Fip1l1 (BC003263) Mouse Untagged Clone

CAT#: MC217830

Fip1l1 (untagged) - Mouse FIP1 like 1 (S. cerevisiae) (cDNA clone MGC:7777 IMAGE:3499130), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fip1l1
• Synonyms: 1300019H17Rik; Rje
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTGGAGCGCCTGGTGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGT
  GGCTGTATGGCGGCCCGTGGGACGTGCATGTGCACAGTGACTTGGCAAAGGATCTAGATGAGAATGAAGT
  TGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGAAGAAGCTGCTGAAAAT
  GGCGTGGCAAAACCGAAAGTGACAGAGACTGAAGACGACAGTGATAGTGACAGTGACGATGATGAAGATG
  ATGTTCACGTCACTATAGGAGACATCAAAACAGGAGCACCACAGTATGGGAGTTATGGAACAGCACCAGT
  AAATCTTAATATCAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTGGACCTC
  GATGCACCTGGCAGCATTAATGGAGTTCCACTCTTGGAAGTAGATCTGGATTCTTTTGAAGATAAACCAT
  GGCGGAAACCTGGTGCTGATCTTTCTGATTATTTTAACTATGGCTTTAATGAAGATACTTGGAAAGCTTA
  CTGTGAAAAACAAAAGAGGATACGAATGGGACTGGAAGTTATACCTGTTACTTCAACCACAAACAAGATT
  ACGGCCGAAGACTGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATCTTTTTA
  AGGTACAGCAAGGGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTAC
  TTCTCCGCCATCATTGTTCAAGACTGGTCTGCCACCAAGCAGAAACAGCACCTCTTCTCAGTCTCAGACA
  AGTACTGCCTCCAGAAAAGCCAGTTCAAGCGTTGGGAAGTGGCAGGATCGATATGGGAGGGCCGAGTCAC
  CTGATCTAAGGAGATTACCTGGAGCAATTGATGTCATTGGTCAAACAATAACCATCAGCCGAGTGGAAGG
  AAGACGGCGTGCAAATGAGAACAGCAATATACAGGTCCTTTCTGACAGATCTGCCACTGAAGTAGACAAC
  AATTTCAGCAAACCACCTCCATTTTTCCCTCCAGGGGCTCCTCCTACTCACCTTCCTCCTCCTCCATTTC
  TCCCACCACCTCCAACTGTCAGCACCGCCCCACCTCTCATTCCACCACCAGGTTTCCCCCCTCCACCAGG
  CGCCCCACCTCCATCTCTTATACCAACAATAGAAAGTGGCCATTCCTCCGGGTATGACAGCCGTTCTGCA
  CGAGCGTTTCCCTATGGCAATGTCGCCTTTCCCCACCTTACCAGTTCTGCTCCCTCATGGCCGAGCCTTG
  TGGACACCACCAAGCAGTGGGACTATTATGCAAGGAGAGAAAAAGACCGAGACCGAGACCGGGAGAGAGA
  CCGAGACAGAGAGCGAGAGCGGGACCGGGACAGAGAAAGGGAGCGCACCCGGGAGCGGGAGCGAGAGCGC
  GACCACAGCCCCACCCCAAGTGTTTTCAACAGGTTTGTTGGGTGTGCGGGGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC003263
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003263, AAH03263
• RefSeq Size: 2109 bp
• RefSeq ORF: 1526 bp
• Locus ID: 66899
• Cytogenetics: 5 C3.3
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).[UniProtKB/Swiss-Prot Function]