Relb (NM_009046) Mouse Tagged ORF Clone

CAT#: MG208842

Relb (tGFP-tagged) - Mouse avian reticuloendotheliosis viral (v-rel) oncogene related B (Relb)

Product Images

Circular map for MG208842

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Relb
• Synonyms: shep
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208842 representing NM_009046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGTCGCCGCGCTGCCAGAGAGTCCGCGCCCGAGCTAGGGGCCTTGGGTTCCAGTGACCTCTCTT
  CCCTGTCACTAACGGTCTCCAGGACCACAGATGAATTGGAAATCATCGACGAATACATTAAGGAGAACGG
  CTTTGGCCTGGACGGGACACAGCTGAGTGAGATGCCGCGCCTGGTGCCCCGCGGGCCCGCCTCACTGAGC
  AGCGTCACGCTGGGCCCTGCTGCACCACCGCCTCCGGCCACGCCGTCCTGGAGCTGCACACTGGGCAGGC
  TGGTGTCACCCGGCCCGTGCCCACGGCCGTACCTGGTCATCACAGAGCAGCCAAAGCAGCGTGGCATGCG
  CTTCCGCTACGAGTGCGAGGGCCGCTCGGCCGGCAGCATCCTCGGGGAGAGCAGCACCGAAGCCAGCAAG
  ACCCTGCCCGCCATCGAGCTTCGAGACTGTGGCGGGCTGCGGGAGGTGGAGGTGACGGCGTGCCTGGTGT
  GGAAGGACTGGCCACACCGGGTACACCCACATAGCCTCGTGGGGAAAGACTGCACGGACGGCGTCTGCAG
  GGTGCGGCTGCGGCCTCACGTCAGCCCCCGGCACAGCTTTAACAACCTGGGCATCCAGTGTGTTAGGAAG
  AAGGAAATTGAAGCTGCCATTGAGCGGAAGATCCAGCTGGGAATTGACCCCTACAATGCTGGCTCCCTGA
  AGAACCATCAGGAGGTCGACATGAATGTCGTCAGGATCTGCTTCCAGGCCTCCTATCGGGACCAGCAGGG
  ACATCTGCACCGCATGGACCCCATCCTCTCTGAGCCTGTCTACGACAAGAAGTCCACCAACACATCGGAG
  CTGCGGATTTGCCGAATCAACAAGGAGAGCGGGCCGTGCACAGGTGGTGAGGAGCTGTACTTGCTCTGTG
  ACAAGGTGCAAAAAGAGGACATATCCGTGGTGTTCAGCACAGCTTCCTGGGAAGGCCGTGCCGACTTCTC
  TCAAGCTGATGTGCACCGGCAGATCGCCATTGTGTTCAAAACGCCACCCTACGAGGACCTGGAGATCTCA
  GAGCCCGTGACTGTCAATGTGTTCTTGCAGCGGCTCACGGATGGGGTGTGCAGCGAGCCGCTGCCCTTCA
  CGTACCTGCCTCGGGATCATGACAGCTACGGTGTGGACAAGAAGCGAAAGCGGGGACTGCCTGATGTCCT
  TGGAGAGTTGAGCAGCTCTGATCCACATGGAATCGAGAGCAAACGAAGGAAAAAGAAACCAGTGTTCTTG
  GACCACTTCCTGCCTGGCCACAGCTCAGGCCTGTTCCTCCCACCATCGGCTCTGCAGCCGGCAGACTCTG
  ATTTCTTCCCTGCTTCCATATCCCTTCCTGGGCTGGAGCCTCCTGGTGGACCCGATCTCCTGGACGATGG
  CTTTGCCTATGATCCTTCTGCCCCCACGCTCTTCACTATGTTGGACCTGCTGCCCCCAGCACCACCACTT
  GCCAGTGCTGTGGTGGGTAGCGGGGGTGCAGGGGCCACCGTTGTGGAGTCTTCTGGCCCAGAGCCCCTAT
  CACTGGACTCTTTTGCAGCGCCGGGCCCCGGGGATGTTGGTACTGCTAGCCTTGTGGGCAGCAACATGTT
  TCCCAACCAGTACCGAGAGGCAGCTTTCGGGGGTGGCCTCCTATCTCCAGGGCCTGAAGCCACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208842 representing NM_009046
  Red=Cloning site Green=Tags(s)
  
  MPSRRAARESAPELGALGSSDLSSLSLTVSRTTDELEIIDEYIKENGFGLDGTQLSEMPRLVPRGPASLS
  SVTLGPAAPPPPATPSWSCTLGRLVSPGPCPRPYLVITEQPKQRGMRFRYECEGRSAGSILGESSTEASK
  TLPAIELRDCGGLREVEVTACLVWKDWPHRVHPHSLVGKDCTDGVCRVRLRPHVSPRHSFNNLGIQCVRK
  KEIEAAIERKIQLGIDPYNAGSLKNHQEVDMNVVRICFQASYRDQQGHLHRMDPILSEPVYDKKSTNTSE
  LRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSTASWEGRADFSQADVHRQIAIVFKTPPYEDLEIS
  EPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGLPDVLGELSSSDPHGIESKRRKKKPVFL
  DHFLPGHSSGLFLPPSALQPADSDFFPASISLPGLEPPGGPDLLDDGFAYDPSAPTLFTMLDLLPPAPPL
  ASAVVGSGGAGATVVESSGPEPLSLDSFAAPGPGDVGTASLVGSNMFPNQYREAAFGGGLLSPGPEAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009046
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009046.2, NP_033072.2
• RefSeq Size: 2218 bp
• RefSeq ORF: 1677 bp
• Locus ID: 19698
• UniProt ID: Q04863
• Cytogenetics: 7 9.93 cM
• Gene Summary: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49 (By similarity). As a member of the NUPR1/RELB/IER3 survival pathway, may allow the development of pancreatic intraepithelial neoplasias. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (By similarity).[UniProtKB/Swiss-Prot Function]