Fip1l1 (BC003263) Mouse Tagged ORF Clone

CAT#: MG208168

Fip1l1 (tGFP-tagged) - Mouse FIP1 like 1 (S. cerevisiae) (cDNA clone MGC:7777 IMAGE:3499130)

Product Images

Circular map for MG208168

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fip1l1
• Synonyms: 1300019H17Rik; Rje
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208168 representing BC003263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTGGAGCGCCTGGTGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGT
  GGCTGTATGGCGGCCCGTGGGACGTGCATGTGCACAGTGACTTGGCAAAGGATCTAGATGAGAATGAAGT
  TGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGAAGAAGCTGCTGAAAAT
  GGCGTGGCAAAACCGAAAGTGACAGAGACTGAAGACGACAGTGATAGTGACAGTGACGATGATGAAGATG
  ATGTTCACGTCACTATAGGAGACATCAAAACAGGAGCACCACAGTATGGGAGTTATGGAACAGCACCAGT
  AAATCTTAATATCAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTGGACCTC
  GATGCACCTGGCAGCATTAATGGAGTTCCACTCTTGGAAGTAGATCTGGATTCTTTTGAAGATAAACCAT
  GGCGGAAACCTGGTGCTGATCTTTCTGATTATTTTAACTATGGCTTTAATGAAGATACTTGGAAAGCTTA
  CTGTGAAAAACAAAAGAGGATACGAATGGGACTGGAAGTTATACCTGTTACTTCAACCACAAACAAGATT
  ACGGCCGAAGACTGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATCTTTTTA
  AGGTACAGCAAGGGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTAC
  TTCTCCGCCATCATTGTTCAAGACTGGTCTGCCACCAAGCAGAAACAGCACCTCTTCTCAGTCTCAGACA
  AGTACTGCCTCCAGAAAAGCCAGTTCAAGCGTTGGGAAGTGGCAGGATCGATATGGGAGGGCCGAGTCAC
  CTGATCTAAGGAGATTACCTGGAGCAATTGATGTCATTGGTCAAACAATAACCATCAGCCGAGTGGAAGG
  AAGACGGCGTGCAAATGAGAACAGCAATATACAGGTCCTTTCTGACAGATCTGCCACTGAAGTAGACAAC
  AATTTCAGCAAACCACCTCCATTTTTCCCTCCAGGGGCTCCTCCTACTCACCTTCCTCCTCCTCCATTTC
  TCCCACCACCTCCAACTGTCAGCACCGCCCCACCTCTCATTCCACCACCAGGTTTCCCCCCTCCACCAGG
  CGCCCCACCTCCATCTCTTATACCAACAATAGAAAGTGGCCATTCCTCCGGGTATGACAGCCGTTCTGCA
  CGAGCGTTTCCCTATGGCAATGTCGCCTTTCCCCACCTTACCAGTTCTGCTCCCTCATGGCCGAGCCTTG
  TGGACACCACCAAGCAGTGGGACTATTATGCAAGGAGAGAAAAAGACCGAGACCGAGACCGGGAGAGAGA
  CCGAGACAGAGAGCGAGAGCGGGACCGGGACAGAGAAAGGGAGCGCACCCGGGAGCGGGAGCGAGAGCGC
  GACCACAGCCCCACCCCAAGTGTTTTCAACAGGTTTGTTGGGTGTGCGGGGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208168 representing BC003263
  Red=Cloning site Green=Tags(s)
  
  MSAGEVERLVELSGGTGGDEEEEWLYGGPWDVHVHSDLAKDLDENEVERPEEENASANPPSGIEEEAAEN
  GVAKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKAGGRVYGNTGTKVKGVDL
  DAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKI
  TAEDCTMEVTPGAEIQDGRFNLFKVQQGRTGNSEKEAALPSTKAEFTSPPSLFKTGLPPSRNSTSSQSQT
  STASRKASSSVGKWQDRYGRAESPDLRRLPGAIDVIGQTITISRVEGRRRANENSNIQVLSDRSATEVDN
  NFSKPPPFFPPGAPPTHLPPPPFLPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRSA
  RAFPYGNVAFPHLTSSAPSWPSLVDTTKQWDYYARREKDRDRDRERDRDRERERDRDRERERTRERERER
  DHSPTPSVFNRFVGCAGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003263
• ORF Size: 1526 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003263, AAH03263
• RefSeq Size: 2109 bp
• RefSeq ORF: 1526 bp
• Locus ID: 66899
• Cytogenetics: 5 C3.3
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).[UniProtKB/Swiss-Prot Function]