C730048C13Rik (BC050265) Mouse Tagged ORF Clone

CAT#: MG207692

C730048C13Rik (tGFP-tagged) - Mouse RIKEN cDNA C730048C13 gene (cDNA clone MGC:54808 IMAGE:5044850)

Product Images

Circular map for MG207692

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C730048C13Rik
• Synonyms: AA986766; C730048C13Rik; UST1R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207692 representing BC050265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTTCAGGAACTCCTGAATCAAGTTGGAAGCCTAGGAAGATTCCAGATCCTTCAGATCTCTTTTG
  TTCTCTTCCTCGTAGGCCTTGTGGTACCTCATATTACAATGGAGAACTTTACTGCAGCCATTCCCAATCA
  TCGCTGCTGGGCCCCCATCCTTGACAATGACACTGCCTCTGATAATGGCAGTAGGATCCTGAGCCAAGAT
  GACCTCCTGAGGATCTCCATCCCCCTGGATTCCAACCTGAGACTGGATAAATGTCGTCGTTTTGCCCAAC
  CACAGTGGCATCTTCTTCATTTGAATGGCACTTTCTCCAATGAGACAGAGCCAGACACTGAGCCCTGTGT
  GGATGGTTGGGTGTATGACAGGAGCAACTTCCTTTCTACCATTGTGACTGAGTGGGACCTGGTGTGTGAA
  TCTCAGGCACTGAATTCTGTTGCTAAATTTTCATTCATGATTGGCCTATTTATAGGGGCTATCATATGTG
  GCCATTTGTCAGACAGGTTTGGGAGGAAGTTCATTTTAACATGTGCTTTATTACAATTTGCCATCACTGA
  AACCTGTGTAGCTTTTGCTCCCTCCTTCTTCATCTACTGCTTACTTCGATTCCTGGCAGGAATGTCTGTG
  GAACCCATATCAGTAAATAGTCACTTGCTCATGTTAGAATGGACAAGCCCTAAATTCCTAGGCATGGTGG
  CAGTGCTTACATCTTGTGCTGCTAGTATCGGATACATGATATTGGCAGGCCTGGCTTTTCTATTTAGAAT
  CTGGCGTCACCTCCAGCTAGCAATGTCTGTGCCAATATTCTTCTTCCTTATACTCACAAGGTGGATGTCA
  GAGTCGGCTCGGTGGCTGATTGTGACCAACAAACCCCAGAAGGGCTTAAAGGAACTTAGAAAAGTTGCAC
  ACATGAATGGAATGAAAAATTCTGGAAATACCCTAACCATGGAGGTTGTGGAAGCAAGTATGAAGAATGA
  ACTTGAGGCAGCAAAAAGAAAATCTTCTCCAAGAGACTTGTTCCACACACCCATCTTACGAAAGCGGATA
  TGTGTCCTGTCCTTTATGAGATATTTATTTACAGTATCTATTTTTGGCCTATCACTTCACCTCCAACACC
  TGAGTACTAATATTATCCTGTTGCAGTTTCTCAGTAGTGCATTGGGCATACTAATTAGTGTTATTGGACA
  TTTTGTTCTTAATCACATGGGACGTAGAATAACTCAGCTGGTTCTCATGTCCCTGAGGGGAATATTCATG
  TTGACTGCTGTATTTGTACCTCAAGTTGGAAAGAGTCAAGACAAGCAAAGGAGGAGGATTCCATCATTAA
  AGTCACAAGGTTTTGAGGAACTCCTGGAATTGCCATCTGATCCAAGAGAAGAAAATTTTGTTCAGGACAT
  GGTGACAATTTTCTCAGATGATTACTCAGAATTCTTTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207692 representing BC050265
  Red=Cloning site Green=Tags(s)
  
  MAFQELLNQVGSLGRFQILQISFVLFLVGLVVPHITMENFTAAIPNHRCWAPILDNDTASDNGSRILSQD
  DLLRISIPLDSNLRLDKCRRFAQPQWHLLHLNGTFSNETEPDTEPCVDGWVYDRSNFLSTIVTEWDLVCE
  SQALNSVAKFSFMIGLFIGAIICGHLSDRFGRKFILTCALLQFAITETCVAFAPSFFIYCLLRFLAGMSV
  EPISVNSHLLMLEWTSPKFLGMVAVLTSCAASIGYMILAGLAFLFRIWRHLQLAMSVPIFFFLILTRWMS
  ESARWLIVTNKPQKGLKELRKVAHMNGMKNSGNTLTMEVVEASMKNELEAAKRKSSPRDLFHTPILRKRI
  CVLSFMRYLFTVSIFGLSLHLQHLSTNIILLQFLSSALGILISVIGHFVLNHMGRRITQLVLMSLRGIFM
  LTAVFVPQVGKSQDKQRRRIPSLKSQGFEELLELPSDPREENFVQDMVTIFSDDYSEFFF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050265
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050265.1
• RefSeq Size: 2029 bp
• RefSeq ORF: 1442 bp
• Locus ID: 319800
• Cytogenetics: 19 A