTGTTCAGGCTTCTGTCTGTGGCGTGA
  GGGGGACCAGATGACTAAGGTTTCTTACTACAACCAGGCCACCCCCGGCTTTCTCAACTATGTGACCACC
  AATGTGTCCGGCCTGTCCTCAGAATTCTACAACACTTTCAAGGCTTGTGAGAGTTTTCTGTTGGACAACC
  GGAATGACCTAGCTCCCAGCCTCCAGACCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209248 representing NM_025590
  Red=Cloning site Green=Tags(s)
  
  MIQNVGNHLRRGFASMFSNRTSRKSISHPESGDPPTMAEGEGYRNPTEVQMSQLVLPCHTNHRGELSIGQ
  LLKWIDTTACLSAERHAGCPCVTASMDDIYFDHTISVGQVVNIKAKVNRAFNSSMEVGIQVVSEDLCSEK
  QWSVCKALATFVAHRELSKVKLKQVIPLTEEEKTEHGVAAERRRMRLVYADTIKDLLTHCVIQDDLDKDC
  SNMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAASRLCHAHPTLKAIEMFHFRGPSQVG
  DRLVLKAIVNNAFKHSMEVGVCVEAYRQEAETQRRHINSAFMTFVVLDKDDQPQKLPWIRPQPGEGERRY
  REASARKKIRLDRKYLVSCKQAEVALSVPWDPSNQVYLSYYNVSSLKTLMAKDNWVLSVEISEVRLYILE
  EDFLSFHLEMVVNVDAAQVFQLLSDLRRRPEWDKHYRSVELVQQVDEDDAIYHVISPALSGNTKPQDFVI
  LASRRKPCDNGDPYVIALRSVTLPTHHETPEYQRGETLCSGFCLWREGDQMTKVSYYNQATPGFLNYVTT
  NVSGLSSEFYNTFKACESFLLDNRNDLAPSLQTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025590
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025590.4, NP_079866.2
• RefSeq Size: 5668 bp
• RefSeq ORF: 1785 bp
• Locus ID: 329910
• UniProt ID: Q8VHQ9
• Cytogenetics: 4 C7
• Gene Summary: Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway.[UniProtKB/Swiss-Prot Function]