Dusp10 (NM_022019) Mouse Tagged ORF Clone

CAT#: MG207734

Dusp10 (tGFP-tagged) - Mouse dual specificity phosphatase 10 (Dusp10)

Product Images

Circular map for MG207734

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dusp10
• Synonyms: 2610306G15Rik; AI158871; MKP-5; MKP5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207734 representing NM_022019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCATCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCTGTCCGACCTCAGGATCTCA
  ACCTTTGTTTAGACTCAAGCTACCTTGGCTCTGCCAGCCCAGGTAGTGGTAGCCACGCTCCTGTCCTTGC
  CACCGCCGTTGTGACCCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCC
  CTGAATTGCGGATGCAGCAGCACTAGCTGCTGCACTGTGGCAACCTACGACAAGGATCATCAGGCCCAGA
  CTCAAGCCATTGCTGCAGGCACTGCCACCACCGCCATAGGAACCTCGACCACCTGCCCTGCTAACCAGAT
  GGTCAACAACAATGAAAACACAGGCTCTGTGTTAAGTCCATCGGGTGGGGTGGGCAGCCCTGTGTCAGGG
  ACTCCCAAGCAGCTAGCCAGCATCAAGATCATCTACCCCAATGACCTGGCAAAGAAGATGACCAAATGCA
  GCAAGAGTCACCTGCCCAGCCAGGGTCCCGTCATCATCGATTGCAGGCCTTTCATGGAGTACAACAAGAG
  TCACATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAGCCGGCGAAGGCTGCAGCAGGGCAAG
  ATCACTGTCTTAGACTTGATTTCCTGTAGGGAAGGCAAAGACTCTTTCAAGAGGATCTTTTCCAAAGAAA
  TTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGTGTGACACCATCCCAGCCACTTCACATAGTCCT
  TGAGTCCCTGAAGAGAGAAGGCAAAGAACCCCTGGTATTGAAAGGGGGACTCAGTAGTTTTAAACAGAAC
  CATGGAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGTGGTGCATCTGCGG
  CCTCGAGCATGCTACCTCAGTCTGTCCCCACCACCCCTGACATCGAGAACGCAGAGCTAACGCCCATCCT
  GCCCTTCCTGTTCCTCGGCAATGAGCAGGATGCTCAGGACCTAGACACCATGCAGAGGCTCAACATCGGC
  TATGTCATCAACGTCACCACGCACCTTCCTCTGTACCATTATGAGAAAGGCCTCTTCAACTACAAGAGGC
  TGCCAGCCACAGACAGCAACAAACAGAACCTGCGGCAGTACTTTGAAGAGGCCTTCGAGTTCATCGAGGA
  AGCTCACCAGTGTGGGAAGGGCCTTCTCATCCACTGCCAGGCCGGCGTGTCCCGATCCGCCACCATCGTC
  ATCGCCTACTTGATGAAGCACACACGGATGACCATGACTGACGCTTACAAATTCGTCAAAGGCAAACGAC
  CAATTATTTCCCCGAACCTCAACTTCATGGGGCAGTTGCTGGAATTTGAGGAAGACCTAAACAACGGCGT
  GACGCCAAGAATCCTTACACCAAAGCTCATGGGCATGGAGACAGTTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207734 representing NM_022019
  Red=Cloning site Green=Tags(s)
  
  MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSASPGSGSHAPVLATAVVTLKAANLTYMPSSSGSARS
  LNCGCSSTSCCTVATYDKDHQAQTQAIAAGTATTAIGTSTTCPANQMVNNNENTGSVLSPSGGVGSPVSG
  TPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGK
  ITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVTPSQPLHIVLESLKREGKEPLVLKGGLSSFKQN
  HGNLCDNSLQLQECREVGGGASAASSMLPQSVPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIG
  YVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIV
  IAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGMETVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022019
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022019.2, NP_071302.2
• RefSeq Size: 2713 bp
• RefSeq ORF: 1452 bp
• Locus ID: 63953
• UniProt ID: Q9ESS0
• Cytogenetics: 1 H5
• Gene Summary: Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.[UniProtKB/Swiss-Prot Function]