Eif3d (NM_018749) Mouse Tagged ORF Clone

CAT#: MG208705

Eif3d (tGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit 7 (zeta) (Eif3s7)

Product Images

Circular map for MG208705

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eif3d
• Synonyms: 66/67kDa; AA407891; eIF3p66; Eif3s7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208705 representing NM_018749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGTTCATGACACCTGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCCGTTCCTGAGC
  AATTTCGGGATATGCCCTACCAGCCATTCAGCAAAGGAGATCGGCTGGGAAAGGTTGCAGACTGGACAGG
  GGCCACATACCAGGACAAGAGGTACACAAACAAGTATTCCTCTCAGTTCGGTGGGGGAAGTCAGTATGCA
  TATTTCCATGAGGAGGATGAGACAAGCTTCCAGCTGGTGGACACGGCACGGACACAGAAGACCGCCTACC
  AGCGGAACCGGATGAGATTCGCACAGCGCAACCTCCGCAGAGACAAAGATCGGAGGAACATGGTGCAGTT
  CAACCTACAGACCCTGCCCAAGAGTGCCAAGCAGAAAGAGAGAGAACGAATTCGCTTGCAGAAAAAATTC
  CAGAAGCAATTTGGAGTGAGGCAAAAATGGGACCAGAAGTCACAGAAACCCCGAGACTCCTCAGTTGAAG
  TTCGCAGTGACTGGGAGGTGAAGGAGGAGATGGACTTCCCTCAGCTGATGAAGATGCGCTACTTGGAAGT
  GTCAGAGCCTCAAGACATCGAGTGCTGCGGAGCCCTGGAGTACTACGACAAAGCCTTTGACCGCATCACC
  ACAAGGAGTGAGAAGCCCCTGCGGAGCATCAAGCGCATCTTTCACACCGTCACCACCACAGACGACCCTG
  TCATCCGGAAGCTGGCAAAAACGCAGGGCAATGTGTTTGCCACTGACGCCATCCTGGCCACGCTGATGAG
  CTGCACCCGCTCCGTGTACTCCTGGGACATCGTTGTCCAGAGAGTCGGCTCTAAACTCTTCTTTGACAAG
  AGGGACAACTCTGACTTTGACCTCCTGACTGTGAGTGAGACAGCCAATGAGCCGCCTCAAGATGAAGGCA
  ACTCCTTCAACTCACCCCGGAACCTGGCCATGGAAGCCACCTACATCAACCACAACTTCTCTCAGCAGTG
  CCTGAGAATGGGAAGAGAAAGATACAATTTCCCCAACCCAAACCCATTTGTGGAGGACGACATGGATAAG
  AATGAGATCGCCTCCGTCGCTTACCGTTACCGCAGGTGGAAGCTTGGAGATGACATCGACCTTATCGTCC
  GCTGTGAACACGATGGTGTCATGACCGGGGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACGCTCAA
  TGAATGGGACTCCAGGCATTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGTGGGGCTGTC
  ATCGCCACTGAATTGAAGAACAACAGCTACAAGTTGGCACGGTGGACCTGCTGTGCTTTGCTGGCTGGAT
  CTGAGTACCTGAAGCTCGGGTATGTGTCCCGGTACCACGTAAAGGACTCCTCACGCCATGTCATCCTGGG
  CACCCAGCAGTTTAAGCCCAATGAGTTTGCCAGTCAGATCAACCTGAGCGTGGAGAATGCCTGGGGCATC
  CTGCGCTGCGTCATCGACATCTGCATGAAGCTGGAGGAGGGCAAGTACCTCATCCTCAAGGACCCCAACA
  AGCAGGTCATCCGGGTCTACAGCCTGCCTGATGGCACCTTCAGCTCTGAGGAGGACGAAGAGGACGAGGA
  GGAGGAAGAGGAGGAAGAGGAAGAGGAAGAAACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208705 representing NM_018749
  Red=Cloning site Green=Tags(s)
  
  MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYA
  YFHEEDETSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMVQFNLQTLPKSAKQKERERIRLQKKF
  QKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRIT
  TRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDK
  RDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGRERYNFPNPNPFVEDDMDK
  NEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAV
  IATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGI
  LRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSEEDEEDEEEEEEEEEEEET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018749
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018749.2, NP_061219.2
• RefSeq Size: 1910 bp
• RefSeq ORF: 1647 bp
• Locus ID: 55944
• UniProt ID: O70194
• Cytogenetics: 15 E1
• Gene Summary: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.[UniProtKB/Swiss-Prot Function]