Prmt3 (BC050775) Mouse Tagged ORF Clone

CAT#: MG208535

Prmt3 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 3 (cDNA clone MGC:58346 IMAGE:6534023)

Product Images

Circular map for MG208535

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prmt3
• Synonyms: 2010005E20Rik; 2410018A17Rik; AL033309; Hrmt1l3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208535 representing BC050775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTTCGCTAGCGGCGGGGAACGGCCGTGGCGCCGAGCTGGGGCCGGAGCCCCTGGAACTGTCGGACA
  GCGGAGACGACGCGGGCTGGGAGGACGAGGACGCGGACACTGAGCCCGCGCACGGCCGGCAGCACACACC
  CTGCCTGTTCTGTGACAGGTTGTTCGCCTCTGCTGAGGAAACGTTTTCACACTGTAAGTTGGAGCATCAA
  TTTAATATTGATAGTATGGTCCATAAACATGGACTTGAATTTTATGGATACATTAAACTAATAAATTTTA
  TTAGACTTAAGAATCCTACGGTTGAATATATGAATTCCATATATAACCCTGTGCCTTGGGAAAAAGATGA
  GTACCTGAAGCCAGTGTTGGAAGATGACCTTTTGCTTCAGTTTGATGTAGAAGATCTTTATGAGCCTGTG
  TCTACTCCATTCTCATACCCCAATGGACTCAGTGAAAGCGCATCTGTTGTTGAGAAGTTGAAGCACATGG
  AAGCGCGGGCGCTGTCTGCTGAGGCTGCCTTGGCTCGGGCGCGTGAGGATCTGCAGAAGATGAAACAATT
  TGCTCAGGACTTTGTGATGAACGTAGATGTCAGAACCTGCTCCTCCACGACGACCATTGCAGACCTGCAG
  GAGGATGAGGATGGCGTCTACTTCAGCTCCTATGGGCATTATGGGATACATGAAGAAATGCTAAAGGACA
  AAGTACGCACAGAAAGTTACAGAGATTTCATATACCAAAATCCACACATCTTCAAAGATAAGGTTGTTCT
  GGATGTTGGGTGTGGAACTGGAATTCTCTCCATGTTTGCTGCCAAAGTTGGGGCAAAGAAAGTGATTGCA
  GTCGACCAGTCTGAAATCCTCTACCAGGCTATGGATATCATAAGACTAAATAAACTTGAAGATACCATTG
  TACTAATTAAAGGAAAGATTGAAGAAGTGAGTCTTCCTGTGGAAAAGGTGGATGTTATTATATCAGAGTG
  GATGGGCTATTTTCTTCTTTTTGAGTCGATGTTGGATTCTGTCCTTTATGCTAAGAGTAAATACTTGGCA
  AAAGGGGGATCGGTCTACCCTGACATTTGCACCATCAGCCTTGTAGCTGTGAGTGATGTGAGCAAGCACG
  CCGATAGAATTGCCTTCTGGGATGACGTCTATGGCTTCAACATGTCCTGCATGAAGAAAGCAGTTATACC
  GGAAGCTGTTGTGGAAGTTGTGGATCACAAGACTCTTATTTCAGACCCTTGTGATATCAAGATGGATGGG
  AAGCACATAGACTGCCACACCACGTCTATCTCAGACTTGGAGTTTTCTTCAGATTTTACCCTGAGAACCA
  CAAAGACGGCCATGTGCACGGCAGTTGCTGGGTACTTTGATATATATTTTGAGAAGAATTGCCACAACAG
  GGTCGTGTTCTCTACTGGCCCCCAGAGCACCAAAACACACTGGAAACAGACAGTATTTCTGCTGGAAAAA
  CCTTTTCCGGTTAAAGCAGGTGAAGCTCTGAAAGGAAAGATCACAGTTCACAAGAATAAGAAGGATCCTC
  GTTCCCTCATTGTGACCCTGACTTTGAACAGTTCAACCCAGACTTACAGTCTCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208535 representing BC050775
  Red=Cloning site Green=Tags(s)
  
  MCSLAAGNGRGAELGPEPLELSDSGDDAGWEDEDADTEPAHGRQHTPCLFCDRLFASAEETFSHCKLEHQ
  FNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKDEYLKPVLEDDLLLQFDVEDLYEPV
  STPFSYPNGLSESASVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMNVDVRTCSSTTTIADLQ
  EDEDGVYFSSYGHYGIHEEMLKDKVRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKVGAKKVIA
  VDQSEILYQAMDIIRLNKLEDTIVLIKGKIEEVSLPVEKVDVIISEWMGYFLLFESMLDSVLYAKSKYLA
  KGGSVYPDICTISLVAVSDVSKHADRIAFWDDVYGFNMSCMKKAVIPEAVVEVVDHKTLISDPCDIKMDG
  KHIDCHTTSISDLEFSSDFTLRTTKTAMCTAVAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEK
  PFPVKAGEALKGKITVHKNKKDPRSLIVTLTLNSSTQTYSLQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050775
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050775.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1598 bp
• Locus ID: 71974
• Cytogenetics: 7 B4
• Gene Summary: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.[UniProtKB/Swiss-Prot Function]