P4hb (NM_011032) Mouse Tagged ORF Clone

CAT#: MG208187

P4hb (tGFP-tagged) - Mouse prolyl 4-hydroxylase, beta polypeptide (P4hb)

Product Images

Circular map for MG208187

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: P4hb
• Synonyms: ERp59; PDI; Pdia1; Thbp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208187 representing NM_011032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCCGTGCTTTGCTGTGCCTAGCCCTGGCCTGGGCGGCTAGGGTGGGCGCCGACGCCCTGGAGG
  AGGAGGACAACGTCCTGGTGTTGAAGAAGAGCAACTTCGAGGAGGCGCTGGCGGCGCACAAGTACCTGCT
  GGTGGAGTTCTATGCCCCGTGGTGTGGCCACTGCAAAGCTCTGGCTCCCGAGTATGCCAAAGCTGCCGCA
  AAACTGAAGGCAGAAGGCTCTGAGATTCGACTAGCAAAGGTGGATGCCACAGAAGAGTCTGACCTGGCTC
  AGCAGTATGGTGTCCGTGGCTACCCCACAATCAAGTTCTTCAAGAATGGAGACACAGCCTCCCCAAAGGA
  ATATACAGCTGGCAGGGAAGCTGATGACATTGTCAACTGGCTGAAGAAACGCACGGGCCCAGCAGCTACA
  ACCCTGTCTGACACTGCAGCTGCAGAGTCCTTGGTGGACTCAAGCGAAGTGACGGTCATCGGTTTCTTCA
  AGGACGTAGAGTCAGACTCTGCCAAGCAGTTCTTGCTGGCAGCAGAGGCTATTGATGACATACCTTTTGG
  AATCACGTCCAACAGTGGTGTGTTCTCCAAGTACCAGCTGGACAAAGATGGGGTGGTCCTCTTTAAGAAG
  TTTGATGAAGGCCGCAACAACTTTGAGGGTGAGATCACCAAGGAGAAGCTTCTAGACTTCATCAAGCACA
  ACCAGCTGCCTTTGGTCATCGAGTTCACTGAACAGACAGCTCCGAAGATTTTTGGAGGTGAGATCAAGAC
  ACACATTCTGCTGTTCCTGCCCAAGAGTGTATCTGACTATGACGGCAAGCTGAGCAGCTTCAAGAGAGCA
  GCCGAGGGCTTCAAGGGCAAGATCCTATTTATCTTCATCGATAGCGACCACACTGATAACCAGCGCATAC
  TTGAGTTCTTTGGCCTGAAGAAGGAGGAGTGTCCCGCCGTGCGGCTTATTACCCTAGAGGAAGAGATGAC
  CAAGTACAAACCAGAGTCAGATGAGCTGACGGCTGAGAAAATCACAGAGTTTTGCCACCGCTTCTTAGAG
  GGCAAGATCAAGCCCCACCTGATGAGCCAGGAAGTGCCTGAAGACTGGGACAAACAGCCGGTAAAAGTAC
  TGGTTGGGGCGAACTTTGAAGAGGTCGCTTTTGATGAGAAAAAGAACGTGTTTGTTGAATTCTATGCCCC
  TTGGTGTGGTCACTGCAAGCAGCTAGCCCCCATTTGGGATAAACTGGGAGAGACATACAAGGATCATGAG
  AATATCATCATTGCTAAGATGGACTCAACAGCCAATGAGGTGGAAGCTGTCAAAGTACATAGCTTTCCCA
  CACTGAAGTTCTTCCCAGCAAGTGCAGACAGAACGGTCATTGATTACAATGGCGAGCGGACACTGGACGG
  TTTTAAGAAATTCTTGGAGAGTGGTGGCCAGGATGGAGCAGGGGACGATGAGGACCTCGACCTAGAAGAA
  GCTTTAGAGCCAGACATGGAGGAAGACGACGATCAGAAAGCTGTGAAGGATGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208187 representing NM_011032
  Red=Cloning site Green=Tags(s)
  
  MLSRALLCLALAWAARVGADALEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAA
  KLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAAT
  TLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKDGVVLFKK
  FDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKRA
  AEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLE
  GKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHE
  NIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDEDLDLEE
  ALEPDMEEDDDQKAVKDEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011032
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011032.1
• RefSeq Size: 2538 bp
• RefSeq ORF: 1530 bp
• Locus ID: 18453
• UniProt ID: P09103
• Cytogenetics: 11 84.27 cM
• Gene Summary: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP (By similarity). Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).[UniProtKB/Swiss-Prot Function]