Osbpl2 (NM_144500) Mouse Tagged ORF Clone

CAT#: MG207752

Osbpl2 (tGFP-tagged) - Mouse oxysterol binding protein-like 2 (Osbpl2)

Product Images

Circular map for MG207752

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Osbpl2
• Synonyms: C130070J12Rik; mKIAA0772; ORP-2; Orp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207752 representing NM_144500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGGGAGGAAGAATTCTTTGATGCCGTTACAGGCTTTGATTCTGATAATTCTTCTACAGGGGAAT
  TTTCAGAGGCAAATAAAATTAGTGGGATGATTGACTTGGATACCAGCAAAAGTACTAGGAGTGGGAAAAA
  TGGGGAGAAACCACAACAAGAGAATGGAATTCAAAAGCACAGGACAGCCCTTCCTGCTCCCATGTTCACC
  AGAAGCGACTTCAGTGTGTGGAGTATCCTGAAGAAATGCATTGGCTTGGAACTCTCCAAGATCACAATGC
  CAATTGCCTTCAATGAGCCACTGAGCTTCCTGCAGCGGATCACAGAGTACATGGAGCATGTGTACCTCAT
  TCACAAAGCCTCAAGTCAGTCCCAGCCGCTGGAGAGAATGCAGTCTGTCGCTGCCTTTGCGGTCTCAGCT
  GTGGCTTCCCAGTGGGAGAGAACTGGCAAACCCTTTAACCCACTCTTGGGAGAGACATATGAGTTGATCA
  GGGAAGACTTAGGGTTCAGATTTATATCTGAGCAGGTCAGTCACCACCCTCCTATTAGCGCATTTTATTC
  AGAAGGTCTCAATCAAGACTTCAGGTTCCACGGTTCCATCTACCCAAAGCTGAAGTTCTGGGGCAAGAGT
  GTGGAGGCGGAGCCCCGGGGGACCATCACACTGGAGCTGCTCAAACATAATGAAGCCTATACCTGGACCA
  ACCCTACCTGCTGTGTACACAATGTCATCCTTGGGCAGCTATGGATAGAGCAGTATGGGATAGTGGAGAT
  TGTAAATCACAGAACTGGAGATAAGTGTATACTTCACTTCAAGCCTTGTGGATTGTTTGGGAAGGAACTT
  CACAGAGTAGAAGGGTACATTCAAGACAAAAACAGGAAGAAGCTCTTTATCATGTATGGCAAGTGGACAG
  AGTGCTTGTGGGGAATAGATCCTGCTTCATACGAGTCCTTTAAGAAGCAAGAGAAGAGAGGTGACCAGGC
  CAGGAAGGCCAAGATGGATGATGGCCCCGAGAAAGCTAACAGTGATGTGCCTGGTGATGTGGCAGACGAC
  GTTCCTGTGGCTCAGGAGACGGTGCAAGTCATCCCCGGCAGCAAGCTGCTCTGGAGGATCAACAGCCGGC
  CCCCCAACTCTGCTCAGATGTACAACTTTACCAGCTTCACTGTGAGTCTCAATGAGTTGGAGTCAGGCAT
  GGAGAAGACTCTGCCACCCACAGACTGCCGCCTCCGTCCTGACATACGAGGCATGGAGAACGGAAACATG
  GATCTGGCCAGCCAGGAGAAGGAGCGGCTAGAGGAAAAGCAGAGAGAGGCCAGAAAAGAGCGTGCCAAAG
  AGGACGCAGAGTGGCGGACCAGGTGGTTCTCTCCAGGCAATAACCCCTACACAGGAGCTCCTGACTGGCT
  GTACGCCGGCCATTACTTTGAGCGTAACTTCTCAGACTGCCCAGACATATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207752 representing NM_144500
  Red=Cloning site Green=Tags(s)
  
  MNGEEEFFDAVTGFDSDNSSTGEFSEANKISGMIDLDTSKSTRSGKNGEKPQQENGIQKHRTALPAPMFT
  RSDFSVWSILKKCIGLELSKITMPIAFNEPLSFLQRITEYMEHVYLIHKASSQSQPLERMQSVAAFAVSA
  VASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFYSEGLNQDFRFHGSIYPKLKFWGKS
  VEAEPRGTITLELLKHNEAYTWTNPTCCVHNVILGQLWIEQYGIVEIVNHRTGDKCILHFKPCGLFGKEL
  HRVEGYIQDKNRKKLFIMYGKWTECLWGIDPASYESFKKQEKRGDQARKAKMDDGPEKANSDVPGDVADD
  VPVAQETVQVIPGSKLLWRINSRPPNSAQMYNFTSFTVSLNELESGMEKTLPPTDCRLRPDIRGMENGNM
  DLASQEKERLEEKQREARKERAKEDAEWRTRWFSPGNNPYTGAPDWLYAGHYFERNFSDCPDIY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144500
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144500.1
• RefSeq Size: 2746 bp
• RefSeq ORF: 1455 bp
• Locus ID: 228983
• UniProt ID: Q8BX94
• Cytogenetics: 2 H4
• Gene Summary: Intracellular transport protein that binds sterols and phospholipids and mediates lipid transport between intracellular compartments. Increases plasma membrane cholesterol levels and decreases phosphatidylinositol-4,5-bisphosphate levels in the cell membrane. Binds phosphoinositides, such as phosphatidylinositol-4,5-bisphosphate. Exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Binds cholesterol, dehydroergosterol, 22(R)-hydroxycholesterol and 25-hydroxycholesterol (in vitro).[UniProtKB/Swiss-Prot Function]