Gad1 (NM_008077) Mouse Tagged ORF Clone

CAT#: MG209243

Gad1 (tGFP-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1)

Product Images

Circular map for MG209243

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gad1
• Synonyms: EP1; EP10; Gad-; Gad-1; GAD2; GAD4; GAD6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209243 representing NM_008077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTTCCACTCCTTCGCCTGCAACCTCCTCGAACGCGGGAGCGGATCCTAATACTACCAACCTGC
  GCCCTACAACGTATGATACTTGGTGTGGCGTAGCCCATGGATGCACCAGAAAACTGGGCCTGAAGATCTG
  TGGCTTCTTACAAAGGACCAATAGCCTGGAAGAGAAGAGTCGTCTTGTGAGCGCCTTCAGGGAGAGGCAG
  TCCTCCAAGAACCTGCTTTCCTGTGAAAACAGTGACCAGGGTGCCCGCTTCCGGCGCACAGAGACCGACT
  TCTCCAACCTGTTTGCTCAAGATCTGCTTCCAGCTAAGAACGGGGAGGAGCAAACTGCGCAGTTCTTGCT
  GGAAGTGGTAGACATACTCCTCAACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTTCTGGATTTC
  CACCACCCACACCAGTTGCTGGAAGGCATGGAAGGCTTTAATTTGGAGCTGTCTGACCACCCCGAGTCTC
  TGGAGCAGATCCTGGTTGACTGTAGAGACACCCTGAAGTACGGGGTTCGCACAGGTCACCCTCGATTTTT
  CAACCAGCTCTCTACTGGTTTGGATATCATTGGTTTAGCTGGTGAATGGCTGACATCGACTGCCAATACC
  AATATGTTCACATATGAAATTGCACCCGTGTTTGTTCTCATGGAACAGATTACTCTTAAGAAGATGAGAG
  AGATCGTTGGATGGTCAAATAAAGATGGTGATGGGATATTTTCTCCTGGGGGAGCCATATCCAATATGTA
  CAGCATCATGGCTGCTCGTTACAAGTACTTCCCAGAAGTGAAGACAAAAGGCATGGCGGCTGTGCCCAAA
  CTGGTCCTCTTCACCTCAGAACACAGTCACTATTCCATAAAGAAAGCCGGGGCTGCGCTTGGCTTTGGAA
  CCGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAGATAATTCCGGCTGATTTAGAGGCAAAAAT
  TCTTGATGCCAAACAAAAGGGCTATGTTCCCCTTTATGTCAATGCAACCGCAGGCACGACTGTTTACGGA
  GCATTCGATCCAATCCAGGAAATTGCGGACATATGTGAGAAATACAACCTTTGGCTGCATGTGGATGCTG
  CCTGGGGTGGTGGACTGCTCATGTCCCGGAAGCACCGCCACAAACTCAGCGGCATAGAAAGGGCCAATTC
  AGTCACCTGGAACCCTCACAAGATGATGGGCGTGCTGCTCCAGTGCTCTGCCATTCTGGTCAAGGAAAAG
  GGTATACTCCAAGGATGCAACCAGATGTGTGCAGGCTACCTCTTCCAGCCAGACAAGCAGTATGACGTCT
  CCTATGACACCGGGGACAAGGCGATTCAGTGTGGCCGCCATGTGGACATCTTCAAGTTCTGGCTGATGTG
  GAAAGCAAAGGGCACCGTGGGATTTGAAAACCAGATCAACAAATGCCTGGAGCTGGCTGATTACCTCTAC
  GCCAAGATTAAAAACAGAGAAGAGTTTGAGATGGTTTTCGATGGTGAGCCTGAGCACACAAATGTCTGTT
  TCTGGTACATTCCACAAAGCCTTCGAGGGGTTCCAGATAGCCCTGAGCGACGAGAAAAGCTACACAGGGT
  GGCTCCCAAGATCAAAGCTCTGATGATGGAGTCAGGAACAACCATGGTGGGCTACCAGCCTCAAGGGGAC
  AAGGCCAACTTCTTCCGGATGGTCATCTCTAACCCAGCCGCCACCCAGTCTGACATCGATTTCCTCATTG
  AGGAGATAGAGAGGTTGGGTCAGGATCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209243 representing NM_008077
  Red=Cloning site Green=Tags(s)
  
  MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFRERQ
  SSKNLLSCENSDQGARFRRTETDFSNLFAQDLLPAKNGEEQTAQFLLEVVDILLNYVRKTFDRSTKVLDF
  HHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANT
  NMFTYEIAPVFVLMEQITLKKMREIVGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPK
  LVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKGYVPLYVNATAGTTVYG
  AFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEK
  GILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELADYLY
  AKIKNREEFEMVFDGEPEHTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGD
  KANFFRMVISNPAATQSDIDFLIEEIERLGQDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008077
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008077.5
• RefSeq Size: 3231 bp
• RefSeq ORF: 1782 bp
• Locus ID: 14415
• UniProt ID: P48318
• Cytogenetics: 2 41.63 cM
• Gene Summary: The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in two products, the predominant 67 kDa isoform and a smaller 25 kDa isoform. The smaller isoform is not thought to retain catalytic activity. [provided by RefSeq, Aug 2015]