Cdc25b (BC057568) Mouse Tagged ORF Clone

CAT#: MG208736

Cdc25b (GFP-tagged) - Mouse cell division cycle 25 homolog B (S. pombe) (cDNA clone MGC:66900 IMAGE:6401489)

Specifications

Product Data

• Product Name: Cdc25b (BC057568) Mouse Tagged ORF Clone
• Symbol: Cdc25b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208736 representing BC057568
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTACCCCTGCAGAAGTCTGCGCCGGGTTCAGCTCTCAGTCCTGCCCGCGTGCTGGGTGGCATTC
  AGCGGCCGCGCCACCTCTCGGTCTTTGAGTTTGAGTCTGATGGCTTCCTGGGGTCTCCGGAGCCTACAGC
  TTCCTCCTCTCCGGTTACCACTCTTACACAGACCATGCACAACCTCGCTGGGCTCGGCAGTGAGCCTCCA
  AAAGCTCAAGTAGGAAGCCTGTCGTTCCAGAACAGGCTGGCAGACCTATCCCTGTCCAGGCGCACCTCTG
  AGTGCTCCCTGTCATCTGAGTCCTCAGAATCTTCGGATGCAGGTCTGTGCATGGACTCCCCCAGCCCTGT
  GGACCCGCAGATGGCAGAGCGCACGTTTGAACAGGCCATTCAGGCAGCCAGTCGGGTCATTCAAAATGAG
  CAGTTTACCATAAAACGCTTCCGATCCTTACCAGTGAGGCTGCTGGAACACAGTCCGGTGCTGCAGAGCA
  TCACCAACTCCCGAGCACTGGACAGCTGGAGGAAAACTGAAGCAGGCTACCGAGCCGCCGCCAATAGTCC
  TGGGGAGGACAAAGAGAATGTGCGCTTCCAGAGACCAAGATGGAGGAGCTCCAGGGAGAGAAGGTGTCTT
  ACCACTGAGTGGAAGATGGAAGTAGAGGAGCTGAGCCCGGTGGCACAGTCTTCTTCCTTGACTCCTGTCG
  AAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACATCCTGGAGAGTGATTTAAAGGATGACGAGAAGGT
  CCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACTGGTCAAAAAGCTGGATAAGGAAGAGGAACAGGAT
  CTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTGATCCGACCCA
  TCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGAAAAGTGTGAC
  ACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGCCCGTGTCTTTCGCTCAAAGTCGCTGTGTCATGAG
  ATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAGGCCTTCCTCCTGCAGA
  CCGTGGATGGCAAACACCAAGACCTTAAGTACATCTCACCAGAAACTATGGTGGCCCTGTTAACAGGCAA
  GTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGACTGCAGATACCCCTATGAGTATGAAGGCGGGCAT
  ATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGATGCTGAGACCTTTCTGCTGCAGCGTCCCATCATGC
  CTTGTAGCCTGGACAAGAGAATCATCCTCATTTTCCACTGTGAATTCTCGTCTGAGCGTGGACCACGAAT
  GTGCCGCTTCATCAGGGAACGGGACCGTGCAGCTAACGACTACCCCAGCCTGTACTACCCGGAGATGTAC
  ATCCTCAAAGGCGGCTACAAGGAGTTCTTCCCACAGCATCCGAACTTTTGTGAGCCCCAGGACTACCGAC
  CCATGAACCACGAGGCTTTCAGGGATGAGCTGAGGAACTTTCGCCTTAAGACTCGCAGCTGGGCTGGGGA
  ACGGAGCAGGAGGGAACTTTGTAGCAGGCTGCAAGACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208736 representing BC057568
  Red=Cloning site Green=Tags(s)
  
  MEVPLQKSAPGSALSPARVLGGIQRPRHLSVFEFESDGFLGSPEPTASSSPVTTLTQTMHNLAGLGSEPP
  KAQVGSLSFQNRLADLSLSRRTSECSLSSESSESSDAGLCMDSPSPVDPQMAERTFEQAIQAASRVIQNE
  QFTIKRFRSLPVRLLEHSPVLQSITNSRALDSWRKTEAGYRAAANSPGEDKENVRFQRPRWRSSRERRCL
  TTEWKMEVEELSPVAQSSSLTPVERASEEDDGFVDILESDLKDDEKVPAGMENLISAPLVKKLDKEEEQD
  LIMFSKCQRLFRSPSMPCSVIRPILKRLERPQDRDVPVQSKRRKSVTPLEEQQLEEPKARVFRSKSLCHE
  IENILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGH
  IKNAVNLPLERDAETFLLQRPIMPCSLDKRIILIFHCEFSSERGPRMCRFIRERDRAANDYPSLYYPEMY
  ILKGGYKEFFPQHPNFCEPQDYRPMNHEAFRDELRNFRLKTRSWAGERSRRELCSRLQDQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: TurboGFP
• ACCN: BC057568
• ORF Size: 1652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: BC057568
• RefSeq Size: 2935
• RefSeq ORF: 1652
• Locus ID: 12531
• Cytogenetics: 2 63.29 cM