Coil (BC031167) Mouse Tagged ORF Clone

CAT#: MG208968

Coil (tGFP-tagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919)

Product Images

Circular map for MG208968

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Coil
• Synonyms: Cln80, p80-coilin, MGC37472
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208968 representing BC031167
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCGAGACGGTTAGGCTACGGCTTCAATTTGACTACCCGCCGCCGGCCACTCCACACTGCA
  CGGTCTTCTGGCTTCTGGTGGACCTGAACAGATGTCGAGTTGTCACGGATCTCATCAGTCTCATCCGCCA
  GCGCTTCGGCTTCAGTTCCGGGGCGCTCCTGGGCCTCTATCTGGAAGGGGGGCTGCTGCCCCCTGCCGAG
  AGCGCGCGCCTGGTGAGAGACAACGACAGCCTCAGGGTTAAGTTAGAAGACCAAGGACTCCCTGAGAACC
  TGATAGTAAGCAATGGTGGCGACTCTAGTTTCCCATGCAGGAAAGCAAAGAAACGGGCTTTTAAGTTGAT
  GGAGGATGAGGAGACAGATCAGGGATACAAGTCTTCGAAGAAGCACTGCAAGAGGCAGGAGGACAGCGGC
  CAGAATGAGAAGGCCTCGGACCTGGAAACTAAACTCCTCCCAGATGAGACGGGGCGGACAAAGAGCAAGA
  AAAAGAGCAAAGTGACAGGCAGCCCAGCCGAAGAGGACGAGGAAGAGACCAAAAAGAAGTCTTCAAAAAG
  AAAGGAGAAGTGTGAACCGAAGAAGCAGACCAGGGCCTCCAAGTCTTCCAAACAACAGACGCCTAAAGAG
  GGGGCCTCACAGAACTGCAGCTTCCCCAGGGCCTCCCCCAGAAGCCTCGGTAAAGCCCGGAGGAGAAGCA
  GCACGGGGCTAAAAGGTAGCTCAGGCCACCTGTCAGAGTCTGAGTCCTGCCCAGAGTCAGTCAGCGATGG
  CCACTGCAGTGTCATGATGCAGGAGGTCACCTTTTCAGAGAAACTGTCAGCTGAGCTGTTGAAAGATGCA
  CCTGCTACTAAGACTGCAGCTGCCAACAGGCAGGCCTCAAAGCCTGGCTTTACCTTCAGCTCCGGCAAGG
  GCAAGGCCTCCAGAACCTCGTCATCTAGCTCAGACTCCAGTTCAGAGTCGGAAGACCAGTTCCTGGTGTC
  CAAGAACATGCTCGAGGGCGCATCTGGCTTCTTAAAGCCAACTGGCCTCTTTGCAGGGCAAGGGGGTTCT
  GGTCCAGGGCTGTCATTGGAGACTCCGGGTATTATGGGATGGAAGTCTTCTGACTCAAACCGGGGCAGAC
  AAGCTCCTGGTCCTCCCAGTACGCCTGTCCCCACCAGTTTGGGAAGAGGATGGGGGCGAGGAGAGGACTT
  GTTGTTTGGGAAGGGACTGAGGGGCCGGGGTGTTCGGGGCCGAGGGCGAGGGCGCGGGCAAGCGGTCTCC
  TGTGTCTTTAATAGAAGCTCTGAGAGTCAGAAGCAGAGACAGTTAAACGATATTCTAACAAACTCATCTG
  TGGTGATCCAGAATCCAGTAGAGCCCCCCAAGAAGGACTATAGTCTATTACCACTGTTGGCAGCTGCCCC
  TCAAGTCGGAGAAAAGATTGCATTTAAGCTTTTGGAACTCACATCCGACTACTCTCCTGATGTCTCTGAC
  TACAAGGAAGGAAAAATATTAAGTCACGATCCAGAGACCCAGCAAGTGGACATAGAGGTCCTCTCCTCGC
  TACCGGCTTTGAAAGAACCTGGGAAGTTTGATTTGGTCTATCACAATGAAAACGGAACCGAGGTGGTGGA
  ATACGCTGTGACTCAGGAGAAGAGGATCACCGTGCTCTGGAGAGAGTTGATCGATCCGAGGCTGATCATT
  GACTCTTCAGGTAGCATCTCGAGTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208968 representing BC031167
  Red=Cloning site Green=Tags(s)
  
  MAASETVRLRLQFDYPPPATPHCTVFWLLVDLNRCRVVTDLISLIRQRFGFSSGALLGLYLEGGLLPPAE
  SARLVRDNDSLRVKLEDQGLPENLIVSNGGDSSFPCRKAKKRAFKLMEDEETDQGYKSSKKHCKRQEDSG
  QNEKASDLETKLLPDETGRTKSKKKSKVTGSPAEEDEEETKKKSSKRKEKCEPKKQTRASKSSKQQTPKE
  GASQNCSFPRASPRSLGKARRRSSTGLKGSSGHLSESESCPESVSDGHCSVMMQEVTFSEKLSAELLKDA
  PATKTAAANRQASKPGFTFSSGKGKASRTSSSSSDSSSESEDQFLVSKNMLEGASGFLKPTGLFAGQGGS
  GPGLSLETPGIMGWKSSDSNRGRQAPGPPSTPVPTSLGRGWGRGEDLLFGKGLRGRGVRGRGRGRGQAVS
  CVFNRSSESQKQRQLNDILTNSSVVIQNPVEPPKKDYSLLPLLAAAPQVGEKIAFKLLELTSDYSPDVSD
  YKEGKILSHDPETQQVDIEVLSSLPALKEPGKFDLVYHNENGTEVVEYAVTQEKRITVLWRELIDPRLII
  DSSGSISST
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031167
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031167.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 1709 bp
• Locus ID: 12812
• Cytogenetics: 11 54.34 cM