Mboat1 (NM_153546) Mouse Tagged ORF Clone

CAT#: MG207891

Mboat1 (tGFP-tagged) - Mouse membrane bound O-acyltransferase domain containing 1 (Mboat1)

Product Images

Circular map for MG207891

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mboat1
• Synonyms: 9130215M02Rik; BC023845; LPEAT1; Moact1; Oact1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207891 representing NM_153546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCACGGCCGCCCGCCAGCCTCTCTTACCGTACCACCGGCTCCACCTGCCTGCACCCGCTCAGCC
  AGCTCCTGGGCATCCCGCTGGATCAGGTTAACTTTGTGGCTTGCCAGCTCTTTGCCTTGTCTGCTGCTTT
  CTGGTTCAGAATCTACTTACATCCTGGTAAAGCCAGCCCTGAGGTCCGGCACACCTTGGCCACCATTTTG
  GGCATCTATTTTGTTGTGTTTTGTTTTGGTTGGTATGCTGTACATCTCTTTGTGCTGGTGTTGATGTGTT
  ATGGGGTCATGGTCACTGCAAGTGTATCCAATATTCACAGGTATTCCTTTTTTGTAGCCATGGGCTACCT
  TACGATATGCCACATCAGCCGCATTTACATCTTCCACTATGGAATTCTCACTACAGATTTTTCTGGGCCC
  CTGATGATTGTCACTCAGAAGATCACGACGTTGGCTTTCCAAGTTCATGATGGATTGGGTCGAAAAGCTG
  AAGACCTTTCTGCTGAGCAACACCGACTTGCTGTGAAAGCGAAGCCCTCGCTTCTGGAATACTTAAGCTA
  CCATCTCAACTTTATGAGTGTCATAGCCGGCCCTTGCAACAATTTCAAGGACTACGTAGCCTTCATCGAA
  GGGAGACATATACACATGAAGTTGCTGGAAGTGAACTGGACGCAAAGGGGTTTCCAGAGTTTGCCAGAGC
  CTTCTCCCATGGGAGCTGTGATACAGAAGTTGTGTGTGACCTTGATGTCTCTCCTGTTGTTTTTGACGCT
  CTCCAAGTCCTTTCCCGTCACCTTCCTTATTGATGACTGGTTTGTACATAAGGCCAACTTTCTGAGTCGT
  CTCTGGTACTTATATGTCGTCATGCAAGCCGCAAAGCCCAAGTATTACTTTGCGTGGACATTAGCAGATG
  CGGTGCACAATGCAGCTGGATTCGGGTTCAATGGCATGGACACGGATGGGAAGTCTCGCTGGGATTTACT
  ATCTAACCTGAACATCTGGAAGATTGAGACTGCCACGAGTTTCAAAATGTACTTGGAAAACTGGAATATT
  CAGACATCTACGTGGCTGAAATGTGTGTGCTATGAGCGGGTTCCCTGGTACCCCACAGTGCTCACCTTCC
  TCCTGTCTGCTCTGTGGCACGGCGTCTACCCTGGATACTACTTCACATTCCTAACTGGAGTCCCTGTCAC
  ATTGGCAGCCAGAGCGGTGAGGAACAACTACAGACACCACTTCCTCTCTTCCAAAGCTCGAAAGATTGCC
  TATGACGTGGTGACCTGGGCTGTCACTCAGTTGGCTGTCTCTTACACGGCAGCGCCTTTCGTCATGTTGG
  CAGTCGAGCCAACCATCAGTTTATACAAGTCCGTGTTCTTTTTTTTACACATCATATGTCTGCTGATAAT
  CCTCTTTCTGCCAATCAAACCACACCAGCCTCAAAGGCAGTCTCGGAGTCCAAATTCTGTAAAGAAGAAG
  GCAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207891 representing NM_153546
  Red=Cloning site Green=Tags(s)
  
  MAARPPASLSYRTTGSTCLHPLSQLLGIPLDQVNFVACQLFALSAAFWFRIYLHPGKASPEVRHTLATIL
  GIYFVVFCFGWYAVHLFVLVLMCYGVMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGP
  LMIVTQKITTLAFQVHDGLGRKAEDLSAEQHRLAVKAKPSLLEYLSYHLNFMSVIAGPCNNFKDYVAFIE
  GRHIHMKLLEVNWTQRGFQSLPEPSPMGAVIQKLCVTLMSLLLFLTLSKSFPVTFLIDDWFVHKANFLSR
  LWYLYVVMQAAKPKYYFAWTLADAVHNAAGFGFNGMDTDGKSRWDLLSNLNIWKIETATSFKMYLENWNI
  QTSTWLKCVCYERVPWYPTVLTFLLSALWHGVYPGYYFTFLTGVPVTLAARAVRNNYRHHFLSSKARKIA
  YDVVTWAVTQLAVSYTAAPFVMLAVEPTISLYKSVFFFLHIICLLIILFLPIKPHQPQRQSRSPNSVKKK
  AD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153546
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153546.4
• RefSeq Size: 2900 bp
• RefSeq ORF: 1479 bp
• Locus ID: 218121
• UniProt ID: Q8BH98
• Cytogenetics: 13 A3.2
• Gene Summary: Acyltransferase which mediates the conversion of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity). Catalyzes also the acylation of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.[UniProtKB/Swiss-Prot Function]