Syt3 (NM_016663) Mouse Tagged ORF Clone

CAT#: MG209162

Syt3 (tGFP-tagged) - Mouse synaptotagmin III (Syt3)

Product Images

Circular map for MG209162

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Syt3
• Synonyms: AI385753; sytIII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209162 representing NM_016663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGGACTATGAAGATGACCTCTGTCGGCGGGCGCTCATCCTGGTCTCAGACCTCTGTGCACGGG
  TCCGAGATGCTGATACCAATGATAGGTGTCAAGAGTTCAACGAACTGCGGATCAGAGGATATCCCCGGGG
  TCCGGATGCAGATATTTCCGTGAGCCTGCTGTCAGTCATCGTGACATTCTGTGGCATCGTCCTCTTGGGT
  GTCTCCCTCTTCGTGTCCTGGAAGTTGTGCTGGGTGCCCTGGAGGGACAAAGGAGGCTCAGCAGTGGGTG
  GTGGCCCCCTGCGCAAAGACTTAGCCCCTGGGGTTGGGCTGGCAGGCCTGGTAGGAGGTGGTGGGCACCA
  CCTAGGAGCCAGCCTCGGTGGTCATCCCCTGCTGGGTGGCCCACACCATCATGGGCACACAGCCCACCAT
  CCACCATTTGCGGAGCTGCTGGAACCAGGTGGTCTTGGGGGCTCTGAGCCGCCAGAACCTTCCTACCTGG
  ACATGGACTCATATCCAGAGGCTGCTGTGGCCTCTGTGGTAGCTGCTGGAGTCAAACCAAGCCAGACATC
  CCCTGAGCTGCCTTCTGAGGGTGGAACAGGTTCTGGGCTGCTCCTGCTGCCTCCCAGTGGTGGGGGCTTA
  CCCAGTGCCCAGTCTCATCAGCAGGTCACAAGTCTAGCACCCACCACAAGGTACCCAGCCCTGCCTCGGC
  CCCTCACCCAGCAGACCCTGACCACCCAAGCAGACCCAAGCACTGAGGAGCGACCGCCTGCACTGCCCTT
  ACCTCTGCCGGGTGGAGAGGAAAAAGCCAAGCTCATCGGTCAGATCAAACCAGAGCTGTACCAGGGCACT
  GGACCCGGTGGTCGGCGAGGCGGGGGCTCAGGGGAAGCAGGGGCACCCTGTGGCCGCATCAGTTTTGCCC
  TCCGGTACCTCTATGGTTCTGACCATCTCGTGGTGCGGATCCTACAGGCCCTGGATCTCCCAGCAAAGGA
  CTCCAATGGGTTCTCAGACCCCTACGTCAAGATCTACCTGTTGCCTGATCGCAAAAAGAAGTTCCAGACC
  AAGGTGCACAGGAAGACGTTGAACCCCATCTTCAACGAGACGTTTCAGTTCTCGGTGCCCCTGGCCGAGC
  TGGCACAGCGCAAGCTGCACTTCAGCGTCTATGACTTCGACCGCTTCTCGCGGCATGATCTCATTGGCCA
  GGTGGTTTTGGACAACCTGCTGGAGCTGGCTGAACAGCCCCCTGACCGCCCGCTCTGGAGGGACATCCTG
  GAGGGTGGCTCGGAAAAGGCAGATCTCGGAGAGCTCAACTTCTCTCTCTGCTACCTCCCCACGGCTGGGC
  GCCTCACCGTGACCATCATCAAAGCCTCTAACCTCAAAGCAATGGACCTCACTGGTTTCTCAGACCCCTA
  TGTGAAGGCCTCTCTGATCAGCGAGGGGCGGCGTCTGAAGAAACGGAAGACCTCCATTAAGAAGAACACA
  CTGAACCCCACGTACAACGAGGCCCTGGTGTTCGACGTGGCTCCCGAGAGCGTGGAGAACGTGGGTCTCA
  GCATCGCAGTGGTAGACTATGACTGCATCGGCCACAATGAAGTGATCGGGGTGTGCCGCGTGGGTCCAGA
  GGCTGCCGACCCACACGGCAGAGAACACTGGGCAGAGATGCTGGCTAACCCGCGCAAGCCTGTGGAACAC
  TGGCACCAGCTAGTGGAGGAGAAGACTCTGAGCAGCTTCACAAAGGGTGGCAAAGGATTGTCAGAGAAAG
  AGAATTCAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209162 representing NM_016663
  Red=Cloning site Green=Tags(s)
  
  MSGDYEDDLCRRALILVSDLCARVRDADTNDRCQEFNELRIRGYPRGPDADISVSLLSVIVTFCGIVLLG
  VSLFVSWKLCWVPWRDKGGSAVGGGPLRKDLAPGVGLAGLVGGGGHHLGASLGGHPLLGGPHHHGHTAHH
  PPFAELLEPGGLGGSEPPEPSYLDMDSYPEAAVASVVAAGVKPSQTSPELPSEGGTGSGLLLLPPSGGGL
  PSAQSHQQVTSLAPTTRYPALPRPLTQQTLTTQADPSTEERPPALPLPLPGGEEKAKLIGQIKPELYQGT
  GPGGRRGGGSGEAGAPCGRISFALRYLYGSDHLVVRILQALDLPAKDSNGFSDPYVKIYLLPDRKKKFQT
  KVHRKTLNPIFNETFQFSVPLAELAQRKLHFSVYDFDRFSRHDLIGQVVLDNLLELAEQPPDRPLWRDIL
  EGGSEKADLGELNFSLCYLPTAGRLTVTIIKASNLKAMDLTGFSDPYVKASLISEGRRLKKRKTSIKKNT
  LNPTYNEALVFDVAPESVENVGLSIAVVDYDCIGHNEVIGVCRVGPEAADPHGREHWAEMLANPRKPVEH
  WHQLVEEKTLSSFTKGGKGLSEKENSE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016663
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016663.2, NP_057872.2
• RefSeq Size: 2432 bp
• RefSeq ORF: 1764 bp
• Locus ID: 20981
• UniProt ID: O35681
• Cytogenetics: 7 28.83 cM
• Gene Summary: Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. Plays a role in dendrite formation by melanocytes.[UniProtKB/Swiss-Prot Function]